Ganoderma lucidum edgeworthia chrysantha promoter for promoting efficient edgeworthia chrysantha of agilawood plants

Abstract

The invention discloses a ganoderma lucidum edgeworthia chrysantha promoting agent for promoting efficient edgeworthia chrysantha of aquilaria plants, and belongs to the technical field of edgeworthia chrysantha of aquilaria plants. The component A is prepared from the following components in parts by weight: 20 to 40 parts of aspergillus niger microbial fermentation liquor, 15 to 30 parts of verticillium fulvum microbial fermentation liquor, 10 to 15 parts of fusarium oxysporum microbial fermentation liquor, 5 to 10 parts of ganoderma lucidum extract, 6 to 8 parts of seaweed extract, 2 to 8 parts of malic acid, 3 to 6 parts of fructose, 1 to 3 parts of pectinase and 0.5 to 2 parts of cellulase. Through the synergistic effect of the component A and the component B, the agilawood formation rate and quality of agilawood are effectively improved, the synergistic effect of microorganisms stimulates the agilawood formation potential of plants, accurate regulation and control of the plant hormone xylan emulsion provide conditions for synthesis and accumulation of agilawood substances, and compared with a traditional agilawood formation method, the agilawood formation method has the advantages that the agilawood formation rate is increased, and the agilawood formation quality is improved. According to the method, the agilawood with higher quality can be obtained within shorter time, and the yield is increased.

Description

Technical Field

[0001] The invention belongs to the technical field of fragrance formation of Aquilaria plants, and in particular relates to a Ganoderma lucidum fragrance formation promoter for promoting efficient fragrance formation of Aquilaria plants. Background Art

[0002] Agarwood comes from the dry resinous wood of the Aquilaria plant of the Thymelaeaceae family. It is named because it sinks in water and has a fragrant smell. The chemical components of agarwood mainly include sesquiterpenoids, 2-(2-phenylethyl) chromone, triterpenoids, aromatic compounds and other components. Among them, sesquiterpenoids and 2-(2-phenylethyl) chromone are the characteristic components of agarwood. Aquilaria sinensis does not produce agarwood under natural growth conditions. Only when it is subjected to external stresses such as lightning strikes, insect bites, fire and fungal infection, the tree's defense system is activated, and then an oily resin rich in volatile organic compounds is formed, thereby hindering fungal growth and starting a damage repair mechanism. In this process, secondary metabolites of agarwood accumulate and agarwood is produced.

[0003] The artificial agarwood formation methods include physical injury method, chemical stimulation method and comprehensive method. The cutting method in the physical injury method is to use a knife to cut a wound of a certain depth and length on the trunk of the white agarwood tree. After the wound is exposed, the tree body activates the defense mechanism to secrete resin; the drilling method is to use a chisel to drill holes in the trunk. After drilling the holes, inducers can be placed and sealed to destroy the internal structure of the trunk to promote the secretion of resin. The fungal inoculation method of the chemical stimulation method is to screen fungal strains to make bacterial liquid or bacterial agents, and inoculate them on the trunk or branches by injection, smearing or soaking; the chemical reagent method is to spray or inject ethephon, methyl jasmonate, etc. into a solution to regulate the physiological metabolism of the tree and promote resin synthesis. The physical and chemical combination method of the comprehensive method is to first physically injure such as cutting or drilling, then apply chemical inducers or inject chemical reagents, and then cut and treat. There is a problem of easy decomposition of plant hormones in the chemical stimulation method and the comprehensive method, which easily leads to unstable effects and short duration. For example, when using chemical reagents such as ethephon and methyl jasmonate, they are easily affected by factors such as light, heat, and microorganisms in the external environment and quickly decompose or inactivate, making it difficult to effectively regulate the physiological metabolism of the tree and promote resin synthesis for a long time. In the fungal inoculation method, although the selected fungal strains can stimulate the tree to produce fragrance to a certain extent, the growth and function of the fungi are also easily disturbed by the external environment, and their stability and controllability are poor. Summary of the invention

[0004] In view of the above situation, in order to overcome the defects of the prior art, the present invention disperses the plant hormones in a modified emulsion, protects the plant hormones, and synergizes with components such as microorganisms to achieve the purpose of efficient agarwood production of Aquilaria plants.

