Microbial agent, and its preparation method and application

A microbial inoculum, bioremediation technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as low degradation ability

Active Publication Date: 2011-11-23
宁夏中微泰克生物技术有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the existing microbial agents that have low degradation ability to petroleum hydrocarbons, to provide a microbial agent capable of efficiently degrading petroleum hydrocarbons and its preparation method and application

Method used

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  • Microbial agent, and its preparation method and application
  • Microbial agent, and its preparation method and application
  • Microbial agent, and its preparation method and application

Examples

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preparation example Construction

[0037] The present invention also provides a preparation method of the microbial agent, wherein the method comprises the preparation of Pseudomonas japonensis (Pseudoxanthomonas japonensis) with a preservation number of CGMCC No. Pseudomonas, Gordonia amicalis with preservation number CGMCC No.4794, Rhodococcus ruber with preservation number CGMCC No.4795 and alkanes with preservation number CGMCC No.4796 At least two species of Gordonia alkanivorans are inoculated in the culture medium for cultivation.

[0038] In a preferred embodiment, the preparation method includes: inoculating Pseudoxanthomonas japonensis (Pseudoxanthomonas japonensis) with a preservation number of CGMCC No. 4797 and at least one selected from the following strains in the culture medium : Pseudomonas (Pseudomonas) with preservation number CGMCC No.4793, Gordonia amicalis with preservation number CGMCC No.4794, Rhodococcus ruber with preservation number CGMCC No.4795 and Gordonia alkanivorans with a pres...

Embodiment 1

[0065] 1. Enrichment and isolation of petroleum hydrocarbon degrading bacterial strains

[0066] Weigh 5g of the oil-contaminated soil mixture and add it to a 250mL Erlenmeyer flask filled with 100mL enrichment medium, cultivate it at 28°C and 180r / min on a constant temperature shaker, and transfer 5mL of the culture medium to a fresh enrichment culture after 5 days base, cultured under the same conditions for 5 days, and continuously enriched and cultured for 25 days in this way, the culture solution was diluted serially and spread on solid PDA medium, and cultured at 28°C; after colonies grew on the plate, single colonies of different shapes were picked Separation and purification were carried out repeatedly by streak separation until a pure culture of a single strain was obtained, and the purified strain was preserved in the slant of beef extract peptone medium.

[0067] 2. Screening of strains with high degradation activity

[0068] Inoculate the above-mentioned purified ...

Embodiment 2

[0072] According to the experimental content and experimental methods recorded in "Common Bacteria Systematic Identification Manual" [4] and "Actinomycete Systematic Principles, Methods and Practice" [5], the preservation numbers obtained by screening are CGMCC No.4793, CGMCC The strains of No.4794, CGMCC No.4795, CGMCC No.4796 and CGMCC No.4797 were identified.

[0073] 1. Morphological analysis

[0074] The strains whose preservation numbers are CGMCC No.4793, CGMCC No.4794, CGMCC No.4795, CGMCC No.4796 and CGMCC No.4797 were analyzed for morphology and observed under an optical microscope. The results were as follows: Figure 1-16 shown.

[0075] The observation of the colony morphology was carried out on solid TSA medium. After the strains were streaked and inoculated, they were cultured at a constant temperature of 28°C for 24 hours to observe the colony morphology.

[0076] The colony of the strain with the preservation number CGMCC No.4793 is colorless and translucent...

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Abstract

The invention provides a microbial agent which comprises a medium and thalli, wherein, the thalli comprise at least two selected from the group consisting of pseudoxanthomonas japonensis with an accession number CGMCC No.4797, pseudomonas with an accession number CGMCC No.4793, gordonia amicalis with an accession number CGMCC No.4794, rhodococcus rubber with an accession number CGMCC No. 4795 and gordonia alkanivorans with an accession number CGMCC No.4796. The invention also provides a preparation method for the microbial agent and application of the microbial agent in degradation of petroleum hydrocarbon, bioremediation of soil polluted by petroleum and bioremediation of water bodies polluted by petroleum.

Description

technical field [0001] The present invention relates to a kind of microbial bacterial agent and preparation method and application thereof; Applications in pollution, applications in bioremediation of oil-contaminated soils, and applications in bioremediation of oil-contaminated water bodies. Background technique [0002] In today's world, petroleum has become one of the most important energy sources for human beings. A large amount of petroleum and its processed products enter the soil, causing petroleum pollution in the soil, which brings harm to human beings and even the entire biosphere, thus becoming a worldwide environmental problem. In the study of oil pollution, bioremediation, as a potential high-efficiency and low-cost cleaning technology, is gaining more and more attention. [0003] Bioremediation (Bioremediation) refers to the controlled or spontaneous process of repairing the polluted environment or eliminating pollutants in the environment by catalyzing the de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C12R1/01C12R1/38A62D101/20C02F101/32
Inventor 郭萍朱昌雄田云龙刘雪李峰叶婧
Owner 宁夏中微泰克生物技术有限责任公司
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