Anoectochilus roxburghii extracting solution and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine extraction, and discloses an anoectochilus formosanus extracting solution and a preparation method thereof. The preparation method comprises the following steps: S1, pretreatment; s2, obtaining anoectochilus roxburghii particles; s3, extracting active pharmaceutical ingredients in the anoectochilus formosanus granules by taking supercritical CO2 as a solvent and adding an entrainer; s4, performing auxiliary extraction by means of ultrasonic treatment while performing S3; s5, the pressure of the supercritical CO2 fluid is reduced, and active components are separated out; s6, performing concentration and purification treatment to obtain an extract; and S7, adding an antioxidant, honey and a flocculant into the extract, and mixing to obtain the anoectochilus formosanus extracting solution. The whole preparation process is good in resource utilization, the quality and efficiency of the herbaceous plant anoectochilus formosanus can be efficiently improved, the relative concentration difference is always kept between the anoectochilus formosanus and the solvent, and the extraction rate of medicine components is increased.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a roxburghii roxburghii extract and a method for preparing the extract. Background Art

[0002] According to the "Chinese Materia Medica" and "Fujian Pharmacopoeia", the whole plant of Anoectochilus roxburghii can be used as medicine. It is mild, cool, sweet, and enters the lung, liver, kidney, and bladder meridians. It has the effects of clearing heat and cooling blood, removing dampness and detoxifying, calming the liver and promoting diuresis, and anti-inflammatory. Anoectochilus roxburghii extract is widely used in many fields. For example, Anoectochilus roxburghii extract performs well in the prevention and treatment of acute liver injury, and can significantly improve the symptoms of acute liver injury induced by D-galactosamine. Anoectochilus roxburghii extract also has anti-cancer effects and can be used in the preparation of anti-cancer drugs. Anoectochilus roxburghii extract can inhibit the production of melanin and is suitable for natural whitening skin care products.

[0003] The traditional method of extracting golden thread vine requires a long period of water decoction or soaking, resulting in a long production cycle and low efficiency. In addition, as the water decoction continues, the solvent evaporates and the concentration decreases, which affects the extraction effect of the medicinal components in the golden thread vine and reduces the extraction rate of the medicinal components. Summary of the invention

[0004] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a roxburghii extract and a preparation method thereof to efficiently improve the quality and efficiency of the herb roxburghii, and the entire preparation process is pollution-free and has good resource utilization.

[0005] A method for preparing a roxburghii extract comprises the following steps:

[0006] S1 takes Anoectochilus roxburghii for pretreatment;

[0007] S2 dry-mills the pretreated roxburghii to obtain roxburghii particles;

[0008] S3 uses supercritical CO2 as solvent and adds entraining agent to extract the active pharmaceutical ingredients from Anoectochilus roxburghii granules;

[0009] S4 performs auxiliary extraction with the aid of ultrasonic treatment while performing S3;

[0010] S5 reduces the pressure of supercritical CO2 fluid, and the active components are separated out;

[0011] S6 is concentrated and purified to obtain an extract;

[0012] S7: adding antioxidant, honey and flocculant to the extract, mixing to obtain a roxburghii extract.

[0013] As a further improvement of the above scheme, the method for pre-treating the roxburghii is specifically performed as follows: picking the roxburghii plants with complete phases, washing them with water, placing them in a heat pump dryer, and drying them at 50-80° C. for 4-8 hours. In the present invention, the polysaccharide yield of the roxburghii dehydrated by the heat pump is higher, and the required drying time is shorter.

[0014] As a further improvement of the above scheme, the ball milling method is specifically operated as follows: put the dried golden thread vine into a ball mill at a speed of 200 to 300 r / min and ball mill for 30 to 45 minutes. The present invention obtains micron-sized golden thread vine particles through ball milling, thereby increasing the contact area with the extraction solvent during subsequent extraction, improving the extraction efficiency and the acquisition rate of active pharmaceutical ingredients.

[0015] As a further improvement of the above scheme, the active pharmaceutical ingredients include anoectin, alkaloids, flavonoids, steroids and triterpenoids. The flavonoids are quercetin and isorhamnetin, the steroids are lanasterol, ergosterol, stigmasterol, campesterol and β-sitosterol, and the triterpenoids are suberenone, oleanolic acid and ursolic acid.

[0016] As a further improvement of the above scheme, the method of extracting the active pharmaceutical ingredients in the golden thread lotus particles using supercritical CO2 as a solvent is specifically performed as follows:

[0017] S31: dispersing the roxburghii granules in pure water, adding an entraining agent, mixing, and putting into a supercritical extraction kettle;

[0018] S32 controls the temperature of the supercritical extraction kettle to 30-60°C, and introduces supercritical CO2 fluid; the flow rate of the supercritical CO2 is 0.4-0.6 ml / min, and the extraction time is 2-5 hours. When the temperature of carbon dioxide is higher than the critical temperature of 31.26°C and the pressure is higher than the critical pressure of 72.9 atm, the properties of carbon dioxide will change. Its density is close to that of liquid, its viscosity is close to that of gas, and its diffusion coefficient is 100 times that of liquid. It can dissolve a variety of substances. In the present invention, supercritical CO2 has high solubility and diffusivity, and can quickly and efficiently extract the medicinal ingredients in golden thread lotus.

