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Microbial agent for preventing and treating apple ring rot and preparation method thereof

A microbial agent, apple ring disease technology, applied in the field of microbial agent for the prevention and treatment of apple ring disease and its preparation, can solve the problems of high labor intensity for fruit farmers, harsh pesticide application environment, microbial death, etc., to ensure microbial activity , enhance the ability to resist adverse environments, and promote the effect of apple growth

Pending Publication Date: 2019-10-11
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN102805107B discloses a microbial preparation for preventing and treating apple ring spot, which is obtained by activation and fermentation of Bacillus subtilis, and its control effect still has a lot of room for improvement; moreover, multiple applications are required, including spraying during the flowering period in April. Apply or smear the rough bark of the trunk, spray the fruit surface before fruit bagging in June, and spray or smear the trunk diseased tissue in July and August when there is a lot of rain. The process is cumbersome and the labor intensity of the fruit grower is high.
One of the important reasons for this is that the application of microbial agents is more sensitive to the environment, and the poor application environment is likely to cause a large number of microorganisms to die, which in turn will lead to the deterioration of the control effect or even to zero.

Method used

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  • Microbial agent for preventing and treating apple ring rot and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0032] A microbial agent for preventing and treating apple ring spot, comprising two parts, a composite microbial powder and a gel carrier loaded therewith, in parts by weight, the composite microbial powder comprising: 1 part of Bacillus cereus ACCC03288 bacterial powder, 0.2 part of Trichoderma harzianum ACCC30371 bacterial powder, 0.3 part of Pseudomonas chloropinus ACCC19853 bacterial powder, 0.1 part of Paecilomyces lilacinus ACCC30639 bacterial powder, 0.3 part of Streptomyces hygroscopicus ACCC40018 bacterial powder; the gel carrier is obtained by It is prepared by the following method: firstly add acrylamide and sodium acrylate to polyvinyl alcohol aqueous solution, and obtain a gel intermediate through ultraviolet irradiation reaction, then soak the gel intermediate in sodium alginate solution, and drop calcium chloride solution , cross-linking reaction, blocking and placing, and washing to obtain a gel carrier.

[0033] The mass ratio of composite microbial powder to...

Embodiment 2

[0044] A microbial agent for preventing and treating apple ring spot, comprising two parts, a composite microbial powder and a gel carrier loaded therewith, in parts by weight, the composite microbial powder comprising: 1 part of Bacillus cereus ACCC03288 bacterial powder, 0.3 part of Trichoderma harzianum ACCC30371 bacterial powder, 0.5 part of Pseudomonas chloropinus ACCC19853 bacterial powder, 0.2 part of Paecilomyces lilacinus ACCC30639 bacterial powder, 0.5 part of Streptomyces hygroscopicus ACCC40018 bacterial powder; the gel carrier is obtained by It is prepared by the following method: firstly add acrylamide and sodium acrylate to polyvinyl alcohol aqueous solution, and obtain a gel intermediate through ultraviolet irradiation reaction, then soak the gel intermediate in sodium alginate solution, and drop calcium chloride solution , cross-linking reaction, blocking and placing, and washing to obtain a gel carrier.

[0045] The mass ratio of composite microbial powder to...

Embodiment 3

[0056] A microbial agent for preventing and treating apple ring spot, comprising two parts, a composite microbial powder and a gel carrier loaded therewith, in parts by weight, the composite microbial powder comprising: 1 part of Bacillus cereus ACCC03288 bacterial powder, 0.2 part of Trichoderma harzianum ACCC30371 bacterial powder, 0.5 part of Pseudomonas chloropinus ACCC19853 bacterial powder, 0.1 part of Paecilomyces lilacinus ACCC30639 bacterial powder, 0.3 part of Streptomyces hygroscopicus ACCC40018 bacterial powder; It is prepared by the following method: firstly add acrylamide and sodium acrylate to polyvinyl alcohol aqueous solution, and obtain a gel intermediate through ultraviolet irradiation reaction, then soak the gel intermediate in sodium alginate solution, and drop calcium chloride solution , cross-linking reaction, blocking and placing, and washing to obtain a gel carrier.

[0057] The mass ratio of composite microbial powder to gel carrier is 1:30.

[0058]...

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Abstract

The invention provides a microbial agent for preventing and treating apple ring rot and a preparation method thereof. The microbial agent is obtained by using a gel carrier loaded with compound microbial powder. The compound microbial agent comprises bacillus cereus ACCC03288 powder, trichoderma harzianum ACCC30371 powder, pseudomonas chlororaphis ACCC19853 powder, paecilomyces lilacinus ACCC30639powder and thorn spore streptomyces hygroscopicus ACCC40018 powder. The growth and reproduction of apple ring rot germs are synergistically inhibited, nutrition support is provided for apple trees, and the growth of apples is promoted. The gel carrier is obtained by utilizing a cross-linking reaction of acrylamide and sodium acrylate, cross-linking sodium alginate and calcium chloride and introducing polyvinyl alcohol, can be naturally degraded, has a slow release effect on the compound microbial powder, and provides nutrition support for the microbial growth, so that the compound microbial powder has a better control effect. The microbial agent only needs to be applied once, thereby reducing the labor intensity.

Description

technical field [0001] The invention relates to the technical field, in particular to a microbial agent for preventing and treating apple ringworm and a preparation method thereof. Background technique [0002] Ring spot is one of the common diseases of apples, which is caused by the infection of weak parasites, including branch ring spot and fruit ring spot. The ringworm fungus survives the winter in the damaged branches with mycelia, pycnidia and asthecia. The hyphae can survive for 4 to 5 years in the stem diseased tissue, and produce spores between April and June every year, becoming the source of primary infection. From July to August, the spores are distributed more; the ability to produce spores in the diseased part is strong in the first three years, and then gradually weakens. The conidia are mainly spread with the splash of rainwater, and the range is generally not more than 10 meters. The fungus can infect young fruit about 10 days after flower fall. The amoun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C12N11/10C12N11/04C12N1/20C12N1/14A01N63/04A01N63/02A01P3/00C12R1/085C12R1/885C12R1/38C12R1/79C12R1/465
CPCC12N11/08C12N11/10C12N11/04C12N1/20C12N1/14A01N63/10A01N63/30
Inventor 李如美高宗军李瑞娟戴争
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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