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Open culture method and application of pseudomonas stutzeri FSTB-5

A technology of FSTB-5 and Pseudomonas stutzeri, applied in the field of environmental microorganisms, can solve the problems of unclean processing, troublesome filtering methods, affecting the processing effect, etc., so as to reduce the culture cost, have strong salt tolerance, and achieve the processing effect. Good results

Active Publication Date: 2017-05-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the filtration method is cumbersome and the treatment is not clean; the acidification method kills miscellaneous bacteria while the growth of the dominant bacteria itself is also inhibited; the way of adding antibiotics also has a killing effect on the dominant bacteria, which in turn affects the treatment effect

Method used

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  • Open culture method and application of pseudomonas stutzeri FSTB-5

Examples

Experimental program
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Effect test

Embodiment 1

[0024] The open culture method of Pseudomonas stutzeri FSTB-5 of the present invention comprises five stages of bacterial strain activation, liquid seed solution cultivation, aeration cultivation, pure bacterial strain detection and dominant bacterial strain maintenance, and specific process is as follows:

[0025] (1) Strain activation: Pseudomonas stutzeri FSTB-5 was inoculated on a plate containing FSTB solid medium, cultured at 35°C for 48 hours, and then stored in a refrigerator at 4°C until use. The FSTB solid medium is: FeSO 4 •7H 2 O 25mg / L, NH 4 NO 3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.

[0026] (2) Liquid seed liquid culture: Prepare FSTB liquid medium, pack it into Erlenmeyer flasks, sterilize and cool to room temperature, use an inoculation loop to pick the activated strains in the slope and inoculate them into the Erlenmeyer flasks under a sterile environment. Incubate at 35°C for 48 ho...

Embodiment 2

[0031] The treatment process and operating conditions are the same as in Example 1, the difference is that in step (4) add rifamycin SV at a concentration of 5mg / L, and continue to add 5 culture cycles, and the floating microorganisms are drained and discharged Time to discharge, after which the normal open culture operation process is resumed. Collect the above cultured bacterial solution, spread it on a plate containing FSTB solid medium after dilution, count the grown colonies and count the percentage of colonies similar to FSTB-5.

Embodiment 3

[0033] The treatment process and operating conditions are the same as in Example 1, the difference is that in step (4), minocycline is added at a concentration of 25mg / L, and the addition is continued for 5 culture cycles. Ejected, after which the normal open culture operation process was resumed. Collect the above cultured bacterial solution, spread it on a plate containing FSTB solid medium after dilution, count the grown colonies and count the percentage of colonies similar to FSTB-5.

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Abstract

The invention relates to an open culture method of pseudomonas stutzeri FSTB-5. A bactericide is added in an open culture process of the pseudomonas stutzeri FSTB-5; the pseudomonas stutzeri FSTB-5 is preserved in China General Microbiological Culture Collection Center on June 1, 2015, with preservation number of CGMCC No.10940; and the bactericide includes one or more of lincomycin, minocycline, rifamycin SV, troleandomycin, vancomycin, aztreonam, nalidixic acid and the like. In accordance with the properties of the pseudomonas stutzeri FSTB-5, the open culture method provided by the invention, by adding the special bactericide in a process of conducting open culture of the pseudomonas stutzeri FSTB-5 with the addition of salt and COD containing wastewater, can significantly relieve microbe contamination, and the growth and propagation of the pseudomonas stutzeri FSTB-5 as well as a wastewater treatment effect cannot be affected.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to an open culture method and application of Pseudomonas stutzeri FSTB-5. Background technique [0002] Highly saline wastewater with a salt content higher than 1% from petroleum, chemical, pharmaceutical, and fertilizer industries generally contains high COD, and the continuous discharge of pollutants in wastewater into the environment will cause serious pollution problems. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water have been paid more attention by people. [0003] Physical or chemical treatment of COD in high-saline wastewater is costly and may cause secondary pollution. Traditional biological methods have great advantages in treating low-salinity wastewater, but in high-salt, high-acid-base, high-temperature or low-temperature adverse environments, the degradation ability of micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 郭志华高会杰孙丹凤赵胜楠李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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