Seedling cultivation method for improving growth rate and agarwood formation effect of agarwood and its application

Abstract

The present invention provides a method for raising seedlings and its application for improving the growth rate and fragrance effect of agarwood, and the method comprises the following steps: Step 1: Root promotion: soaking the cuttings in rooting powder solution A, Chinese medicine culture solution, and fungicide solution in sequence to obtain the treated cuttings; Step 2: Cutting: inserting the treated cuttings into the seedling medium and watering the rooting water once; Step 3: Strengthening the roots and seedlings: In the first 2 weeks after cutting, the root and seedlings are sprayed with a mass ratio of 4-6:1 using a plant nutrient solution and a rooting powder solution B, sprayed once every 3-4d, and then sprayed with a plant nutrient solution once a week, and disinfected and watered regularly, and after the seedling height grows to ≥40cm, agarwood cuttings are obtained, and the agarwood is cultivated in the field. The survival rate of the seedlings cultivated by the method is higher than 93%, and the fragrance rate of agarwood is increased to 100% within five years, and it can be harvested in five years.

Description

Technical Field

[0001] The invention belongs to the technical field of cultivation, and in particular relates to a seedling raising method for improving the growth rate and agarwood formation effect of agarwood and an application thereof. Background Art

[0002] Aquilaria sinensis, scientifically known as Aquilaria sinensis, is an evergreen tree of the genus Aquilaria in the family Thymelaeaceae. The preciousness of aquilaria sinensis lies in its ability to produce aquilaria. The growth cycle of aquilaria sinensis is relatively long, and it generally takes decades or even hundreds of years to mature and produce aquilaria. Due to the rarity and high value of aquilaria sinensis, wild aquilaria sinensis trees are facing a serious threat of illegal logging, resulting in the increasing depletion of their wild resources.

[0003] Agarwood is easy to induce rooting, so cuttings are usually used for propagation in agarwood seedling production. Different cutting seedling methods lead to large differences in survival rates after seedling and transplanting. At present, the commonly used substrate for cutting seedling production is local, but due to the different sources of local soil, the soil type, soil organic matter content and soil porosity are different. There are a series of differences in the survival rate of different local seedlings, and local seedlings have problems such as low survival rate, slow root growth, weak growth potential, and slow agarwood formation.

[0004] In the prior art, the use of Chinese medicine ingredients for seedling cultivation has attracted more and more attention. The literature reports that Wang Dongsheng et al. made a seedling matrix using decomposed Chinese medicine residues, decomposed mushroom residues, peat, vermiculite, and perlite as raw materials, which significantly improved the plant height, stem thickness, root length and surface area of ​​pepper growth; Wu Tao et al. used piled decomposed medicine residues and corn stalks to form a matrix to replace peat for tobacco floating seedling cultivation. The results showed that it could meet the standard requirements for strong seedlings, but it was not ideal. At present, Chinese medicine nutrient matrix fertilizer is mainly used. When using Chinese medicine nutrient matrix fertilizer, it is usually mixed into the seedling soil, or applied to the seedbed as a base fertilizer, and absorbed by the plant roots through the soil. It is used to provide a healthy growth environment for seedlings, promote root development, enhance plant resistance, and focus more on improving the soil environment. At present, however, it has not been found that Chinese medicine extracts are used in the seedling cultivation of agarwood.

[0005] Therefore, there is an urgent need for a seedling cultivation method that can improve the growth rate and agarwood formation effect of agarwood. Summary of the invention

[0006] The purpose of the present invention is to provide a seedling raising method and application thereof for improving the growth rate and agarwood formation effect of agarwood.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] The method for raising seedlings for improving the growth rate and agarwood formation effect of agarwood comprises the following steps:

[0009] Step 1: Root promotion: Select one-year-old healthy branches of Aquilaria sinensis from a 3-5-year-old healthy mother plant as cuttings, and soak the cuttings in rooting powder solution A, Chinese medicine cultivation solution, and fungicide solution in sequence to obtain treated cuttings;

