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Short-cut nitrifying bacteria pseudomonad and application thereof

A Pseudomonas, short-range nitrification technology, applied in the direction of bacteria, chemical instruments and methods, biochemical equipment and methods, etc.

Inactive Publication Date: 2015-03-04
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At this stage, there is no report on short-range nitrification by a single strain, and mixed cultures are often used, such as activated sludge to achieve short-range nitrification, but it is easy to increase the uncontrollability of the entire reaction process

Method used

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  • Short-cut nitrifying bacteria pseudomonad and application thereof
  • Short-cut nitrifying bacteria pseudomonad and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Screening and identification of Pseudomonas sp. YH-01.

[0034] The experimental materials come from the silt on the banks of the Yi River in Linyi City, Shandong Province.

[0035] Take 0.1ml of the sludge dilution, spread it on the screening medium plate, culture it in a 30°C incubator, pick a single blue colony and repeat the streak purification 2-3 times to obtain the purified strain. After 16S rDNA identification, it belongs to Pseudomonas sp., see the phylogenetic tree figure 1 , having the sequence shown in 1 in the sequence table.

Embodiment 2

[0037] Experiment 1 of removing ammonia nitrogen by Pseudomonas sp. YH-01.

[0038] The processing object is the screening medium, and the operation mode is shaking culture in shake flasks.

[0039] The specific implementation steps are as follows: the strains are inoculated into LB medium, and cultured with shaking at 30°C until the bacterial concentration is O.D. 600 =2.0, inoculate in high ammonia nitrogen wastewater with 5% inoculum amount, culture at 25°C for 2 days, maintain dissolved oxygen above 3.0mg / L; centrifuge to take supernatant, according to "Nessler's reagent colorimetric method (GB7479-87)" Determination of ammonia nitrogen, according to "N-1-Naphthylethylenediamine Spectrophotometry (GB7493-87)" Determination of nitrite nitrogen, according to "Ultraviolet Spectrophotometry (DZ / T 0064.59-1993)" Determination of nitrate nitrogen, according to "Alkaline potassium persulfate digestion ultraviolet spectrophotometry (GB11894-89)" was used to determine total nitrog...

Embodiment 3

[0043] Experiment 2 of removing ammonia nitrogen by Pseudomonas sp. YH-01.

[0044] The treatment object is artificial high-nitrogen wastewater, and the operation mode is aeration cultivation in a 3L SBR (sequencing batch bioreactor) system.

[0045] The specific implementation steps are as follows: the strains are inoculated into LB medium, and cultured with shaking at 30°C until the bacterial concentration is O.D. 600 =2.0, inoculate in high ammonia nitrogen waste water with 1-5% inoculum size, cultivate 1 day at 20 ℃, maintain dissolved oxygen 2.5mg / L; The results are shown in Table 2 below.

[0046] Table 2

[0047]

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Abstract

The invention provides short-cut nitrifying bacteria pseudomonad and application thereof. The collection number of the short-cut nitrifying bacteria pseudomonad is CGMCC No.9412; the short-cut nitrifying bacteria pseudomonad can be used for removing ammonia nitrogen during sewage treatment particularly through the following steps of: inoculating a strain into an LB culture medium, and carrying out shaking culture at 30 DEG C till the bacterial concentration is O. D600=2.0; inoculating into high-ammonia nitrogen wastewater at an inoculation amount of 1%-5%, carrying out the shaking culture at 20-25 DEG C for 1-2 days, and keeping dissolved oxygen more than 2.5 mg / L; centrifuging to take a supernatant, measuring ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, total nitrogen and COD, wherein a result shows that the ammonia nitrogen removal effect is obvious, and certain total nitrogen and COD removal capacity is achieved. The short-cut nitrifying bacteria pseudomonad provided by the invention has very good application value in a sewage treatment project.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a short-range nitrifying bacterium Pseudomonas and its application. Background technique [0002] Ammonia nitrogen is one of the main pollutants in water bodies, and it is also one of the key monitoring indicators required by the state. With the development of modern industry and agriculture, the environmental pollution of ammonia nitrogen is becoming more and more serious. Free ammonia is toxic to living organisms. The conversion of ammonia nitrogen into nitrite nitrogen and nitrate nitrogen will pollute water sources and cause water eutrophication, which not only causes great damage to lakes and marine fishery resources, but also leads to pollution of groundwater sources and drinking water. According to reports, nitrate nitrogen in drinking water exceeding 10mg / L will cause infant methemoglobinemia, which will directly cause harm to human health. However, nitrite in water has a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38C02F101/38
CPCC02F3/34C02F2101/38C12N1/205C12R2001/38
Inventor 李强徐树建刘金民李修岭
Owner LINYI UNIVERSITY
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