Pseudomonas sp. strain and use thereof

A technology of Pseudomonas strains, applied in Pseudomonas strains and its application fields, which can solve the problems of long crop rotation, difficult implementation, and outbreak of pathogenic bacteria

Inactive Publication Date: 2010-12-29
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The paddy and dry rotation requires a long rotation period and no other hosts nearby to achieve good results, so it is difficult to implement
After the soil is sterilized, a soil biological vacuum is formed. Once the soil is infected by pathogenic bacteria, it will lead to a large outbreak of pathogenic bacteria and cause more serious consequences.

Method used

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  • Pseudomonas sp. strain and use thereof
  • Pseudomonas sp. strain and use thereof
  • Pseudomonas sp. strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the separation and purification of bacteria

[0031] Bacteria were isolated and purified according to the following test steps:

[0032] 1 medium

[0033] Beef extract peptone agar medium: beef extract 3g, peptone 10g, NaCl 5g, agar 15-20g, H 2 O 1000ml, pH 7.0-7.2. Sterilize at 121.3°C for 20 minutes.

[0034] 2 test steps

[0035] 2.1 Preparation of medium

[0036] Prepare the beef extract peptone agar medium as described in 1 to prepare the bacterial slant medium.

[0037] 2.2 Preparation of soil dilution

[0038] (1). Weigh 10g of soil, add 100ml of sterile water with glass beads into a 500ml Erlenmeyer flask, shake for 15 minutes, that is, 10-1;

[0039] (2). Draw 10ml of soil suspension, add 90ml sterile water to 500ml triangular flask, that is, 10 -2 ;

[0040] (3). Draw 10ml from (2), add 90ml sterile water to 500ml triangular flask, that is, 10 -3 ;

[0041] (4). Draw 5ml from (3), add 45ml sterile water to 250ml triangular flask, that i...

Embodiment 2

[0051] Embodiment 2: Plate antagonism test of isolated bacteria to R. solanacearum of tomato bacterial wilt

[0052] 1. Medium

[0053] 1.1 Solid medium

[0054] Culture medium preparation for R. solanacearum: MgSO 4 7H 2 O0.3g, K 2 HPO 4 2.0g, Yeast extract (yeast extract) 4.0g, Casein hydrolysate (Luo protein hydrolyzate) 8.0g, Sucrose (sucrose) 10.0g, Agar (agar) 18g, Distilled water (distilled water) 1000ml.

[0055] Preparation of beef extract peptone agar medium: same as 1 in Example 1.

[0056] 1.2 Liquid medium

[0057] Bacterial liquid shake flask medium: beef extract peptone medium without adding agar.

[0058] Liquid shake flask culture medium for R. solanacearum of tomato: the culture medium for R. solanacearum does not need to add agar.

[0059] 2. Test steps

[0060] 2.1 Culture medium preparation

[0061] Prepare Ralstonia solanacearum medium (solid, liquid) to prepare the slant of the pathogen Larsia solanacearum; prepare beef extract peptone agar mediu...

Embodiment 3

[0074] Example 3: Biocontrol effect test of strain ②-29 fermented liquid on tomato bacterial wilt in potted plants In order to test the biocontrol effect of strain ②-29, this test was carried out.

[0075] Experimental steps:

[0076] 1. Seedling cultivation

[0077] 1.1 Preparation of seedling substrate

[0078] The substrate for raising seedlings is: peat: vermiculite: perlite = 7:1:0.5, then use tap water to adjust the water content to 60% to 70%, and set aside.

[0079] 1.2 Sowing

[0080] Put about three-quarters of the 1.1 prepared matrix in each hole of the seedling tray (90×60 size), press lightly with your hands, then sprinkle 50 tomato seeds evenly in each hole, and then spread a thin layer of Substrate, and cover the tray with a plastic bag to prevent water from evaporating, set aside.

[0081] 1.3 Emergence

[0082] Put the sowing trays prepared in 1.2 into an artificial climate box for cultivation at a temperature of 25°C, put in a large basin of water to moi...

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Abstract

The invention provides a pseudomonas sp. strain 2-29, which belongs to the class of pseudomonas sp. and was collected with a collection number of CGMCC No.3331 in China General Microbiological Culture Collection Center (abbreviated as CGMCC) of Institute of Microbiology Chinese Academy of Sciences located on Datun road in Chaoyang district of Beijing, on October 2009. The invention also provides the use of the pseudomonas sp. strain 2-29 in the prevention and control of tomato ralstonia solanacearum. For solving the problem that an effective chemical for treating tomato ralstonia solanacearum lacks at present and the problem that pseudomonas sp. is susceptible to generating medicament resistance to chemical pesticide, a strain having an antagonistic effect on tomato ralstonia solanacearum is screen through screening tomato ralstonia solanacearum antagonists and the test of the control effect of the tomato ralstonia solanacearum antagonists; on this basis, field tests can be further performed for developing a mature microbial fertilizer; and thus, the pseudomonas sp. strain 2-29 has a great significance for reducing the use of a large amount of fertilizer and pesticide and reducing agricultural pollution sources.

Description

technical field [0001] The invention relates to the field of biology, in particular to a pseudomonas strain and application thereof. Background technique [0002] Tomato bacterial wilt is a devastating soil-borne disease caused by Ralatonia solanacearum (smith) Yabuuchi et al] worldwide, in tropical and subtropical countries and regions, such as South America, Japan, India , the Philippines, my country's Taiwan, Guangdong, Zhejiang, Fujian, Jiangsu, Hubei, Sichuan, Chongqing and other regions have seriously occurred. The disease can cause large areas of wilting and death of plants. The incidence rate of general fields is 10% to 15%, and the incidence rate of severe disease fields is as high as 80% to 98.5%, resulting in a serious decline in tomato yield and major economic losses. [0003] R. solanacearum is a soil-inhabitant fungus that can survive in soil for a long time without a host. R. solanacearum mainly invades plants from the root system, and can also invade from st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/38
Inventor 朱凤香冯明光薛智勇叶青静王卫平陈晓旸洪春来吴传珍
Owner ZHEJIANG UNIV
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