Rhodopseudomonas palustris strains, application of rhodopseudomonas palustris strains, fungicide pesticide as well as preparation method and application of fungicide pesticide
A swamp Rhodopseudomonas strain technology, applied in the directions of botanical equipment and methods, biochemical equipment and methods, and applications, can solve the problems of difficult prevention and control of rice smut, outstanding chemical resistance, pesticide residues, etc. The effect of improving crop disease resistance, improving defense enzyme activity and low production cost
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Embodiment 1
[0040]A strain of Rhodopseudomonas palustris of the present invention, its name is Rhodopseudomonas palustris CS-1, specifically a strain of photosynthetic bacteria, and its preservation number in the China Center for Type Culture Collection is CCTCC No: M 2012224, the depository address is located at Wuhan University, China, and the deposit date is June 12, 2012. The initial sample of the bacterial strain of the present invention was collected on November 2, 2010 in a dry pond in Hunan Academy of Agricultural Sciences. When the initial sample was collected, in order to maintain the anaerobic environment and the freshness of the sample, the sediment at a depth of 3cm to 4cm in the pond sludge and the mixture of pond mud and water were intercepted. Take 5ml of the collected water sample, 5ml of the cement sample after precipitation, and 5ml of the sludge sample after mixing with an appropriate amount of sterile water. 7500Lx, temperature is 30 ℃ light incubator constant temper...
Embodiment 2
[0046] Example 2: biocontrol agent for Rhodopseudomonas palustris CS-1 and its preparation method.
[0047] A biocontrol agent for Rhodopseudomonas palustris strains, which is obtained after activation, seed cultivation and production cultivation of the Rhodopseudomonas paluscans strains obtained in the above-mentioned embodiment 1.
[0048] The specific preparation method of the biocontrol agent of the present embodiment comprises the following steps:
[0049] (1) Activation: the cryopreservation species of Rhodopseudomonas palustris CCTCC M 2012224 of embodiment 1 is cultivated by the double-layer plate culture method, and it is cultivated until red single colony occurs; the photosynthetic bacteria solid medium that adopts is: (NH 4 ) 2 SO 4 0.1%, MgSO 4 0.02%, NaHCO 3 0.5%, K 2 HPO 4 0.05%, NaCl 0.02%, yeast extract 0.15% and agar 1.5%, pH7.0~7.2;
[0050] (2) Seed culture: Put the red single colony cultivated in step (1) into a serum bottle with a volume of about 12...
Embodiment 3
[0054] Example 3: biocontrol agent for Rhodopseudomonas palustris CS-1 and its preparation method.
[0055] A biocontrol agent for Rhodopseudomonas palustris strain of the present invention, the biocontrol agent is the fermentation supernatant obtained after fermentation and centrifugation of the biocontrol agent obtained in Example 2.
[0056] The preparation method of the biocontrol agent of this embodiment specifically includes the following steps: take the biocontrol agent obtained in Example 2 and ferment and cultivate it for 6d to 8d to obtain the fermented liquid of Rhodopseudomonas palustris CCTCC M 2012224, and heat it at 8000rpm Centrifuge for 2 minutes under the same conditions, collect the supernatant after centrifugation, and filter the supernatant after centrifugation with a bacterial filter to obtain a sterile fermentation supernatant without photosynthetic bacteria cells, and store it at 4°C for future use.
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