[0005] In order to achieve the above-mentioned purpose, the following technical scheme is adopted: the present invention provides a Ganoderma lucidum fragrance-forming promoter for promoting the efficient fragrance formation of Aquilaria plants, comprising component A and component B; component A comprises the following components in parts by weight: 20-40 parts of Aspergillus niger microbial fermentation broth, 15-30 parts of Verticillium foetidum microbial fermentation broth, 10-15 parts of Fusarium oxysporum microbial fermentation broth, 5-10 parts of Ganoderma lucidum extract, 6-8 parts of seaweed extract, 2-8 parts of malic acid, 3-6 parts of fructose, 1-3 parts of pectinase and 0.5-2 parts of cellulase.

[0006] Furthermore, the B component is a plant hormone xylan emulsion, which is prepared by the following steps:

[0007] S1. Add xylan to 2 mol / L sodium tartarate solution, slowly add 5 mol / L hydrochloric acid solution, hydrolyze at 50°C for 8 hours, then centrifuge to separate the suspension, and freeze-dry the suspension for 12 hours to obtain carboxylated xylan;

[0008] S2, adding the carboxylated xylan to DMF, then adding N,N-carbonyldiimidazole and 1,2-tetradecanoylphosphatidylethanolamine, heating to solvent reflux for 3 hours, then returning to room temperature, concentrating, and drying the precipitated solid to obtain carboxylated grafted xylan;

[0009] S3, dissolving the carboxylated grafted xylan in deionized water to obtain an aqueous solution, then subjecting the aqueous solution to ultrasonic treatment, adding the plant hormone to the oil phase to obtain an oil phase solution, dropping the oil phase solution into the aqueous solution, and homogenizing the resulting mixed solution by high-speed shearing to obtain a plant hormone xylan emulsion.

[0010] Furthermore, in step S1, the mass ratio of xylan, sodium tartarate solution and hydrochloric acid solution is 1:3-5:0.5-2.

[0011] Furthermore, in step S2, the mass ratio of carboxylated xylan, DMF, N,N-carbonyldiimidazole and 1,2-tetradecanoylphosphatidylethanolamine is 1:3-6:0.5-2:0.3-0.5.

[0012] Furthermore, the mass ratio of the carboxylated grafted xylan solution to deionized water in the aqueous phase solution is 1:10-20.

[0013] Furthermore, the mass ratio of the plant hormone to the oil phase in the oil phase solution is 1:5-10.

[0014] Furthermore, the oil phase is one or more of corn oil, soybean oil, hydrogenated castor oil, hydrogenated soybean oil and triglyceride.

[0015] Furthermore, the oil phase is preferably a combination of soybean oil and triglycerides.

[0016] Furthermore, in step S3, the mass ratio of carboxylated grafted xylan to plant hormone is 8-15:1.

[0017] Furthermore, the plant hormone is one or more of gibberellins, brassinosteroids, methyl jasmonate and 6-benzyladenine.

[0018] Furthermore, the plant hormone is preferably a combination of gibberellins, brassinosteroids and methyl jasmonate.

[0019] Furthermore, the Aspergillus niger microbial fermentation broth, the Verticillium dahliae microbial fermentation broth and the Fusarium oxysporum microbial fermentation broth are prepared by the following steps: Aspergillus niger, Verticillium dahliae and Fusarium oxysporum are inoculated into PDB culture medium respectively, Aspergillus niger is cultured at a temperature of 30° C., and Verticillium dahliae and Fusarium oxysporum are cultured at a temperature of 28° C. in a shaking incubator at a rotation speed of 200 rpm for 3 days, and the corresponding fermentation broth is obtained by filtration.

[0020] Furthermore, when the fragrance-forming promoter is used: select a white agarwood tree of at least 5 years old, first artificially make a wound on the trunk, the wound length is 3-8 cm, and the width is 1-3 mm, apply component A to the wound on the trunk, the coating thickness is 0.5-2 mm, then apply component B, the coating thickness is 0.5-2 mm, and then cover it with plastic wrap to maintain humidity and promote absorption.

