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Salt-resistant pseudomonas stutzeri having low-temperature biological denitrification function

A technology of Pseudomonas stutzeri and microbial strains, applied in the field of microorganisms, can solve the problems of low denitrification activity, long processing time, lack of nitrite tolerance, etc., and achieve the effect of harmless energy and substances

Active Publication Date: 2014-05-07
南京曜动节能环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the aerobic denitrifying bacteria that have been reported so far have a weak ability to remove nitrite nitrogen. The strain has low denitrification activity in high-salinity water, the treatment time is too long, and the efficiency is not high.

Method used

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  • Salt-resistant pseudomonas stutzeri having low-temperature biological denitrification function
  • Salt-resistant pseudomonas stutzeri having low-temperature biological denitrification function
  • Salt-resistant pseudomonas stutzeri having low-temperature biological denitrification function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Acclimatization of Pseudomonas stutzeri DN-3 with low-temperature denitrification function

[0042] The source of the original strain: a Pseudomonas stutzeri DN-2 with normal temperature denitrification function, provided by the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, and the preservation number is CGMCC No.2917.

[0043] Low-temperature acclimatization: Inoculate 10 mL of the bacterial solution into a 500 mL Erlenmeyer flask containing 90 mL of acclimation medium at 10% inoculum volume, culture at 18°C ​​and shake at 120 r / min, and put a few glass beads in the Erlenmeyer flask to minimize weariness. The influence of the oxygen microenvironment on the test results, the culture period is 2 days, every 4 periods is a stage, and the nitrous acid in the medium is detected by N-(1-naphthyl)-ethylenediamine spectrophotometry after each stage Salt concentration until bacteria with a nitrite removal rate of 95% or above are o...

Embodiment 2

[0045] Nitrite removal by Pseudomonas stutzeri DN-3 at different temperatures

[0046] Pseudomonas stutzeri DN-3 was inoculated into the denitrification medium and cultured on a shaker for 24 hours, so that the amount of bacteria reached 10 9 CFU / mL or more (determine the concentration of Pseudomonas stutzeri by viable count method). After the cells were rinsed with centrifuged normal saline for three times, the supernatant was discarded, and an equal volume of normal saline was added to suspend the cells, and the bacterial suspension was inoculated with a 1% inoculation amount (volume ratio) to a nitrite nitrogen concentration of 330 mg / L. For the denitrification medium, set the culture temperature to 10, 15, 20, 25, and 30°C, pH 7.0, and 120r / min shaker culture; measure the nitrite nitrogen removal rate after 48 hours, and the results are shown in image 3 . Depend on image 3 It can be seen that the nitrite nitrogen removal rate of DN-3 can reach 100% in the temperature ...

Embodiment 3

[0048] Large scale cultivation of Pseudomonas stutzeri DN-3 in fermenter

[0049] The lawn of Pseudomonas stutzeri DN-3 dicyclics was picked up from the slant of denitrification agar and put into a 500ml Erlenmeyer flask with 100ml of denitrification medium, cultured on a shaker at 30°C and 120rpm for 12h. According to 5% inoculum size, inoculate 500ml of seed medium into a 10L fermenter with 9.5L of fermentation medium. According to the research results of the shake flask experiment, the culture conditions of the fermenter: 30°C, stirring speed 100r / min, ventilation rate 0.05vvm, inoculation amount 5%, fermentation medium composition: 0.5g / L NaNO 2 , 5.0g / LCH 3 COONa, 0.03g / L MgSO 4 , 0.01g / L MnSO 4 , 0.01g / L FeSO 4 , 0.75g / L K 2 HPO 4 , 0.25g / LNaH 2 PO 4 , the initial pH was adjusted to 8.5. After cultivating for 24 hours, the bacterial concentration was measured by live bacteria technique, and its concentration reached the maximum value of 1.2×10 11 CFU / mL, see ...

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Abstract

The invention relates to the field of microorganisms, and in particular relates to a bacterium having low-temperature denitration biological activity. The bacterium is characterized by being pseudomonas stutzeri DN-3, and is collected in the China General Microbiological Culture Collection Center with the collection number CGMCC No.8291 on October 8, 2013. The invention further relates to method for culturing the bacterium, preparation of a biological denitration agent by taking the bacterium as a major component and an application of the bacterium to nitrogen degradation in a water body.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacterium with low-temperature denitrification biological activity, a method for cultivating the bacterium, preparation of a biological denitrification agent mainly composed of the bacterium, and its application in nitrogen degradation in water bodies. Background technique [0002] In recent years, with the use of nitrogen fertilizer and the development of high-density aquaculture, it has brought huge economic benefits, but it has also caused serious environmental problems. Eutrophication caused by excessive nitrogen and phosphorus content frequently occurs in aquaculture, leading to deterioration of water quality and destruction of biological balance. The ammonia nitrogen and nitrite nitrogen in it are also highly toxic to farmed animals, especially It is nitrite nitrogen, and its conversion rate is too low under aquaculture conditions so that the accumulation of nitrite in the w...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/16
Inventor 周长林贾源宾张宁
Owner 南京曜动节能环保科技有限公司
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