Pseudomonas mediterranea strain and application thereof

A technology for pseudomonas and bacterial fertilizers, applied in bacteria, organic fertilizers, microorganisms, etc., can solve the problems of restricting practical popularization and application, difficult implementation of inoculation technology, long production cycle of bacterial agents, etc. Plant senescence agent - ethylene concentration, the effect of promoting plant nutrient absorption, and promoting root elongation

Active Publication Date: 2012-12-19
HARBIN NORMAL UNIVERSITY
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  • Summary
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Problems solved by technology

However, some fungi cannot be purely cultured, the production cycle of the fungal agent is long, the cost is large, the inoculation technology is difficult to implement, and it cannot be so

Method used

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  • Pseudomonas mediterranea strain and application thereof
  • Pseudomonas mediterranea strain and application thereof
  • Pseudomonas mediterranea strain and application thereof

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specific Embodiment approach 1

[0019] Specific embodiment 1: A strain of Pseudomonas mediterranea MJM-3 in this embodiment is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation address is No. 1, Beichen West Road, Chaoyang District, Beijing No. 3, the deposit date is June 26, 2012, and the deposit number is CGMCC NO.6292.

[0020] In this embodiment, Pseudomonas mediterranea (Pseudomonas mediterranea) MJM-3 is a Gram-negative bacterium, which forms round, opaque, light yellow, smooth protrusions, neat edges, and sticky colonies on the ADF medium (such as figure 1 shown).

[0021] Perform Gram staining, catalase, methyl red, acetylmethylmethanol, starch hydrolysis, indole, citrate on the isolated strain MJM-9 according to the Manual for the Identification of Common Bacterial Systems and the Berger's Handbook of Bacteria The detection and identification of physiological and biochemical experiments. The results showed that Pseud...

specific Embodiment approach 2

[0024] Embodiment 2: A strain of Pseudomonas mediterranei of this embodiment is used as bacterial fertilizer for crop cultivation.

[0025] The Pseudomonas mediterranea MJM-3 of this embodiment has ACC deaminase activity, produces plant growth hormone IAA, and synthesizes siderophiles; it can effectively promote plant nutrient absorption and regulate plant nutrition in a saline-alkali stress environment. Growth and improve the ability of plants to resist stress under adverse conditions. Its application in crop cultivation can improve fertilizer efficiency and increase yield.

[0026] The Mediterranean Pseudomonas (Pseudomonas mediterranea MJM-3) of this embodiment can inhibit or alleviate the adverse effects of plant diseases on plants by indirectly secreting siderophores, etc., and directly stimulate and regulate plant hormones by producing plant hormones and enzymatically reducing ethylene levels. Growth. The PGPR of the 1-aminocyclopropane-1-carboxylic acid (1-aminocyclopr...

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Abstract

The invention relates to a pseudomonas mediterranea strain and application thereof. The pseudomonas mediterranea MJM-3 is preserved in the general microorganism center of the China microorganism culture preservation management committee, the preservation address is No.3 of No.1 courtyard of Beichen West road of Chaoyang district of Beijing City, the preservation date is 26th July, 2012, and the preservation number is CGMCC NO.6292. The pseudomonas mediterranea strain is used as bacterial manure for growing crops. The pseudomonas mediterranea strain has ACC (1-aminocyclopropane-1-carboxylate) deaminase activity, and can secrete auximone IAA (indole acetic acid) and siderophores. Accordingly, the concentration of ethylene in a plant under the condition of environmental stress can be reduced, growth and development of a root system of the plant are promoted, iron absorption of the plant is also promoted, and growth condition of the plant is improved. Besides, when applied to agriculturalproduction, the pseudomonas mediterranea strain can promote growth of plants, yield of crops is increased, and stress resistance of the crops is strengthened.

Description

technical field [0001] The invention relates to a strain of Pseudomonas mediterranei and its application. Background technique [0002] There are large areas of saline-alkali land and secondary salinized soil in the world. With the intensification of the contradiction between population, food, land and energy, especially with the construction of some large-scale water conservancy projects, the problem of secondary salinization is becoming more and more serious. Therefore, the development of salinized land and the prevention and control of secondary salinization very urgent. [0003] Soil salinization is one of the ecological crises faced by human beings. Salt stress will not only affect plant growth and yield, but high concentration salt stress will cause plant biomass reduction or even death. Salt-alkali stress will affect almost every important physiological process of plants. Under salt stress, plant growth is inhibited, metabolism is affected, and the photosynthetic c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/38
Inventor 郭长虹马嘉敏刘佳莉蔡洪生管鹏
Owner HARBIN NORMAL UNIVERSITY
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