[0021] The beneficial effects of the present invention are:

[0022] (1) The present invention effectively improves the agarwood formation rate and quality through the synergistic effect of component A and component B. The synergistic effect of microorganisms stimulates the agarwood formation potential of plants. The precise regulation of plant hormone xylan emulsion provides conditions for the synthesis and accumulation of agarwood substances. Compared with the traditional agarwood formation method, the present invention can obtain higher quality agarwood in a shorter time and improve the yield;

[0023] (2) The present invention modifies xylan to obtain carboxylated grafted xylan with better stability and functionality, increases the hydrophilicity and biocompatibility of its molecules, enables it to better interact with plant tissues, improves the absorption and utilization efficiency of plant hormones by plants, and can better combine with plant hormones to form a stable emulsion system, effectively encapsulate and protect plant hormones, and prevent them from being decomposed or inactivated too quickly in the external environment. The modification promotes the amphiphilic balance of the xylan molecular chain, so that it can be well dispersed in water to form a uniform aqueous solution, and at the same time can be stably adsorbed on the oil-water interface to form a barrier film, effectively protecting plant hormones from oxidative degradation, prolonging the effective action time of plant hormones, and blocking the influence of ultraviolet rays and external adverse conditions on microorganisms, providing a relatively stable microenvironment for the survival and action of microorganisms in plants.

[0024] (3) The present invention provides a variety of induction pathways for the formation of fragrance in Aquilaria plants through the synergistic effects of various microorganisms and organic and inorganic components, which can more comprehensively stimulate the physiological responses of plants and improve the efficiency and quality of fragrance formation; the microbial fermentation broth contains rich metabolites, and components such as malic acid and fructose can provide plants with additional energy sources and promote plant growth and metabolic activities. Pectinase and cellulase can decompose the components of plant cell walls and participate in the adjustment of plant cell structure, creating favorable conditions for the fragrance formation process. The Ganoderma lucidum extract contains a variety of biologically active ingredients that can regulate plant growth and enhance plant stress resistance, further synergizing with the microbial fermentation broth to promote the healthy growth of plants. The seaweed extract is rich in a variety of minerals, vitamins and growth regulators, which can enhance the photosynthesis and stress resistance of plants and improve the adaptability of plants to the environment, thereby facilitating the smooth progress of the fragrance formation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The results of the determination of the fragrance formation speed of Test Example 2 of the present invention are as follows;

[0026] Figure 2 The emulsion stability evaluation results of Test Example 3 of the present invention are as follows;

[0027] Figure 3 This is the microscopic morphology image of the emulsion of Test Example 4 of the present invention.

[0028] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0030] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0031] The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental materials used in the following examples are purchased from commercial channels unless otherwise specified.

[0032] Among them: Aspergillus niger, strain number: 3.15297, purchased from China General Microbiological Culture Collection Management Center.

[0033] Verticillium albo, strain number: CGMCC3.2869, was purchased from Shanghai Collection Biotechnology Center.

[0034] Fusarium oxysporum f.sp.spinaciae, product number: BMZ135222, was purchased from Ningbo Mingzhou Biotechnology Co., Ltd.

[0035] Ganoderma lucidum extract was purchased from Gansu Yishengxiang Biotechnology Co., Ltd.; seaweed extract was purchased from Chengdu Pusi Biotechnology Co., Ltd.; malic acid, fructose, pectinase, cellulase, xylan, sodium tartrate, hydrochloric acid, DMF and N,N-carbonyldiimidazole were purchased from Shanghai MacLean Biochemical Technology Co., Ltd.; 1,2-tetradecanoylphosphatidylethanolamine was purchased from Wuhan Kemik Biomedical Technology Co., Ltd.; gibberellin, brassinosteroids, methyl jasmonate and 6-benzyladenine were purchased from Jiangsu Caiwei Biotechnology Co., Ltd.

[0036] Example 1

[0037] A kind of Ganoderma lucidum aroma-forming promoter for promoting efficient aroma-forming of Aquilaria plants

[0038] The aroma-forming promoter comprises component A and component B; component A comprises the following components in parts by weight: 20 parts of Aspergillus niger microbial fermentation liquid, 15 parts of Verticillium foetidum microbial fermentation liquid, 10 parts of Fusarium oxysporum microbial fermentation liquid, 5 parts of Ganoderma lucidum extract, 6 parts of seaweed extract, 2 parts of malic acid, 3 parts of fructose, 1 part of pectinase and 0.5 parts of cellulase.