[0010] The preparation method of the Chinese medicine cultivation solution is:

[0011] (1) Mix the roots of Scutellaria baicalensis Georgi, Rheum palmatum and Acanthopanax gracilistylus in a mass ratio of 1:1.3-1.6:0.5-0.8, grind them and pass through a 80-100 mesh sieve to obtain a mixed powder;

[0012] (2) mixing the mixed powder, water and Lactobacillus bran in a mass ratio of 1:10-13:0.1-0.2, performing anaerobic fermentation at a fermentation temperature of 35-40° C. and a fermentation time of 12-15 h, and sterilizing to obtain a first fermentation product;

[0013] (3) adding 0.2-0.3 times the mass of the mixed micropowder to the first fermentation product, performing anaerobic fermentation at a temperature of 32-35° C. for 10-15 h, and sterilizing to obtain a second fermentation product;

[0014] (4) adding 0.1-0.2 times the mass of the mixed micropowder Bifidobacterium longum to the second fermentation product for anaerobic fermentation at a temperature of 35-37° C. for 15-20 h, and sterilizing to obtain a third fermentation product;

[0015] (5) filtering the third fermentation product to obtain a filtrate, concentrating under reduced pressure at 60-70° C. to a relative density of 1.01-1.03, mixing the extract and water at a mass ratio of 3-7:500, stirring evenly, and obtaining a Chinese medicine culture solution;

[0016] Step 2: Cutting: inserting the treated cuttings into the seedling medium and watering them once for rooting; the cutting depth is the same as the thickness of the covering layer; the seedling medium includes a covering layer and a base layer from top to bottom;

[0017] Step 3: Strengthening roots and seedlings: In the first 2 weeks after cuttings, the roots and seedlings are strengthened by spraying a combination of plant nutrient solution and rooting powder solution B in a mass ratio of 4-6:1, spraying once every 3-4 days, and then spraying plant nutrient solution once a week, and disinfecting and watering regularly. When the seedling height grows to ≥40cm, the agarwood cuttings are obtained and cultivated in the field.

[0018] The Chinese medicine nutrient matrix fertilizer made of Chinese medicine ingredients is not ideal for promoting the growth rate of agarwood. The Chinese medicine ingredients may have antagonistic effects with other components in the fertilizer, affecting the use effect. The present invention selects a specific proportion of Scutellaria baicalensis, Rheum palmatum and Acanthopanax slenderii roots, and obtains a Chinese medicine cultivation liquid through triple fermentation of three strains. After the cuttings are soaked, the survival rate of the cultivated seedlings is higher than 93%. After the fermentation of Scutellaria baicalensis, Rheum palmatum and Acanthopanax slenderii roots, Scutellaria baicalensis contains flavonoid compounds, Rheum palmatum contains anthraquinone compounds, and Acanthopanax slenderii contains a variety of biologically active ingredients. The three strains introduced during the fermentation process can decompose complex plant components and convert them into a form that is more easily absorbed by plants, and at the same time produce plant growth promoting factors, such as plant growth hormones, vitamins and amino acids, which are beneficial to the growth and development of plants. At the same time, the plant immune system can be enhanced, and the active ingredients can enhance the defense mechanism of agarwood, so that the resistance of agarwood trees to diseases and insect pests is enhanced, and the growth stagnation or death caused by diseases is reduced, thereby indirectly increasing the growth rate. However, the agarwood yield rate is not ideal. It can only be harvested after more than five years of cultivation, and the agarwood yield rate within five years is less than 95%.

