Pseudomonas chlororaphis and culture method and applications thereof

A technology of Pseudomonas aeruginosa and an application method, applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of reducing soil fertility, reducing the uniformity of seedling emergence, producing phytotoxicity, etc. The effect of root vitality, promoting plant growth and reducing side effects

Active Publication Date: 2019-08-20
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, seed coating agents also have certain side effects, such as: affecting seed emergence, reducing the uniformity of emergence, and even causing deformed seedlings; producing p

Method used

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  • Pseudomonas chlororaphis and culture method and applications thereof
  • Pseudomonas chlororaphis and culture method and applications thereof
  • Pseudomonas chlororaphis and culture method and applications thereof

Examples

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Embodiment 1

[0041] This embodiment provides a kind of Pseudomonas chloropinus screened from the plant rhizosphere growth-promoting bacterial flora in wheat roots and rhizosphere soil thereof, and the method for obtaining and cultivating Pseudomonas chloropinus comprises the following steps:

[0042] Acquisition of bacterial strains: Cut 1g of the root of wheat belt soil with excellent growth and health, grind it with a mortar, and dilute it to 10,000 times with sterile water. Spread the diluted mixture evenly on a 1 / 10TSA medium plate. Place it upside down in a constant temperature incubator at 28°C for 48 hours. After the strains grow out, use a sterile toothpick to pick bacterial colonies with different sizes, colors and shapes, and streak them on the TSA medium plate to separate and purify them. It is stored in a -70°C ultra-low temperature refrigerator; the isolated and purified strains are repeatedly screened through laboratory tests and field tests to obtain strains that can prevent ...

Embodiment 2

[0046] The present embodiment provides a kind of Pseudomonas chlororaphis bacterium liquid, and it is that the Pseudomonas chlororaphis Pc-HG28-5 that embodiment 1 provides and concentration are 0.1mol / l sterile magnesium sulfate solution preparations suspension, and the concentration of Pseudomonas aeruginosa in the Pseudomonas aeruginosa liquid is 2 × 10 8 CFU / ml.

Embodiment 3

[0048] This embodiment provides a kind of Pseudomonas chloroacinatus bacterial liquid, which is obtained by mixing a buffer solution containing magnesium sulfate with the Pseudomonas chloroacinatus, and the Pseudomonas chloroacinatus bacterial liquid contains The concentration of Pseudomonas was 1 x 10 8 CFU / ml; based on 100 parts by mass, the buffer solution includes: 0.02 part of sodium citrate, 0.15 part of ammonium sulfate, 0.5 part of dipotassium hydrogen phosphate, 0.1 part of potassium dihydrogen phosphate, 0.03 part of magnesium sulfate, 0.5 part of glycerin, The balance is sterile water.

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Abstract

The invention provides pseudomonas chlororaphis. The pseudomonas chlororaphis is pseudomonas chlororaphis Pc-HG28-5, and is preserved in the China General Microbiological Culture Collection Center, wherein the preservation number is CGMCC No.14208. The pseudomonas chlororaphis is a plant growth promoting rhizobacterium which is separated and screened out from wheat roots, and can be cultured in apeptone culture medium. The pseudomonas chlororaphis can reduce a side effect of a seed coating agent, prevent epidemic disease, and reduce the number of harmful fungi in plant rhizosphere soil.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a pseudomonas chloropin and its cultivation method and application. Background technique [0002] Plant Growth Promoting Rhizobacteria (PGPR for short) refers to the presence in the plant rhizosphere environment, through the direct effects of producing plant hormones, dissolving inorganic phosphorus, and indirect effects such as competing for ecological niches and inducing plant system resistance. A class of beneficial bacteria that grow on plants. In recent years, a large number of studies have shown that PGPR strains can effectively colonize plant roots and rhizosphere soils, have significant effects on growth promotion, disease prevention and production increase, and at the same time regulate the micro-ecological environment of plant rhizosphere soils and alleviate ecological pollution. It is one of the effective ways for the sustainable development of ecological agricultu...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01C1/06C12R1/38
CPCC12N1/20A01C1/06C12R2001/38C12N1/205
Inventor 申顺善朴凤植王娟文才艺闫凤鸣
Owner HENAN AGRICULTURAL UNIVERSITY
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