[0039] The B component is a plant hormone xylan emulsion, which is prepared by the following steps:

[0040] S1. Add xylan to 2 mol / L sodium tartarate solution, slowly add 5 mol / L hydrochloric acid solution, hydrolyze at 50°C for 8 hours, then centrifuge to separate the suspension, and freeze-dry the suspension for 12 hours to obtain carboxylated xylan;

[0041] S2, adding the carboxylated xylan to DMF, then adding N,N-carbonyldiimidazole and 1,2-tetradecanoylphosphatidylethanolamine, heating to solvent reflux for 3 hours, then returning to room temperature, concentrating, and drying the precipitated solid to obtain carboxylated grafted xylan;

[0042] S3, dissolving the carboxylated grafted xylan in deionized water to obtain an aqueous solution, then subjecting the aqueous solution to ultrasonic treatment, adding the plant hormone to the oil phase to obtain an oil phase solution, dropping the oil phase solution into the aqueous solution, and homogenizing the resulting mixed solution by high-speed shearing to obtain a plant hormone xylan emulsion.

[0043] In the step S1, the mass ratio of xylan, sodium tartrate solution and hydrochloric acid solution is 1:3:0.5; in the step S2, the mass ratio of carboxylated xylan, DMF, N,N-carbonyldiimidazole and 1,2-tetradecanoylphosphatidylethanolamine is 1:3:0.5:0.3; the mass ratio of carboxylated grafted xylan solution and deionized water in the aqueous phase solution is 1:10; the mass ratio of plant hormones to the oil phase in the oil phase solution is 1:5; the oil phase is a combination of corn oil and triglycerides in a mass ratio of 2:1; in the step S3, the mass ratio of carboxylated grafted xylan to plant hormones is 8:1; the plant hormones are a combination of gibberellins, brassinosteroids and 6-benzyladenine in a mass ratio of 3:1:2.

[0044] The Aspergillus niger microbial fermentation liquid, the Verticillium dahliae microbial fermentation liquid and the Fusarium oxysporum microbial fermentation liquid are prepared by the following steps: Aspergillus niger, Verticillium dahliae and Fusarium oxysporum are inoculated into PDB culture medium respectively, Aspergillus niger is cultured at a temperature of 30°C, and Verticillium dahliae and Fusarium oxysporum are cultured at a temperature of 28°C in a shaking incubator with a rotation speed of 200 rpm for 3 days, and the corresponding fermentation liquid is obtained by filtering.

[0045] Example 2

[0046] A kind of Ganoderma lucidum aroma-forming promoter for promoting efficient aroma-forming of Aquilaria plants

[0047] The aroma-forming promoter comprises component A and component B; component A comprises the following components in parts by weight: 40 parts of Aspergillus niger microbial fermentation liquid, 30 parts of Verticillium foetidum microbial fermentation liquid, 15 parts of Fusarium oxysporum microbial fermentation liquid, 10 parts of Ganoderma lucidum extract, 8 parts of seaweed extract, 8 parts of malic acid, 6 parts of fructose, 3 parts of pectinase and 2 parts of cellulase.

[0048] The B component is a plant hormone xylan emulsion, and the preparation process is the same as that of Example 1.

[0049] In the step S1, the mass ratio of xylan, sodium tartrate solution and hydrochloric acid solution is 1:5:2; in the step S2, the mass ratio of carboxylated xylan, DMF, N,N-carbonyldiimidazole and 1,2-tetradecanoylphosphatidylethanolamine is 1:6:2:0.5; the mass ratio of carboxylated grafted xylan solution and deionized water in the aqueous phase solution is 1:20; the mass ratio of plant hormone to oil phase in the oil phase solution is 1:10; the oil phase is a combination of hydrogenated castor oil, hydrogenated soybean oil and triglyceride in a mass ratio of 2:1:2; in the step S3, the mass ratio of carboxylated grafted xylan to plant hormone is 15:1; the plant hormone is a combination of brassinosteroids, methyl jasmonate and 6-benzyladenine in a mass ratio of 3:1:2.