[0019] Furthermore, the preparation method of the plant nutrient solution is:

[0020] (1) Mixing Plantago, Curcuma, Stemona, Rhizoma Alpiniae, and Pericarpium Citri Reticulatae in a mass ratio of 1:1.3-1.5:1.6-1.9:0.3-0.7:0.4-0.8, crushing and passing through a 80-100 mesh sieve to obtain a mixture;

[0021] (2) subjecting the mixed material to supercritical extraction, first extracting at 45-50° C., 35-38 MPa, and a CO2 flow rate of 15-20 L / h for 60-80 min to obtain a first fraction, then further extracting at 35-40° C., 30-33 MPa, and a CO2 flow rate of 15-20 L / h for 40-50 min to obtain a second fraction; then extracting at 25-30° C., 20-23 MPa, and a CO2 flow rate of 15-20 L / h for 20-30 min to obtain a third fraction;

[0022] (3) mixing the first fraction, the second fraction and the third fraction to obtain a mixed extract;

[0023] (4) Mixing the mixed extract and water in a mass ratio of 1-3:500 to obtain a plant nutrient solution.

[0024] Further, the rooting powder solution B comprises, by mass, 2-4 parts of naphthaleneacetic acid, 1-3 parts of indolebutyric acid, 5-8 parts of cinnamic acid and 1000 parts of water;

[0025] The present invention uses a plant nutrient solution and a rooting powder solution B to spray agarwood to strengthen the roots and seedlings, so that the agarwood fragrance rate can be increased to 100% within five years, and the agarwood can be harvested in five years. After the plant nutrient solution and the rooting powder solution B are mixed, a synergistic effect is generated. Naphthaleneacetic acid and indolebutyric acid can promote the growth of plant roots, improve the rooting rate and root quality of cuttings, and enhance the root absorption capacity by stimulating cell division and elongation, thereby providing a better nutrient absorption pathway for agarwood trees and promoting their overall growth and health. Cinnamic acid: It has a broad spectrum of antibacterial and antifungal effects, and may also participate in plant signal transduction and affect the physiological activities of plants. The biologically active ingredients contained in plant extracts such as plantain, curcuma, radix stemonae, ginger, and tangerine peel can enhance the immunity and stress resistance of agarwood trees, promote healthy growth, and may also affect the growth cycle and fragrance formation process of agarwood trees by regulating the balance of plant hormones.

[0026] Furthermore, the cuttings are immersed in the Chinese medicinal cultivation solution for 25-30 hours.

[0027] Furthermore, the concentration of ABT rooting powder in the rooting powder solution A is 45-50 mg / L, and the cuttings are immersed in the rooting powder solution for 0.8-1.2 h.

[0028] Furthermore, the mass concentration of carbendazim in the fungicide solution is 0.1-0.2%, and the cuttings are immersed in the fungicide solution for 5-10 minutes.

[0029] Furthermore, the length of the cuttings is 15-20 cm, and 2-3 leaves are retained.

[0030] Furthermore, the thickness ratio of the covering layer to the base layer is 1:4-6.

[0031] Furthermore, the total thickness of the covering layer and the base layer is 20-30 cm.

[0032] Furthermore, the covering layer is mixed with loess, sludge and vermiculite in a mass ratio of 2-3:1:1-2.

[0033] Furthermore, the base layer is mixed with laterite, peat and perlite in a mass ratio of 1:3-5:2-3.

[0034] The invention also provides the application of the seedling raising method for improving the growth rate and agarwood formation effect of agarwood.

[0035] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0036] 1. The present invention provides a seedling cultivation method and application thereof for improving the growth rate and agarwood formation effect of agarwood. The survival rate of seedlings cultivated by the method is higher than 93%, the agarwood formation rate is increased to 100% within five years, and the agarwood can be harvested within five years.

[0037] 2. The present invention selects Scutellaria baicalensis, Rheum palmatum root and Acanthopanax scapularis root in a specific ratio, and obtains a Chinese medicine culture solution through triple fermentation of the three strains. After the cuttings are soaked in the solution, the survival rate of the cultivated seedlings is higher than 93%.

[0038] 3. The present invention uses a compound of plant nutrient solution and rooting powder solution B to spray agarwood to strengthen roots and seedlings, which can increase the agarwood yield rate to 100% within five years and can be harvested within five years. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] The strains used in the present invention are all commercially available, as follows:

[0041] Lactobacillus furfur, with the deposit number of JCM2762, is deposited in the Japan Culture Collection of Microorganisms.

