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High-density continuous culture method of nannochloropsis

A technique of Nannochloropsis and a cultivation method, which is applied in the field of high-density continuous cultivation of Nannochloropsis, can solve problems such as inability to carry out continuous cultivation work, and achieve the effect of highlighting the use value and ensuring the cultivation efficiency.

Inactive Publication Date: 2020-07-10
ZHANJIANG GUOLIAN AQUATIC PROD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a high-density continuous culture method for Nannochloropsis to solve the problem that most of the current Nannochloropsis culture methods proposed by the above-mentioned background technology cannot carry out continuous culture work

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The invention provides a technical solution: a method for continuously cultivating Nannochloropsis at high density, comprising the following steps:

[0020] A. Inoculation step: configure the medium in a closed photoreactor, then use an ultraviolet lamp to sterilize for 30 minutes, inoculate the Nannochloropsis grown to the exponential growth phase into the above-mentioned medium, and the inoculum amount of the Nannochloropsis culture solution is 5-20%;

[0021] B. Cultivation method: artificial LED light source is used for cultivation, and the cultivation steps are divided into two processes in turn. Initial process: the culture temperature is 20-30°C, irradiated with monochromatic white light for 3-5 days, and the intensity of white light is 500-2000lx. Continuous cultivation process: on the fifth day of cultivation, release half of the culture medium, add half of the fresh medium to continue fermentation, and then release half of the volume every 2 days as Nannoch...

Embodiment 2

[0030] A high-density continuous culture method for Nannochloropsis, comprising the following steps:

[0031] A. Inoculation steps: prepare medium in a closed photoreactor, each liter of medium contains: 0.5-3 grams of urea, 0.3-1 gram of sodium nitrate, 0.02-0.1 grams of potassium dihydrogen phosphate, 0.05-0.2 grams of magnesium sulfate, 0.03-0.2 grams of calcium chloride, 0.01-0.1 grams of ferrous sulfate, 0.01-0.1 grams of NA-EDTA, 1 ml of trace element mother liquor, each liter of trace element mother liquor contains: 0.01-0.03 grams of copper sulfate pentahydrate, 0.01- 0.05 grams of zinc sulfate heptahydrate, 0.01 to 0.02 grams of cobalt chloride hexahydrate, 0.1 to 0.3 grams of manganese chloride tetrahydrate, 0.01 to 0.1 grams of sodium molybdate dihydrate, 1 ml of vitamin mother solution, per liter of vitamin mother solution Contains: 0.1 to 1 gram of vitamin B1, 0.1 to 1 gram of vitamin B6, 0.1 to 1 gram of vitamin B12, and 0.1 to 1 gram of biotin. Disinfect for 3...

Embodiment 3

[0034] A high-density continuous culture method for Nannochloropsis, comprising the following steps:

[0035] A. Inoculation steps: configure the medium in a closed photoreactor, and each liter of the medium contains: 0.5 grams of urea, 0.3 grams of sodium nitrate, 0.02 grams of potassium dihydrogen phosphate, 0.05 grams of magnesium sulfate, 0.03 grams of calcium chloride, sulfurous acid 0.01 grams of iron, 0.01 grams of NA-EDTA, 1 ml of trace element mother liquor, each liter of trace element mother liquor contains: 0.01 grams of copper sulfate pentahydrate, 0.01 grams of zinc sulfate heptahydrate, 0.01 grams of cobalt chloride hexahydrate, 0.1 gram of manganese chloride tetrahydrate, 0.01 gram of sodium molybdate dihydrate, 1 ml of vitamin mother solution, and each liter of vitamin mother solution contains: 0.1 gram of vitamin B1, 0.1 gram of vitamin B6, 0.1 gram of vitamin B12, and 0.1 gram of biotin , the pH value of the medium is 6, and then use a UV lamp to sterilize f...

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Abstract

The invention discloses a high-density continuous culture method of nannochloropsis. The method comprises the following steps: preparing a culture medium in a closed photoreactor, performing sterilization by using an ultraviolet lamp for 30 min, and inoculating the nannochloropsis grown to an exponential growth phase into the above culture medium, wherein the inoculation amount of the nannochloropsis culture solution is 5-20%; and performing culture by using an artificial LED light source, wherein the culture temperature is 20-30 DEG C, performing irradiation by using monochromatic white lightfor 3-5 days, wherein the irradiation intensity of the white light is 500-2000 lx, when the culture is performed to a fifth day, releasing half of the culture solution, adding half of a fresh culturemedium through flowing, continuing fermentation, and afterward releasing half of volume every 2 days as a fermentation liquid of the nannochloropsis, wherein 10 batches are continuously added. The high-density continuous culture method of the nannochloropsis can continuously provide a light source for the culture of the nannochloropsis, so that the nannochloropsis can be subjected to high-densityculture, the culture work of the nannochloropsis can be continuously carried out, and the continuous culture of the nannochloropsis can be completed.

Description

technical field [0001] The invention relates to the technical field of Nannochloropsis culture, in particular to a high-density continuous culture method of Nannochloropsis. Background technique [0002] Microalgae, that is, microalgae, range in size from a few microns to hundreds of microns, and can efficiently produce organic substances such as lipids, proteins, and polysaccharides. Among them, lipids can be converted into biodiesel through transesterification, while Nannochloropsis The lipid ratio is as high as 68%. As a single-celled algae, Nannochloropsis not only has a high lipid content, but also has the advantages of strong environmental adaptability, small individuals, and fast reproduction. It ranks among the excellent algal species for biodiesel production . [0003] At present, the demand for Nannochloropsis is relatively large, but there are certain problems in some cultivation methods of Nannochloropsis. Nannochloropsis can be obtained after culturing for a f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 郭立赵阳娟何加发梁薏琳邵华
Owner ZHANJIANG GUOLIAN AQUATIC PROD CO LTD
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