[0050] The preparation process of the Aspergillus niger microbial fermentation liquid, the Verticillium foetidum microbial fermentation liquid and the Fusarium oxysporum microbial fermentation liquid is the same as that in Example 1.

[0051] Example 3

[0052] The aroma-forming promoter comprises component A and component B; component A comprises the following components in parts by weight: 30 parts of Aspergillus niger microbial fermentation liquid, 25 parts of Verticillium dahliae microbial fermentation liquid, 12 parts of Fusarium oxysporum microbial fermentation liquid, 8 parts of Ganoderma lucidum extract, 7 parts of seaweed extract, 6 parts of malic acid, 4.5 parts of fructose, 2 parts of pectinase and 1.2 parts of cellulase.

[0053] The B component is a plant hormone xylan emulsion, and the preparation process is the same as that of Example 1.

[0054] In step S1, the mass ratio of xylan, sodium tartarate solution and hydrochloric acid solution is 1:4:1.5.

[0055] The mass ratio of carboxylated xylan, DMF, N,N-carbonyldiimidazole and 1,2-tetradecanoylphosphatidylethanolamine in step S2 is 1:5:1:0.4; the mass ratio of carboxylated grafted xylan solution and deionized water in the aqueous phase solution is 1:15; the mass ratio of plant hormones to oil phase in the oil phase solution is 1:7; the oil phase is a combination of soybean oil and triglycerides in a mass ratio of 1:2; the mass ratio of carboxylated grafted xylan to plant hormones in step S3 is 12:1; the plant hormones are a combination of gibberellins, brassinosteroids and methyl jasmonate in a mass ratio of 2:1:3.

[0056] The preparation process of the Aspergillus niger microbial fermentation liquid, the Verticillium foetidum microbial fermentation liquid and the Fusarium oxysporum microbial fermentation liquid is the same as that in Example 1.

[0057] Comparative Example 1

[0058] In this comparative example, unmodified xylan was used instead of carboxylated grafted xylan, and the rest was the same as in Example 1.

[0059] Comparative Example 2

[0060] In this comparative example, the plant hormones were not protected by the emulsion, that is, the plant hormones were added to component A at 5% of the mass of component A, and the rest were the same as in Example 1.

[0061] Results Analysis

[0062] Test Example 1

[0063] Determination of extracts and active ingredients of agarwood essential oil

[0064] In this test example, the agarwood tree was treated with the agarwood formation promoters of Examples 1-3 and Comparative Examples 1-2 and traditional infusion fees, and the agarwood essential oil extract and the active ingredient content were determined after the agarwood formation.

[0065] The control group used the traditional infusion method to inject the bacterial solution into the wood of agarwood to produce agarwood. The bacterial solution used had the same composition as component A in Example 3.

[0066] The specific steps are as follows:

[0067] Select a white agarwood tree that is at least 5 years old, first make a wound on the trunk manually, the wound length is 3-8cm, the width is 1-3mm, apply component A to the wound on the trunk, the coating thickness is 0.5-2mm, then apply component B, the coating thickness is 0.5-2mm, and then cover it with plastic wrap to maintain humidity and promote absorption;

[0068] After 12 months, the brown oily substance and yellowish-brown discolored wood formed around the wound are cut off and dried to obtain agarwood.

[0069] The agarwood essential oil was extracted according to the volatile oil extraction method recorded in the 2020 edition of the "Pharmacopoeia of the People's Republic of China". The content of the ethanol extract and the relative contents of chromones, sesquiterpenes and agarwood tetraols in the agarwood volatile oil were determined. The results are shown in Table 1.

[0070] Table 1 Determination results of agarwood essential oil extract and active ingredient content

[0071] project Ethanol extract Relative content of chromone Sesquiterpenoid relative content Relative content of agarwood tetraol Example 1 52.55％ 25.53％ 62.50％ 0.87％ Example 2 55.82％ 27.14％ 58.77％ 0.95％ Example 3 56.72％ 24.63％ 63.42％ 1.05％ Comparative Example 1 30.58％ 18.75％ 48.43％ 0.42％ Comparative Example 2 27.25％ 16.55％ 40.33％ 0.33％ Traditional infusion set 21.68％ 14.38％ 38.75％ 0.15％