[0042] Aspergillus niger, with the deposit number of CI CC 40048, is deposited in China Center for the Collection of Common Microbiological Cultures.

[0043] Bifidobacterium longum, with the deposit number of CCTCC HB 20082718, is deposited in China Center for Type Culture Collection.

[0044] Bifidobacterium lactis, with the deposit number of SHBCC D 24675, is deposited in Shanghai Collection Biotechnology Center.

[0045] Aspergillus oryzae, with the deposit number of CI CC 2066, is deposited in the China Center for the Collection of Common Microbiological Cultures.

[0046] Lactobacillus helveticus, with the deposit number of CCTCC AB 2010205, is deposited in China Center for Type Culture Collection.

[0047] Example 1

[0048] This embodiment provides a method for raising seedlings for improving the growth rate and agarwood formation effect of agarwood, comprising the following steps:

[0049] Step 1: Root promotion: Select one-year-old healthy branches of Aquilaria sinensis with a length of 17 cm from a 4-year-old healthy mother plant as cuttings, retain 3 leaves, and soak the cuttings in rooting powder solution A, Chinese medicine cultivation solution, and fungicide solution in sequence to obtain treated cuttings;

[0050] The rooting powder solution A is an aqueous solution with a concentration of 47 mg / L of ABT rooting powder, and the cuttings are immersed in the rooting powder solution A for 1 hour.

[0051] The fungicide solution is an aqueous solution with a mass concentration of 0.15% of carbendazim, and the cuttings are immersed in the fungicide solution for 8 minutes.

[0052] The preparation method of the Chinese medicine cultivation solution is:

[0053] (1) Mixing Scutellaria baicalensis, Rheum palmatum root and Acanthopanax gracilis root in a mass ratio of 1:1.4:0.7, crushing and passing through a 100-mesh sieve to obtain a mixed powder;

[0054] (2) mixing the mixed powder, water and Lactobacillus furfurali in a mass ratio of 1:12:0.15, performing anaerobic fermentation at a fermentation temperature of 37° C. and a fermentation time of 13 h, and sterilizing to obtain a first fermentation product;

[0055] (3) adding 0.2 times the mass of the mixed micropowder to the first fermentation product, and performing anaerobic fermentation at a temperature of 34° C. and a fermentation time of 13 h, and sterilizing to obtain a second fermentation product;

[0056] (4) adding 0.1 times the mass of the mixed micropowder Bifidobacterium longum to the second fermentation product for anaerobic fermentation at a temperature of 36° C. for 17 h, and sterilizing to obtain a third fermentation product;

[0057] (5) filtering the third fermentation product to obtain a filtrate, concentrating under reduced pressure at 65° C. to a relative density of 1.02, mixing the extract and water at a mass ratio of 7:500, stirring evenly, and obtaining a Chinese medicine culture solution;

[0058] Soak the cuttings in the Chinese medicinal culture solution for 27 hours.

[0059] Step 2: Cutting: inserting the treated cuttings into the seedling medium and watering them once for rooting; the cutting depth is the same as the thickness of the covering layer; the seedling medium includes a covering layer and a base layer from top to bottom;

[0060] The thickness ratio of the covering layer to the base layer is 1:5.

[0061] The total thickness of the cover layer and the base layer is 25 cm.

[0062] The covering layer is a mixture of loess, sludge and vermiculite in a mass ratio of 2:1:1.

[0063] The base layer is a mixture of red soil, peat and perlite in a mass ratio of 1:4:3.

[0064] Step 3: Strengthening roots and seedlings: In the first 2 weeks after cuttings, the roots and seedlings are strengthened by spraying a combination of plant nutrient solution and rooting powder solution B in a mass ratio of 5:1, spraying once every 4 days, and then spraying plant nutrient solution once a week, and disinfecting and watering regularly. When the seedling height grows to ≥40cm, the agarwood cuttings are obtained and cultivated in the field.

