Method for preparing drug or gene carried stent

A gene and drug-loading technology, which is applied in the field of medical materials, can solve the problem that the collagen coating is easily eluted, and achieve the effects of reducing toxicity and immunogenicity, improving transfection efficiency, and increasing drug loading

Inactive Publication Date: 2007-05-16
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main problem at present is that the collagen coating is

Method used

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  • Method for preparing drug or gene carried stent

Examples

Experimental program
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Effect test

Example Embodiment

[0034] Example 1 A method for preparing a gene-carrying scaffold consists of the following steps:

[0035] (1) Pretreatment of the stent surface

[0036] ①The stent is immersed in acetone solution for ultrasonic cleaning for 30 minutes, and then replaced with isopropanol solution for ultrasonic cleaning for 30 minutes. After cleaning with double distilled water, it is soaked in 0.2N hydrochloric acid solution for 30 minutes, and rinsed with double distilled water for 3 times. Dry in an oven for later use, and sputter 20nm chromium and 50nm gold metal film on the dried support;

[0037] ②Fixed polymer: soak the bare gold stent in 0.1M 11-mercaptoundecyl alcohol (first dissolve 11-mercaptoundecyl alcohol in ethanol, then add water, and dissolve in 80:20 ethanol and water) for 30 minutes , To form a single-layer self-assembled film on the gold film, rinse with distilled water; then add 0.6M epichlorohydrin (dissolved in a 1:1 diglyme and 0.4M NaOH mixed solution) to react For 30 minu...

Example Embodiment

[0046] Example 2 A method for preparing a gene-carrying scaffold consists of the following steps:

[0047] (1) Same as Example 1;

[0048] (2) The solidification of gene molecules on the surface of the scaffold

[0049] a. Surface activation of the stent: Put the fixed polymer stent into a mixed aqueous solution of 0.01M N-hydroxysuccinimide and 0.01M ethyl-dimethylaminopropyl-carbodiimide and soak for 1 minute. Activated stent surface;

[0050] b. Immobilization: Put the surface-activated stent into a 1000g / L chitosan (carrier) aqueous solution with a pH of 3.6 and soak for 60 minutes, take it out, and put it in a 0.01M ethanolamine hydrochloride aqueous solution with a pH of 9 for 30 minutes. Block activation group;

[0051] c. Put 0.001g / L gene solution to be loaded and 0.001g / L Lipofectamine TM Soaked in 2000 mixed solution for 30 minutes, the gene solution is made by dissolving the gene in a PBS solution of pH 8. The Lipofectamine TM 2000 solution is Lipofectamine TM 2000 was...

Example Embodiment

[0054] Example 3 A method for preparing a gene-carrying scaffold consists of the following steps:

[0055] (1) Same as Example 1;

[0056] (2) The solidification of gene molecules on the surface of the scaffold

[0057] a. Surface activation of the stent: Place the fixed polymer stent into a mixed aqueous solution of 10M N-hydroxysuccinimide and 10M ethyl-dimethylaminopropyl-carbodiimide and soak for 60 minutes to activate the stent surface;

[0058] b. Immobilization: Put the surface-activated stent into a 0.1g / L chitosan (carrier) aqueous solution with a pH of 9 and soak for 5 minutes, take it out, and put it in a 10M ethanolamine hydrochloride aqueous solution with a pH of 8 for 1 minute. Block activation group;

[0059] c. Put in 1g / L gene solution to be loaded and 1g / L Lipofectamine TM Soaked in a mixed solution of 2000 for 120 minutes, the gene solution is made by dissolving the gene in a PBS solution with a pH of 5, and the Lipofectamine TM 2000 solution is Lipofectamine TM...

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Abstract

The invention relates to a method for preparing drug carrier or gene carrier support, wherein said method comprises (1), support surface pretreatment that splashing or plating gold, fixing macromolecule; (2), solidifying the drug or gene molecule on the surface of support that activating the support surface, loading carrier, sealing and solidifying the drug or gene. The invention uses laminated installment, connects mesh macromolecule skeleton on the support, to improve the drug carrier, reduce the toxicity of support and connecting molecule and the immunogen. The invention uses mesh molecule to connect carbohydrate, peptide, and antibody, to connect the drug and antibody firmly and release drug slowly. The invention uses mesh molecule static adsorption gene molecule and transfer medium molecule to improve the transfer efficiency of gene molecule.

Description

technical field [0001] The invention belongs to the field of medical materials, in particular to a blood vessel stent. Background technique [0002] Coronary artery embolism is one of the major diseases that endanger human health. Interventional therapy (PTCA) and bypass surgery are the main clinical treatment methods. Coronary artery stents in interventional therapy play an important role in the treatment of coronary artery embolism. Placement of stainless steel stents after interventional therapy can prevent the collapse of the expanded vessel wall and maintain smooth blood flow, but foreign body stimulation of the stents often leads to excessive growth of local endothelial cells, intimal hyperplasia, and finally re-embolization, which is clinically called restenosis ( RS), the incidence rate of postoperative vascular restenosis is as high as 30-50%, which seriously limits the long-term effect and wide application of treatment, and becomes a difficult problem to be solved ...

Claims

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Application Information

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IPC IPC(8): A61L31/16A61L31/10A61F2/82
Inventor 宋存先鲍军波欧惠超
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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