[0072] From the results in the above table, it can be seen that after the aroma agent prepared in Examples 1-3 treated the white agarwood tree for 12 months, the ethanol extract content, the relative content of chromones, the relative content of sesquiterpenes and the relative content of agarwood tetraols of the agarwood essential oil were significantly higher than those of the comparative examples 1-2 and the traditional infusion group, indicating that the aroma agent of the present invention can effectively increase the content of each effective ingredient in agarwood oil and agarwood oil, thereby producing high-quality agarwood. By comparing different embodiments and the control group, it can be seen that the aroma agent prepared by the present invention has obvious advantages in the content of each effective ingredient after treating the white agarwood tree, reflecting its significant effect in promoting the aroma formation of agarwood and improving the quality.

[0073] Test Example 2

[0074] Determination of fragrance formation speed

[0075] During the process of forming incense in Example 1, samples were taken from the wounds of the Aquilaria sinensis trees in Example 3, Comparative Example 1, Comparative Example 2 and the traditional infusion group every 3 months according to the method of Test Example 1, and then the content of the ethanol extract was tested. The results are shown in Table 1. Figure 1 .

[0076] Depend on Figure 1 It can be seen that within 0-3 months, the ethanol extract content in Example 3 increased significantly, and the fragrance formation speed was the fastest, and then the growth rate slowed down, while the ethanol extract content of Comparative Example 1, Comparative Example 2 and the traditional infusion group did not increase significantly, and the growth rate was relatively uniform, indicating that the fragrance agent of the present invention can quickly promote the fragrance formation of agarwood in a relatively short period of time and improve the fragrance formation efficiency.

[0077] Test Example 3

[0078] Emulsion stability assessment

[0079] The emulsion solution of Example 3, Comparative Example 1 and the control group (the oil phase solution with the same components as in Example 3) was heated at 15W / m 2 The emulsion was continuously irradiated under ultraviolet light for 240 hours, and the retention rate of plant hormones was tested every 40 hours to evaluate the stability of the emulsion. The results are shown in Figure 2 .

[0080] Depend on Figure 2It can be seen that the emulsion of Example 3 has a higher plant hormone retention rate under ultraviolet irradiation, indicating that the emulsion system formed after the xylan modification treatment of the present invention has good stability, can effectively encapsulate and protect plant hormones, prevent them from being decomposed or inactivated too quickly in the external environment, and prolong the effective action time of plant hormones. This stability is conducive to the continuous absorption and utilization of plant hormones by plants, provides stable conditions for the agarwood formation process, and further reflects the advantages of the present invention in improving the quality of agarwood formation.

[0081] Test Example 4

[0082] Emulsion microstructure observation

[0083] 50 μL of the emulsion droplets of Examples 1-3 and Comparative Example 1 were placed on a glass slide, slowly covered with a cover glass, and observed using an IX73 fluorescence inverted microscope system with an eyepiece magnification of 10X and an objective lens magnification of 40X under bright field conditions. The results are shown in Figure 3 .

[0084] Depend on Figure 3 It can be seen that in the emulsion prepared by the present invention, the spherical oil phase is evenly distributed in the liquid phase, and the droplet size is uniform, while the particle size difference in Example 1 is relatively large, which proves that the distribution of the oil phase in the emulsion is uneven, indicating that the emulsion system of the present invention has a better microstructure and can provide a more stable environment for the formation of agarwood. The uniform droplet size distribution helps to improve the stability and functionality of the emulsion, so that the plant hormones can act more evenly on the plant tissues and promote the formation of agarwood.