[0065] The preparation method of the plant nutrient solution is:

[0066] (1) Mixing Plantago asiatica, Curcuma aromatica, Stemona tuber, Rhizoma Alpiniae Rhizoma and Pericarpium Citri Reticulatae in a mass ratio of 1:1.4:1.7:0.5:0.6, crushing and passing through a 100-mesh sieve to obtain a mixture;

[0067] (2) subjecting the mixed material to supercritical extraction, firstly extracting at 47° C., 37 MPa, and a CO2 flow rate of 18 L / h for 70 min to obtain a first fraction, then further extracting at 37° C., 32 MPa, and a CO2 flow rate of 18 L / h for 45 min to obtain a second fraction; then extracting at 27° C., 22 MPa, and a CO2 flow rate of 18 L / h for 25 min to obtain a third fraction;

[0068] (3) mixing the first fraction, the second fraction and the third fraction to obtain a mixed extract;

[0069] (4) The mixed extract and water are mixed in a mass ratio of 1:500 to obtain a plant nutrient solution.

[0070] In parts by mass, the rooting powder solution B includes: 3 parts of naphthaleneacetic acid, 2 parts of indolebutyric acid, 6 parts of cinnamic acid and 1000 parts of water.

[0071] Example 2

[0072] This embodiment provides a method for raising seedlings for improving the growth rate and agarwood formation effect of agarwood, comprising the following steps:

[0073] Step 1: Root promotion: Select one-year-old healthy branches of Aquilaria sinensis with a length of 18 cm and two leaves from a three-year-old healthy mother plant as cuttings, and soak the cuttings in rooting powder solution A, Chinese medicine cultivation solution, and fungicide solution in sequence to obtain treated cuttings;

[0074] The rooting powder solution A is an aqueous solution of ABT rooting powder with a concentration of 45 mg / L, and the cuttings are immersed in the rooting powder solution A for 1.2 h.

[0075] The fungicide solution is an aqueous solution with a mass concentration of 0.1% of carbendazim, and the cuttings are immersed in the fungicide solution for 10 minutes.

[0076] The preparation method of the Chinese medicine cultivation solution is:

[0077] (1) Mixing Scutellaria baicalensis, Rheum palmatum root and Acanthopanax serrata root in a mass ratio of 1:1.3:0.8, crushing and passing through an 80-mesh sieve to obtain a mixed powder;

[0078] (2) mixing the mixed powder, water and Lactobacillus furfurali in a mass ratio of 1:10:0.1, performing anaerobic fermentation at a fermentation temperature of 35° C. and a fermentation time of 15 h, and sterilizing to obtain a first fermentation product;

[0079] (3) adding 0.2 times the mass of the mixed micropowder to the first fermentation product, and performing anaerobic fermentation at a temperature of 32° C. for 10 h, followed by sterilization to obtain a second fermentation product;

[0080] (4) adding 0.1 times the mass of the mixed micropowder Bifidobacterium longum to the second fermentation product for anaerobic fermentation at a temperature of 37° C. for 15 h, and sterilizing to obtain a third fermentation product;

[0081] (5) filtering the third fermentation product to obtain a filtrate, concentrating under reduced pressure at 70° C. to a relative density of 1.01, mixing the extract and water at a mass ratio of 7:500, stirring evenly, and obtaining a Chinese medicine culture solution;

[0082] Soak the cuttings in the Chinese medicinal culture solution for 25 hours.

[0083] Step 2: Cutting: inserting the treated cuttings into the seedling medium and watering them once for rooting; the cutting depth is the same as the thickness of the covering layer; the seedling medium includes a covering layer and a base layer from top to bottom;

[0084] The thickness ratio of the covering layer to the base layer is 1:6.

[0085] The total thickness of the cover layer and the base layer is 20 cm.

