Paracoccus sp. and culture application thereof

A paracoccus and liquid culture medium technology, applied in the field of environmental microorganisms, can solve the problems that Paracoccus does not have salt tolerance, is not suitable for high-salt wastewater treatment, and has high production costs, maintains growth characteristics and tolerance characteristics, and improves The effect of wastewater treatment and the effect of reducing cultivation cost

Active Publication Date: 2017-05-10
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

The single strain described in this method does not have salt tolerance, and multiple strains need to be cultivated for compounding, the preparation process is complicated, and the production cost is hig

Method used

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  • Paracoccus sp. and culture application thereof
  • Paracoccus sp. and culture application thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0028] Example 2 Screening and separation and purification

[0029] The method of gradually increasing the salt concentration in the culture solution was used to domesticate and cultivate the activated sludge taken from a petrochemical enterprise in Yueyang City, Hunan Province for treating the wastewater from the production of epichlorohydrin for 15 days.

[0030] The above-mentioned domesticated activated sludge is taken and preliminarily separated in the enriched medium by the method of dilution coating, thereby obtaining 15 colonies with good growth status and different characteristics. The used enrichment medium contains the main components: COD is 1000mg / L, calcium ion concentration is 1000mg / L, total salt content is 10000mg / L, and the agar content in the solid medium used is 2.0%.

[0031] Then, the obtained colonies are transferred by the method of plate streaking, and streaking is transferred to the screening medium for further separation and screening. The main components ...

Example Embodiment

[0033] Example 3 Investigation of Salt Tolerance of Paracoccus FSTB-2

[0034] The culture method of Paracoccus FSTB-2 of the present invention includes: strain activation, liquid seed liquid culture, aeration culture, and the specific process is as follows:

[0035] (1) Strain activation: Paracoccus ( Paracoccus sp. ) FSTB-2 was inoculated into a plate containing FSTB solid medium, cultured at 35°C for 48 hours, and then stored in a refrigerator at 4°C for later use. The FSTB solid medium is: FeSO 4 • 7H 2 O 25mg / L, NH 4 NO 3 286mg / L, KCl 929mg / L, CaCl 2 2769mg / L, NaCl 21008mg / L, beef extract 5g / L, peptone 10g / L, agar 20g / L, pH 7.8.

[0036] (2) Liquid seed solution culture: prepare FSTB liquid culture medium, divide it into Erlenmeyer flasks, sterilize and cool to room temperature, use an inoculating loop to pick the activated strains from the plate in a sterile environment and inoculate them into Erlenmeyer flasks. Cultivate for 48 hours at 35°C. The FSTB liquid medium is: Fe...

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Abstract

The invention relates to paracoccus sp. and a culture application thereof. The strain is paracoccus sp. FSTB-2, collected at 'China General Microbiological Culture Collection Center' on June 1, 2015, and the preservation number is CGMCC No. 10938. The strain can be applied to efficiently removing COD (chemical oxygen demand) in salty wastewater with salt content of 1.0wt%-8.0wt%, and can grow at the high temperature of 40 DEG C. The invention further provides COD removing salt-tolerant bacteria which comprise the paracoccus sp. FSTB-2. The COD in the high-salt wastewater can be rapidly degraded by the paracoccus sp., the paracoccus sp. is high in salt resistance and good in treatment effect, combining strains are omitted, and the production cost of the salt-tolerant bacteria is greatly reduced.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a salt-tolerant deCOD paracoccus and its cultivation application. The bacterial strain is especially suitable for efficient removal of COD in high-salt wastewater. Background technique [0002] Highly saline wastewater with a salt content higher than 1% from petroleum, chemical, pharmaceutical, and fertilizer industries generally contains high COD, and the continuous discharge of pollutants in wastewater into the environment will cause serious pollution problems. The shortage of fresh water resources is becoming more and more serious, and the treatment and recycling of waste water have been paid more attention by people. [0003] Physical or chemical treatment of COD in high-saline wastewater is costly and may cause secondary pollution. Traditional biological methods have great advantages in treating low-salinity wastewater, but in high-salt, high-acid-bas...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
Inventor 郭志华高会杰孙丹凤赵胜楠李宝忠
Owner CHINA PETROLEUM & CHEM CORP
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