[0019] As a further improvement of the above scheme, the ultrasonic treatment time is 20 to 30 minutes, and the ultrasonic frequency is 20 to 40 kHz. The present invention utilizes the cavitation effect generated by ultrasound to enable the solvent to fully and quickly penetrate into the interior of the golden thread lotus particles, thereby increasing the solubility of the active drug ingredients in the solvent and having a heating effect, which is beneficial to the leaching of the active drug ingredients.

[0020] As a further improvement of the above scheme, the active ingredient solution is placed in a rotary evaporator and concentrated to 5% to 15% of the original volume, and then purified using 732 cation exchange resin.

[0021] A roxburghii extract comprises the following components: roxburghii extract, antioxidant, honey and flocculant are mixed in a ratio of 8-25:1-3:2-4:1 to obtain the extract.

[0022] As a further improvement of the above scheme, the weight proportion of the extract is as follows: 80-120 parts of Anoectochilus roxburghii extract, 7-12 parts of antioxidant, 15-25 parts of honey, and 6-10 parts of flocculant. In the present invention, the extract of this embodiment, wherein the Anoectochilus roxburghii extract has a variety of active pharmaceutical ingredients, can enhance immunity, the added antioxidant plays a role in preventing the effective ingredients in the liquid from being oxidized and prolonging the shelf life, the flocculant is added to the preparation, so that the product can be restored to a uniformly dispersed suspension after shaking, avoiding sedimentation to facilitate taking, and the honey plays a role in sweetening, so as to facilitate the taking of the extract.

[0023] As a further improvement of the above scheme, the antioxidant is any one of sodium sulfite, sodium bisulfite, sodium pyrosulfite and vitamin C. The flocculant is any one of citrate, hydrogen citrate, tartrate, hydrogen tartrate and phosphate.

[0024] As a further improvement of the above scheme, the extract is prepared by the preparation method of the roxburghii roxburghii extract.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] The present invention uses supercritical CO2 as a solvent, and the active drug components can be precipitated by subsequently changing the pressure to achieve separation. Moreover, the supercritical CO2 fluid can be reused after separation. The entire preparation process has good resource utilization and can efficiently improve the quality and efficiency of the herb Anoectochilus roxburghii.

[0027] The present invention continuously introduces supercritical CO2 during extraction, so that the relative concentration difference between the roxburghii and the solvent is always maintained, thereby improving the dissolution efficiency of the drug components in the roxburghii into the supercritical CO2 and improving the extraction rate of the drug components. DETAILED DESCRIPTION

[0028] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with the embodiments. Additional aspects and advantages of the present invention will be given in part in the following description, and will become apparent from the following description, or will be understood through the practice of the present invention. It should be understood that the following description is only used to explain the present invention and is not intended to limit the present invention.

[0029] As used herein, the terms "comprises," "including," "contains," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0030] When an amount, concentration, or other value or parameter is expressed as a range, a preferred range, or a range limited by a series of upper preferred values ​​and lower preferred values, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted as including a range of "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5" etc. When a numerical range is described in this article, unless otherwise specified, the range is intended to include its end values ​​and all integers and fractions within the range.

[0031] The specific embodiments of the present invention are described in detail below.

[0032] Example 1

[0033] The present embodiment provides a roxburghii extract, which is composed of 80 parts of roxburghii extract, 7 parts of sodium sulfite, 15 parts of honey, and 6 parts of citrate. The extract of the present embodiment, wherein the roxburghii extract has a variety of active pharmaceutical ingredients, can enhance immunity, the added sodium sulfite prevents the effective ingredients in the liquid from being oxidized and prolongs the shelf life, the addition of citrate in the preparation allows the product to be restored to a uniformly dispersed suspension after shaking, avoiding sedimentation to facilitate taking, and the honey plays a role in sweetening, so that the extract can be taken.

[0034] The method for preparing the extract of Anoectochilus roxburghii in this embodiment comprises the following steps:

[0035] S1: picking complete roxburghii plants, washing them with water, placing them in a heat pump dryer, and drying them at 50° C. for 8 hours. In this embodiment, the polysaccharide yield of roxburghii dehydrated by heat pump is higher, and the required drying time is shorter.

[0036] S2: putting the dried Anoectochilus roxburghii into a ball mill at a speed of 200 r / min for 45 min to obtain Anoectochilus roxburghii particles. In this embodiment, the Anoectochilus roxburghii particles are obtained by ball milling to increase the contact area with the extraction solvent during subsequent extraction, thereby improving the extraction efficiency and the acquisition rate of the active pharmaceutical ingredient.

