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Pseudomonas stutzeri YZN-001 for removing ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body and application

A technology of Pseudomonas stutzeri and nitrite nitrogen, which is applied in the direction of bacteria, water pollutants, water/sewage treatment, etc., can solve the problems of increasing water treatment costs, slow growth rate, and long reactor start-up time

Inactive Publication Date: 2010-05-12
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, traditional biological denitrification also has its own shortcomings that are difficult to overcome: on the one hand, nitrous bacteria are chemoautotrophic bacteria, and their growth rate is slow, which leads to a long start-up time of the reactor and increases the cost of water treatment. Sensitive to changes in composition, pH and temperature etc. (Meiberg and Harder; 1978)

Method used

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  • Pseudomonas stutzeri YZN-001 for removing ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body and application
  • Pseudomonas stutzeri YZN-001 for removing ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body and application
  • Pseudomonas stutzeri YZN-001 for removing ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1. Isolation, screening and identification of Pseudomonas YZN-001 strain

[0054] 1. Isolation and screening of strains

[0055] 1. Culture medium preparation: Composition and ratio of liquid culture medium for heterotrophic nitrifying bacteria for separation and screening: (NH 4 ) 2 SO 4 0.5g, sodium succinate 7.12g, Vickers salt solution 50mL, add water to dissolve and add water to 1L, adjust the pH value of the medium to 7.5-8.0 (heterotrophic nitrifying bacteria solid medium components and proportions are the same as heterotrophic nitrifying bacteria). Liquid medium, just add 2% by weight of agar) to the medium, and sterilize by moist heat at 121°C for 30min.

[0056] Among them, the components and proportions of Vickers salt solution: K 2 HPO 4 5.0g, MgSO 4 ·7H 2 O 2.5g, NaCl 2.5g, FeSO 4 ·7H 2 O 0.05g, MnSO 4 ·4H 2 O, 0.05g, after dissolving, add water to dilute to 1L, and sterilize by moist heat at 121°C for 30 minutes.

[0057] 2. Selecti...

Embodiment 2

[0062] Example 2: Degradation test of ammonia nitrogen by Pseudomonas stutzeri YZN-001

[0063] Degradation experiment medium formula: (NH 4 ) 2 SO 4 0.5g, sodium succinate 5.95g, Vickers salt solution 50mL, add water to dissolve and make up to 1L, pH 7.5~8.0. Vickers salt solution: K 2 HPO 4 5.0g, MgSO 4 ·7H 2 O 2.5g, NaCl 2.5g, FeSO 4 ·7H 2 O 0.05g, MnSO 4 ·4H 2 O, 0.05g, after dissolving, add water to make up to 1L. The culture solution was put into a 250 mL conical flask, 100 mL per bottle, and sterilized by moist heat at 121°C for 30 min. According to the inoculum of 1%, the bacterial suspension of YZN-001 strain activated after 24 hours was inserted, cultured at 30°C, 150r / min shaker for 36h, sampling every 6 hours, and centrifuged at 10000r / min for 5min to determine the supernatant in The absorbance at 420nm was compared with the standard curve to calculate the ammonia nitrogen content in the culture solution at different times, and the degradation rate of...

Embodiment 3

[0065] Example 3. Degradation test of nitrate nitrogen and nitrite nitrogen by Pseudomonas stutzeri strain YZN-001

[0066] 1. Degradation of nitrate nitrogen

[0067] The medium formula used for degradation is (in volume of 1L): KNO 3 2g, sodium succinate 18.7g, Vickers salt solution 50mL, add water to dissolve and make up to 1L, adjust pH to 7.5-8.0, and sterilize by moist heat at 121°C for 30min. Vickers salt solution: K 2 HPO 4 5.0g, MgSO 4 ·7H 2 O 2.5g, NaCl 2.5g, FeSO 4 ·7H 2 O 0.05g, MnSO 4 ·4H 2 O, 0.05g, after dissolving, add water to make up to 1L. The culture solution was put into 250 mL conical flasks, each 100 mL, and sterilized by moist heat at 121° C. for 30 minutes. The bacterial suspension of YZN-001 strain activated for 24 hours was inserted at 3% of the inoculum, incubated at 30°C, 150r / min shaker for 36h, samples were taken every 6 hours, centrifuged at 10000r / min for 5min, and the supernatant was measured at 220nm and the absorbance value at 2...

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Abstract

The invention belongs to the technical filed of agricultural microorganisms, particularly relating to separate and screen Pseudomonas stutzeri YZN-001 for degrading ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body and application. The screened Pseudomonas stutzeri YZN-001 of the invention is preserved in China Center for Type Culture Collection (CCTCC), with the preservation number being CCTCC NO:M 209107. The strain can effectively degrade ammoniacal nitrogen, nitrate nitrogen and nitrous nitrogen in water body within the range of 20-37 DEG C, has no toxicity to chub, has good degradation function on ammoniacal nitrogen in water body at normal temperature, the microorganism of the invention is inoculated for 72 hours, the degrading ratios to ammoniacal nitrogen in domestic sewage and aquaculture wastewater are respectively up to 83.10% and 61.38%.

Description

technical field [0001] The invention belongs to the technical field of agricultural application microorganisms, in particular to the screening and application of a strain of Pseudomonas stutzeri YZN-001 that removes ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in water bodies. technical background [0002] Nitrogen is an important pollutant in water pollution, including organic nitrogen and ammonia nitrogen, nitrite nitrogen and nitrate nitrogen from domestic sewage and agricultural waste. 3 , NH 4 - , N 2 , N 2 O, NO, NO 2 - , NO 2 , NO 3 - Equivalent states exist (Zheng and Ping, 2004). Studies have shown that, except for molecular nitrogen, the accumulation of all nitrogen cycle intermediates will have adverse effects on humans and the environment. A large amount of nitrogen compounds entering the water body will deteriorate the water quality, affect the development of fisheries and endanger human health. The harm is mainly manifested in: (1) ammonia n...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F1/58C02F101/16C12R1/38
Inventor 喻子牛张吉斌郝勃何进吴鹏霞刘子铎孙明
Owner HUAZHONG AGRI UNIV
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