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Pseudomonas and application thereof

A technology of pseudomonas and uses, applied in the field of microorganisms, can solve problems such as low phenol degradation rate, and achieve the effect of efficient degradation and solving low phenol degradation rate

Active Publication Date: 2015-02-18
SHENYANG RES INST OF CHEM IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to overcome the technical defect that the existing biological method can only degrade a single phenolic compound, and provides a new type of Pseudomonas, which can degrade a wide range of phenolic compounds, especially hydroquinone with high efficiency. At the same time, it can solve the problem of low phenol degradation rate when biologically treating the actual wastewater containing high phenol

Method used

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  • Pseudomonas and application thereof
  • Pseudomonas and application thereof
  • Pseudomonas and application thereof

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0024] (1) Preparation of bacterial total DNA: Genomic DNA of SY-HP-11 strain was extracted with Genome Extraction Kit of Tiangen Company, and used as a template for PCR reaction.

[0025] (2) PCR amplification of 16S rRNA gene: the amplification primers are as follows:

[0026] 27F: 5'-AgAgTTTgATCMTggCTCAg-3'[M=C,A]

[0027] 1492R: 5'-CggYTACCTTgTTACgACTT-3'[Y=T,C]

[0028] Middle primer: 533F: 5’—gTgCCAgCMgCCgCggTAA—3’

[0029] PCR reaction system:

[0030]

[0031] PCR reaction conditions:

[0032] a.94℃ for 3 minutes

[0033] b.94℃ for 1 minute

[0034] c.55℃ for 30 seconds

[0035] d.72℃ for 1 minute

[0036] e.72℃ for 1 minute

[0037] Wherein, after step a, three steps b, c, and d are performed for 30 cycles, and then step e is performed.

[0038] (3) Purification, cloning, sequencing and analysis of PCR products: PCR products were purified by agarose gel electrophoresis, connected to pMD18-T vector, transformed into E. The sequence was logged on the website ...

Embodiment 1

[0044] Under the conditions of pH 7.0 and temperature 30°C, inoculate the culture of SY-HP-11 strain in 100 mL of inorganic salt medium with hydroquinone as the sole carbon source, and the initial concentration of hydroquinone is 100 mg / L, 500mg / L, 1000mg / L, 1500mg / L, 2000mg / L, 2500mg / L, 3000mg / L, 150r / min shake culture and take samples after 72h, and use high performance liquid chromatography to determine the residual hydroquinone in the samples content to calculate the degradation rate. Taking the initial concentration of hydroquinone as the abscissa, the degradation rate of hydroquinone and OD 600 Value (thalline concentration) draws degradation and bacterial concentration curves for the ordinate, such as figure 1 Shown in , wherein, curve A is the degradation rate curve of hydroquinone, and curve B is OD 600 curve. from figure 1 It can be seen that when the hydroquinone concentration is lower than 2000mg / L, the SY-HP-11 bacterial strain has a good degradation effect o...

Embodiment 2

[0048] The measured growth of the SY-HP-11 strain after being cultured in different phenolic compound substrates is shown in Table 1, wherein, "+" means that there is colony growth, "++" means that the colony grows vigorously, " -" means no colony growth.

[0049] According to the results in Table 1, it can be seen that, in addition to being able to grow in hydroquinone medium, the SY-HP-11 strain can also grow in catechol, resorcinol, 4-methoxyphenol and 2, 6-dimethylphenol, indicating that the SY-HP-11 strain has a certain broad-spectrum for the removal of phenolic compounds.

[0050] Table 1 Growth of SY-HP-11 in different phenolic compounds

[0051] Catechol Resorcinol 4-methoxyphenol 2,6-Dimethylphenol + ++ ++ +

[0052] 3. Application of SY-HP-11 strain in the treatment of phenolic industrial wastewater

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses pseudomonas and application thereof. The pseudomonas is preserved in the China Center for Type Culture Collection on September 9, 2014, and is named pseudomonas sp.SY-HP-11 with the preservation number of CCTCC No.M2014400. The pseudomonas can be used for degrading different types of phenolic compounds, in particular hydroquinone, and can be used for treating phenol-containing industrial wastewater.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a pseudomonas and its application. Background technique [0002] Hydroquinone is a common water-soluble organic pollutant, which can be used in photographic developer, rubber and gasoline antioxidant, dye and pharmaceutical raw material. Phenol-containing industrial wastewater comes from wastewater discharged from petroleum, chemical, gas, coking, steel and phenol production plants. It has a large amount of discharge, has obvious toxicity, carcinogenic and mutagenic effects, and seriously pollutes water bodies. [0003] At present, there are mainly physical methods, chemical methods, and biological methods to treat industrial wastewater containing p-phenol at home and abroad. The physical method is suitable for treating wastewater with a low concentration of phenol, and the treatment effect on wastewater with a high concentration of phenol is poor; the chemical method has...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/34
CPCC02F3/34C02F2101/345C12N1/20C12N1/205C12R2001/38
Inventor 朱希坤彭湃李小明杨德玉戴速航李旭
Owner SHENYANG RES INST OF CHEM IND
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