[0086] The covering layer is a mixture of loess, sludge and vermiculite in a mass ratio of 3:1:1.

[0087] The base layer is a mixture of red soil, peat and perlite in a mass ratio of 1:5:2.

[0088] Step 3: Strengthening roots and seedlings: In the first 2 weeks after cuttings, the roots and seedlings are strengthened by spraying a combination of plant nutrient solution and rooting powder solution B in a mass ratio of 6:1, spraying once every 3 days, and then spraying plant nutrient solution once a week, and disinfecting and watering regularly. When the seedling height grows to ≥40cm, the agarwood cuttings are obtained and cultivated in the field.

[0089] The preparation method of the plant nutrient solution is:

[0090] (1) Mixing Plantago asiatica, Curcuma aromatica, Stemona tuber, Rhizoma Alpiniae Rhizoma and Pericarpium Citri Reticulatae in a mass ratio of 1:1.3:1.9:0.3:0.8, crushing and passing through an 80-mesh sieve to obtain a mixture;

[0091] (2) subjecting the mixed material to supercritical extraction, firstly extracting at 50° C., 35 MPa, and a CO2 flow rate of 20 L / h for 80 min to obtain a first fraction, then further extracting at 35° C., 33 MPa, and a CO2 flow rate of 20 L / h for 50 min to obtain a second fraction; then extracting at 25° C., 23 MPa, and a CO2 flow rate of 15 L / h for 30 min to obtain a third fraction;

[0092] (3) mixing the first fraction, the second fraction and the third fraction to obtain a mixed extract;

[0093] (4) The mixed extract and water are mixed in a mass ratio of 3:500 to obtain a plant nutrient solution.

[0094] In parts by mass, the rooting powder solution B includes: 4 parts of naphthaleneacetic acid, 1 part of indolebutyric acid, 8 parts of cinnamic acid and 1000 parts of water.

[0095] Comparative Example 1

[0096] The difference between this comparative example and Example 1 is that the mass ratio of Scutellaria baicalensis, Rheum palmatum root and Acanthopanax gracilis root is 1:1:1.

[0097] Comparative Example 2

[0098] The difference between this comparative example and Example 1 is that the mixture of Scutellaria baicalensis, Rheum palmatum root and Acanthopanax scapularis root in a mass ratio of 1:1.4:0.7 is replaced by the mixture of Licorice, Portulaca oleracea and Cinnamomum cassia in a mass ratio of 1:1.4:0.7.

[0099] Comparative Example 3

[0100] The difference between this comparative example and Example 1 is that Lactobacillus granarium is replaced by Bifidobacterium lactis; Aspergillus niger is replaced by Aspergillus oryzae; and Bifidobacterium longum is replaced by Lactobacillus helveticus.

[0101] Comparative Example 4

[0102] The difference between this comparative example and Example 1 is that the mass ratio of Plantago seed, Curcuma aromatica, Stemona tuber, Alpinia zingiberensis and dried orange peel is 1:1:1:1:1.

[0103] Comparative Example 5

[0104] The difference between this comparative example and Example 1 is that the plantago seeds, curcuma, stemona, ginger and tangerine peel in a mass ratio of 1:1.4:1.7:0.5:0.6 are replaced by longan, polygonatum, polygonatum, schizonepeta and pepper in a mass ratio of 1:1.4:1.7:0.5:0.6.

[0105] Comparative Example 6

[0106] The difference between this comparative example and Example 1 is that the rooting powder solution B is an aqueous solution containing ABT rooting powder at a concentration of 47 mg / L.

[0107] Comparative Example 7

[0108] The difference between this comparative example and Example 1 is that the mass ratio of the plant nutrient solution and the rooting powder solution B for combined spraying is 1:1.

