A method for extracting epa in Nannochloropsis by compound enzyme method

A technology of Nannochloropsis and compound enzymes, which is applied in the separation/purification of carboxylic acid compounds, bulk chemical production, organic chemistry, etc., and can solve the problem of unsatisfactory extraction purity, limited extraction conditions, and further improvement of process conditions and other problems, to achieve the effect of less pollution, easy and safe operation, and increased fluidity

Active Publication Date: 2021-05-28
QINGDAO LANGYATAI GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is reported in the prior art to extract by aqueous enzymatic method, yet its extraction purity is not ideal, that is to say, the process extraction conditions in the prior art are limited, and the process conditions need to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The first step is to centrifuge the Nannochloropsis fermented liquid at a speed of 3600rpm for 20 minutes to obtain the supernatant and algae mud, and collect the algae mud; select activated carbon in the suction filter barrel: diatomaceous earth = 1:1 to build a filter cake , after the supernatant is suction-filtered and adsorbed, it is used to configure a new medium, which can be recycled to achieve clean production. 5.0 kg can process 200 liters of supernatant;

[0025] The second step, adding pH to the algae mud is a phosphate buffer solution with a pH of 7.5, the ratio of material to water is 1:6 (g / mL), the amount of chitosanase added is 0.8% of the weight of the algae mud, and the amount of fructose enzyme added is 0.8% of the weight of the algae mud. 0.5% by weight, the amount of alkaline protease added is 1.8% by weight of algae mud, hydrolyzed for 0.8h at an enzymolysis temperature of 40°C to obtain an enzymolysis solution;

[0026] The third step is to centri...

Embodiment 2

[0030] The first step is to centrifuge the Nannochloropsis fermented liquid at a speed of 3600rpm for 20 minutes to obtain the supernatant and algae mud, and collect the algae mud; select activated carbon in the suction filter barrel: diatomaceous earth = 1:1 to build a filter cake , after the supernatant is suction-filtered and adsorbed, it is used to configure a new medium, which can be recycled to achieve clean production. 5.0 kg can process 200 liters of supernatant;

[0031] The second step is to add a phosphate buffer solution with a pH of 7.5 to the algae mud, the ratio of material to water is 1:6 (g / mL), the compound enzyme is chitosanase, fructose and alkaline protease, and the amount of chitosanase added 0.7% of the weight of the algae mud, the amount of fructose added is 0.6% of the weight of the algae mud, the amount of alkaline protease added is 1.9% of the weight of the algae mud, and the hydrolysis temperature is 40°C for 0.8 hours to obtain an enzymolyzed soluti...

Embodiment 3

[0036] The first step is to centrifuge the Nannochloropsis fermented liquid at a speed of 3600rpm for 20 minutes to obtain the supernatant and algae mud, and collect the algae mud; use activated carbon in the suction filter bucket: diatomaceous earth = 1:1 to build a filter cake , after the supernatant is suction-filtered and adsorbed, it is used to configure a new medium, which can be recycled to achieve clean production. 5.0 kg can process 200 liters of supernatant;

[0037] The second step is to add a phosphate buffer solution with a pH of 7.5 to the algae mud, the ratio of material to water is 1:6 (g / mL), the compound enzyme is hemicellulase, alkaline protease and trypsin, and the addition of hemicellulase The amount is 0.7% of the weight of the algae mud, the amount of alkaline protease added is 0.6% of the weight of the algae mud, the amount of trypsin added is 1.9% of the weight of the algae mud, and the hydrolysis temperature is 50°C for 0.6h to obtain the enzymolyzed s...

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Abstract

The invention discloses a method for extracting EPA in Nannochloropsis algae by a compound enzyme method. The method comprises: centrifuging the fermented liquid of Nannochloropsis algae to collect the algae mud; adding a certain amount of buffer solution to the algae mud, and then adding Composite enzymes, react to obtain enzymolysis solution; centrifuge the enzymolysis solution to obtain the first free oil and emulsion, further demulsify the emulsion and centrifuge to obtain the second free oil, combine the first free oil and the second free oil to obtain Crude oil; the resulting crude oil is subjected to supercritical CO 2 Purification method Further purification to obtain EPA. The present invention treats Nannochloropsis with complex enzymes, and under certain reaction conditions, enzymatically destroys the microalgae cell structure, and at the same time strengthens the degradation of complexes such as lipopolysaccharide and lipoprotein, increases the fluidity of oil, and frees oil . The extraction purity of the EPA of the present invention is above 95%.

Description

technical field [0001] The invention relates to the technical field of biological extraction, in particular to a method for extracting EPA in Nannochloropsis by a compound enzyme method. Background technique [0002] EPA is Eicosapentaenoic acid (Eicosapentaenoic Acid), which has various physiological functions such as promoting cell metabolism, lowering cholesterol, relieving arthritis, preventing and treating cardiovascular and cerebrovascular diseases, and is widely used in human health care and emerging pharmaceutical industries. Natural EPA was mainly obtained from fish oil in the past, but the composition of fish oil is relatively complex, so it is difficult to extract high-purity EPA, which makes it difficult to exert its efficacy; and due to the reduction of fish resources worldwide, it is extracted from fish oil EPA has been unable to meet the increasing market demand. [0003] Nannochloropsis sp. is a marine unicellular microalgae with small algal cells with a dia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/42C07C51/48C07C57/03C11B1/02C11B1/04C12P7/64
CPCC07C51/42C07C51/48C11B1/025C11B1/04C12P7/6427C07C57/03Y02P20/54
Inventor 李悦明许丽娜徐建春夏修峦徐炳政
Owner QINGDAO LANGYATAI GRP
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