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Prepn process of strain and prepn for controlling blight of vegetable biologically

A biocontrol strain and strain technology, applied to the microbial strain Pseudomonas chloropinus GP72, the application of plant soil-borne fungal diseases, the field of preparation of biocontrol strains and preparations, can solve the problem of biological pesticides that do not control vegetable diseases and other issues to achieve the effect of avoiding soil pollution

Inactive Publication Date: 2007-03-21
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no report on the microbial preparations that can effectively prevent and control vegetable diseases in the world, and there are no reports on biopesticides that can prevent and control vegetable diseases.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Acquisition of bacterial strains: collect several different sweet pepper rhizosphere soil samples, weigh 1g sweet pepper root hairs and 1g soil samples attached to the root hairs and dissolve them in 50ml 1 / 3 KMB liquid medium, and add antibiotic: ampicillin (40μg / ml), chloramphenicol (13μg / ml), cycloheximide (100μg / ml), shake at 230rpm / min at 28°C for 1 hour, mix the 6 parallel samples, and take 200μl of the culture solution to apply Spread on KMB agar plates containing the same dose of antibiotics. After culturing at 28°C for 18 hours, after the growth of a single colony, the antibacterial test of Phytophthora capsici was carried out by asynchronous culture method, and the strong antagonistic strain with the maximum antibacterial activity was screened out, which was identified as Pseudomonas chlororaphis GP72 (Pseudomonas chlororaphis) .

[0030] The strain is now preserved in the General Microorganism Center (CGMCC) of China Committee for Culture Collection of Mi...

Embodiment 2

[0037] 1. Preparation of the bacterial strain: pick up the sample of the frozen preserved bacterial strain (Pseudomonas chloropinus GP72) with an inoculation needle, add 0.2ml of sterile water and wait for the sample to dissolve, then transfer to KMB medium for 16 hours of cultivation.

[0038] 2. Preparation of microbial preparations: transfer the above-mentioned cultures into shake flasks for scale-up cultivation, inoculate with 3% inoculum, shake and culture in a water-bath shaker as seed liquid, and then carry out shake-flask scale-up production or fermentation production preparation For the microbial preparation, the inoculation amount is 4%, the culture temperature is 28° C., the growth pH value is 7.5, and the fermentation period is 60 hours.

[0039] The components of the above KMB medium are: 20g of peptone, 15g of glycerol, KH 2 PO 4 0.50g, anhydrous MgSO 4 0.75g agar 16g, pH7.5, dilute to 1L.

[0040] The application of the obtained microbial preparation to the...

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Abstract

The present invention relates to preparation process of strain and preparation for controlling blight of vegetable biologically. One kind of Pseudomonas chlororaphis GP72 is cultured and screened with sweet pepper rhizoid and its attached soil sample and KMB culture medium with antibiotic added, and preserved in the number of CGMCC No. 1748. The freeze preserved strain is activated and amplifying cultured to obtain microbial preparation, which may be used for preventing and controlling blight of chili, cucumber, wax gourd, etc; for preventing and controlling rape sclerotinia rot, cucumber fusarium wilt, water melon fusarium wilt, etc; and as vegetable growth promoter to raise the yield of vegetable.

Description

technical field [0001] The present invention relates to a preparation method of biocontrol strains and preparations for preventing and treating vegetable blight, in particular to a microbial strain Pseudomonas chlororaphis GP72 (Pseudomonas chlororaphis), a method for preparing microbial preparations using the strain, and the use of the microbial preparations in Application in the prevention and control of vegetable blight and other plant soil-borne fungal diseases. It belongs to the field of biological control and biological engineering technology. Background technique [0002] Our country has a large vegetable planting area and high yield. In 2005, the national vegetable planting area was 17.74 million hectares, with a total output of 563 million tons. It is difficult to estimate the economic loss of vegetables caused by pests and diseases every year, among which vegetable blight is a common vegetable disease. [0003] Vegetable blight is caused by the fungus infection ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02C12R1/38
Inventor 张雪洪刘海明蒋海霞许煜泉
Owner SHANGHAI JIAO TONG UNIV
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