[0109] Comparative Example 8

[0110] The difference between this comparative example and Example 1 is:

[0111] The preparation method of the plant nutrient solution is:

[0112] (1) Mixing Plantago asiatica, Curcuma aromatica, Stemona tuber, Rhizoma Alpiniae Rhizoma and Pericarpium Citri Reticulatae in a mass ratio of 1:1.4:1.7:0.5:0.6, crushing and passing through a 100-mesh sieve to obtain a mixture;

[0113] (2) adding 65% ethanol aqueous solution in an amount of 10 times the mass of the mixture, heating and refluxing for 2 h, extracting three times, filtering, combining the extracts, filtering with a microfiltration membrane, and spray drying to obtain an extract;

[0114] (3) The extract and water are mixed in a mass ratio of 1:500 to obtain a plant nutrient solution.

[0115] Performance Testing

[0116] The robust agarwood mother plants grown in the same batch in the test nursery were selected, and 300 plants were treated according to the methods of the above-mentioned Examples 1-2 and Comparative Examples 1-8, and 3 parallel tests were set for each group.

[0117] (1) After 6 months of cuttings and seedling raising, the growth of each treated agarwood cutting was investigated, the rooting position, number of roots, and longest root length of the cuttings were recorded, and the rooting rate and survival rate were calculated. The results are shown in Table 1 below;

[0118] Average root number = total number of roots of all plants / number of plants;

[0119] Rooting rate = number of rooted plants / total number of plants × 100%;

[0120] Survival rate = number of surviving plants / total number of cuttings × 100%.

[0121] Except for the different seedling medium, other conditions were the same. The survival rate and root length of the cuttings were measured 60 days after cutting.

[0122] (2) The surviving seedlings were taken out of the nursery and planted in the field. 20 trees were randomly selected from each group of Examples 1-2 and Comparative Examples 1-8, planted, and each tree was marked and managed in the same way. The time was from April 14, 2018 to April 14, 2023. At the end of the fifth year, the number of fragrant agarwood plants in each group and the number of fragrant agarwood plants that could be harvested were counted.

[0123] Table 1 Test results

[0124]

[0125] It can be seen from the above performance test results that the survival rate and root growth effect of the agarwood cuttings of Examples 1-2 are good, especially the comprehensive performance of Example 1 is the most outstanding, which is mainly because the culture conditions such as the traditional Chinese medicine culture solution, plant nutrient solution and rooting powder solution used produce a synergistic effect.

[0126] However, the comparative examples did not adopt the necessary technical solutions, resulting in corresponding performance tests that were significantly worse than those of the embodiments. In comparative examples 1-4, the preparation conditions of the Chinese medicine cultivation solution were changed, and it can be seen that the survival rate and the root growth effect decreased, proving that the preparation conditions of the Chinese medicine cultivation solution have an important influence on the survival rate and the root growth effect. In comparative examples 4-8, the conditions of the plant nutrient solution and the rooting powder solution B were changed, and it can be seen from the results that the agarwood fragrance rate was affected. The above experimental results further prove the importance of the technical solution defined in the present invention for its technical effects.

