Site-specific integration transgenosis method for Arabidopsis thaliana L.

A fixed-point integration and transgenic technology, which is applied in the field of fixed-point integration and transgenesis of Arabidopsis thaliana, can solve the problems of long experimental period and low transformation efficiency, and achieve the effect of short experimental period, high transformation efficiency and great practical application value
CN101892260AInactive Publication Date: 2010-11-24INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
Publication Date
2010-11-24
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a site-specific integration transgenosis method for Arabidopsis thaliana L., which comprises the following steps of: integrating target sites into genomes of target plants with agrobacterium rhizogenes by utilizing a florescence spraying method, and screening single-copy heterozygote transgenic plants out; and integrating target genes into the genomes of the screened single-copy heterozygote transgenic plants with the agrobacterium rhizogenes by utilizing the florescence spraying method again, and screening specific-site integrated homozygote plants out as successfully specific-site integrated plants. The site-specific integration transgenosis method for the Arabidopsis thaliana L. has the advantages of high conversion rate and short cycle.
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Claims

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