[0085] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0086] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A Ganoderma lucidum fragrance-forming promoter for promoting efficient fragrance formation of Aquilaria plants, characterized in that: The invention comprises component A and component B; component A comprises the following components in parts by weight: 20-40 parts of Aspergillus niger microbial fermentation liquid, 15-30 parts of Verticillium foetidum microbial fermentation liquid, 10-15 parts of Fusarium oxysporum microbial fermentation liquid, 5-10 parts of Ganoderma lucidum extract, 6-8 parts of seaweed extract, 2-8 parts of malic acid, 3-6 parts of fructose, 1-3 parts of pectinase and 0.5-2 parts of cellulase; The B component is a plant hormone xylan emulsion, which is prepared by the following steps: S1. Add xylan to 2 mol / L sodium tartarate solution, slowly add 5 mol / L hydrochloric acid solution, hydrolyze at 50°C for 8 hours, then centrifuge to separate the suspension, and freeze-dry the suspension for 12 hours to obtain carboxylated xylan; S2, adding the carboxylated xylan to DMF, then adding N,N-carbonyldiimidazole and 1,2-tetradecanoylphosphatidylethanolamine, heating to solvent reflux for 3 hours, then returning to room temperature, concentrating, and drying the precipitated solid to obtain carboxylated grafted xylan; S3, dissolving the carboxylated grafted xylan in deionized water to obtain an aqueous solution, then subjecting the aqueous solution to ultrasonic treatment, adding the plant hormone to the oil phase to obtain an oil phase solution, dropping the oil phase solution into the aqueous solution, and homogenizing the resulting mixed solution by high-speed shearing to obtain a plant hormone xylan emulsion.

2. The Ganoderma lucidum fragrance-forming promoter for promoting efficient fragrance-forming of Aquilaria plants according to claim 1, characterized in that: In step S1, the mass ratio of xylan, sodium tartarate solution and hydrochloric acid solution is 1:3-5:0.5-2.

3. The Ganoderma lucidum fragrance-forming promoter for promoting efficient fragrance-forming of Aquilaria plants according to claim 2, characterized in that: In the step S2, the mass ratio of carboxylated xylan, DMF, N,N-carbonyldiimidazole and 1,2-tetradecanoylphosphatidylethanolamine is 1:3-6:0.5-2:0.3-0.

5.

4. The Ganoderma lucidum fragrance-forming promoter for promoting efficient fragrance-forming of Aquilaria plants according to claim 3, characterized in that: The mass ratio of the carboxylated grafted xylan solution to deionized water in the aqueous phase solution is 1:10-20.

5. The Ganoderma lucidum fragrance-forming promoter for promoting efficient fragrance-forming of Aquilaria plants according to claim 4, characterized in that: The mass ratio of the plant hormone to the oil phase in the oil phase solution is 1:5-10.

6. The Ganoderma lucidum fragrance-forming promoter for promoting efficient fragrance-forming of Aquilaria plants according to claim 5, characterized in that: The oil phase is one or more of corn oil, soybean oil, hydrogenated castor oil, hydrogenated soybean oil and triglyceride.

7. The Ganoderma lucidum fragrance-forming promoter for promoting efficient fragrance-forming of Aquilaria plants according to claim 6, characterized in that: In the step S3, the mass ratio of carboxylated grafted xylan to plant hormone is 8-15:

1.

8. The Ganoderma lucidum fragrance-forming promoter for promoting efficient fragrance-forming of Aquilaria plants according to claim 7, characterized in that: The plant hormone is one or more of gibberellins, brassinosteroids, methyl jasmonate and 6-benzyladenine.

9. The Ganoderma lucidum fragrance-forming promoter for promoting efficient fragrance-forming of Aquilaria plants according to claim 8, characterized in that: The Aspergillus niger microbial fermentation liquid, the Verticillium dahliae microbial fermentation liquid and the Fusarium oxysporum microbial fermentation liquid are prepared by the following steps: Aspergillus niger, Verticillium dahliae and Fusarium oxysporum are inoculated into PDB culture medium respectively, Aspergillus niger is cultured at a temperature of 30°C, and Verticillium dahliae and Fusarium oxysporum are cultured at a temperature of 28°C in a shaking incubator with a rotation speed of 200 rpm for 3 days, and the corresponding fermentation liquid is obtained by filtering.

10. The Ganoderma lucidum fragrance-forming promoter for promoting efficient fragrance-forming of Aquilaria plants according to claim 9, characterized in that: When the fragrance-forming promoter is used: select a white agarwood tree with a tree age of at least 5 years, first artificially make a wound on the trunk, the wound length is 3-8 cm, and the width is 1-3 mm, apply component A to the wound on the trunk, the coating thickness is 0.5-2 mm, then apply component B, the coating thickness is 0.5-2 mm, and then cover it with plastic wrap to maintain humidity and promote absorption.

Images