[0127] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for raising seedlings for improving the growth rate and agarwood formation effect of agarwood, characterized in that: The following steps are involved: Step 1: Root promotion: Select one-year-old healthy branches of Aquilaria sinensis from a 3-5-year-old healthy mother plant as cuttings, and soak the cuttings in rooting powder solution A, Chinese medicine cultivation solution, and fungicide solution in sequence to obtain treated cuttings; The preparation method of the Chinese medicine cultivation solution is: (1) mixing Scutellaria baicalensis, Rheum palmatum root and Acanthopanax serrata root in a mass ratio of 1:1.3-1.6:0.5-0.8, crushing and passing through an 80-100 mesh sieve to obtain a mixed powder; (2) mixing the mixed powder, water and Lactobacillus bran in a mass ratio of 1:10-13:0.1-0.2, performing anaerobic fermentation at a fermentation temperature of 35-40° C. and a fermentation time of 12-15 h, and sterilizing to obtain a first fermentation product; (3) adding 0.2-0.3 times the mass of Aspergillus niger mixed micropowder to the first fermentation product for anaerobic fermentation at a temperature of 32-35° C. for 10-15 h, and sterilizing to obtain a second fermentation product; (4) adding 0.1-0.2 times the mass of the mixed micropowder Bifidobacterium longum to the second fermentation product for anaerobic fermentation at a temperature of 35-37° C. for 15-20 h, and sterilizing to obtain a third fermentation product; (5) filtering the third fermentation product to obtain a filtrate, concentrating under reduced pressure at 60-70° C. to a relative density of 1.01-1.03, mixing the extract and water at a mass ratio of 3-7:500, stirring evenly, and obtaining a Chinese medicine culture solution; Step 2: Cutting: inserting the treated cuttings into the seedling medium and watering them once for rooting; the cutting depth is the same as the thickness of the covering layer; the seedling medium includes a covering layer and a base layer from top to bottom; Step 3: Strengthening roots and seedlings: In the first 2 weeks after cutting, the root strengthening and seedling strengthening is carried out by spraying a combination of plant nutrient solution and rooting powder solution B in a mass ratio of 4-6:1, spraying once every 3-4 days, and then spraying plant nutrient solution once a week, and disinfecting and watering regularly. When the seedling height grows to ≥40cm, the agarwood cutting seedlings are obtained and cultivated in the field; The preparation method of the plant nutrient solution is: (1) Mixing Plantago, Curcuma, Stemona, Rhizoma Alpiniae, and Pericarpium Citri Reticulatae in a mass ratio of 1:1.3-1.5:1.6-1.9:0.3-0.7:0.4-0.8, crushing and passing through a 80-100 mesh sieve to obtain a mixture; (2) subjecting the mixed material to supercritical extraction, firstly extracting at 45-50° C., 35-38 MPa, and a CO2 flow rate of 15-20 L / h for 60-80 min to obtain a first fraction, then further extracting at 35-40° C., 30-33 MPa, and a CO2 flow rate of 15-20 L / h for 40-50 min to obtain a second fraction; then extracting at 25-30° C., 20-23 MPa, and a CO2 flow rate of 15-20 L / h for 20-30 min to obtain a third fraction; (3) mixing the first fraction, the second fraction and the third fraction to obtain a mixed extract; (4) mixing the mixed extract and water in a mass ratio of 1-3:500 to obtain a plant nutrient solution; In parts by mass, the rooting powder solution B comprises: 2-4 parts of naphthaleneacetic acid, 1-3 parts of indolebutyric acid, 5-8 parts of cinnamic acid and 1000 parts of water; The concentration of ABT rooting powder in the rooting powder solution A is 45-50 mg / L.

2. The method for raising seedlings for improving the growth rate and agarwood formation effect of agarwood according to claim 1, characterized in that: Soak the cuttings in the Chinese medicinal culture solution for 25-30 hours.

3. The method for raising seedlings for improving the growth rate and agarwood formation effect of claim 1, characterized in that: The cuttings are immersed in the rooting powder solution A for 0.8-1.2 hours.

4. The method for raising seedlings for improving the growth rate and agarwood formation effect of claim 1, characterized in that: The mass concentration of carbendazim in the fungicide solution is 0.1-0.2%, and the cuttings are immersed in the fungicide solution for 5-10 minutes.

5. The method for raising seedlings for improving the growth rate and agarwood formation effect of claim 1, characterized in that: The length of the cuttings is 15-20 cm, and 2-3 leaves are retained.

6. The method for raising seedlings for improving the growth rate and agarwood formation effect of agarwood according to claim 1, characterized in that: The covering layer is a mixture of loess, sludge and vermiculite in a mass ratio of 2-3:1:1-2.

7. The method for raising seedlings for improving the growth rate and agarwood formation effect of claim 1, characterized in that: The base layer is a mixture of red soil, peat and perlite in a mass ratio of 1:3-5:2-3.

8. Use of a seedling raising method for improving the growth rate and agarwood formation effect of agarwood as described in any one of claims 1 to 7.

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