[0037] S3 uses supercritical CO2 as solvent and adds entraining agent to extract active pharmaceutical ingredients in the golden thread lotus particles, and the active pharmaceutical ingredients include golden thread glycosides, alkaloids, flavonoids, steroids and triterpenoids. The flavonoids are quercetin and isorhamnetin, and the steroids are lansterol, ergosterol, stigmasterol, campesterol and β-sitosterol. The triterpenoids are suberinone, oleanolic acid and ursolic acid. The entraining agent uses methanol, ethanol, acetone or ethylamine. In this embodiment, the entraining agent is added to promote the extraction of strong polar substances such as golden thread glycosides, quercetin, isorhamnetin and some alkaloids in the golden thread lotus.

[0038] The method of extracting the active pharmaceutical ingredients in the golden thread lotus granules by using supercritical CO2 as solvent and adding a carrier is as follows:

[0039] S31: Disperse the roxburghii granules in pure water in a weight ratio of pure water to the roxburghii granules of 1:1, and put them into a supercritical extraction kettle.

[0040] S32 controls the temperature of the supercritical extraction kettle to 30°C, introduces supercritical CO2 fluid, the flow rate of supercritical CO2 is 0.4ml / min, and the extraction time is 2h. When the temperature of carbon dioxide is higher than the critical temperature of 31.26°C and the pressure is higher than the critical pressure of 72.9atm, the properties of carbon dioxide will change. Its density is close to that of liquid, its viscosity is close to that of gas, and its diffusion coefficient is 100 times that of liquid. It can dissolve a variety of substances. In this embodiment, supercritical CO2 has high solubility and diffusivity, and can quickly and efficiently extract the medicinal ingredients in golden thread lotus.

[0041] S4 performs auxiliary extraction with the aid of ultrasonic treatment while performing S3, the ultrasonic treatment time is 20 min, and the ultrasonic frequency is 20 kHz. In this embodiment, the cavitation effect generated by the ultrasonic wave can make the solvent fully and quickly penetrate into the inside of the golden thread lotus particles, improve the solubility of the active pharmaceutical ingredient in the solvent, and have a heating effect, which is conducive to the leaching of the active pharmaceutical ingredient.

[0042] S5 reduces the pressure of supercritical CO2 fluid, and the active components are separated out;

[0043] S6: Concentration and purification to obtain an extract. The active ingredient solution is placed in a rotary evaporator and concentrated to 5% of the original volume, and then purified using 732 cation exchange resin to remove impurities. The active ingredient in the obtained extract has a high purity.

[0044] S7: adding sodium sulfite, honey and citrate to the extract, and mixing to obtain a roxburghii extract.

[0045] Example 2

[0046] The present embodiment provides a roxburghii extract, which is composed of the following by weight: 90 parts of roxburghii extract, 9 parts of sodium bisulfite, 18 parts of honey, and 7 parts of hydrogen citrate. In the extract of the present embodiment, the roxburghii extract has multiple active pharmaceutical ingredients and can enhance immunity. The added sodium bisulfite prevents the effective ingredients in the liquid from being oxidized and prolongs the shelf life. The addition of hydrogen citrate in the preparation allows the product to be restored to a uniformly dispersed suspension after shaking, avoiding sedimentation to facilitate taking, and the honey sweetens the extract to facilitate taking.

[0047] The method for preparing the extract of Anoectochilus roxburghii in this embodiment comprises the following steps:

[0048] S1: picking complete roxburghii plants, washing them with water, placing them in a heat pump dryer, and drying them at 60° C. for 7 hours. In this embodiment, the polysaccharide yield of roxburghii dehydrated by heat pump is higher, and the required drying time is shorter.

[0049] S2: taking the dried Anoectochilus roxburghii and putting it into a ball mill at a speed of 240 r / min for 35 min to obtain Anoectochilus roxburghii particles. In this embodiment, the Anoectochilus roxburghii particles of micron size are obtained by ball milling, thereby increasing the contact area with the extraction solvent during subsequent extraction, improving the extraction efficiency and the acquisition rate of the active pharmaceutical ingredient.

[0050] S3 uses supercritical CO2 as solvent and adds ethanol to extract the active pharmaceutical ingredients in the golden thread lotus particles, and the active pharmaceutical ingredients include golden thread glycosides, alkaloids, flavonoids, steroids and triterpenoids. The flavonoids are quercetin and isorhamnetin, and the steroids are lansterol, ergosterol, stigmasterol, campesterol and beta-sitosterol. The triterpenoids are suberinone, oleanolic acid and ursolic acid. In the present embodiment, ethanol is added to promote the extraction of strong polar substances such as golden thread glycosides, quercetin, isorhamnetin and some alkaloids in the golden thread lotus.

[0051] The method of extracting the active pharmaceutical ingredients in the golden thread lotus granules using supercritical CO2 as a solvent is as follows:

[0052] S31: Disperse the roxburghii granules in pure water, with the weight ratio of pure water to the roxburghii granules being 1.5:1, add ethanol, mix, and put into a supercritical extraction kettle.

[0053] S32 controls the temperature of the supercritical extraction kettle to 40°C, introduces supercritical CO2 fluid, the flow rate of supercritical CO2 is 0.5ml / min, and the extraction time is 3h. When the temperature of carbon dioxide is higher than the critical temperature of 31.26°C and the pressure is higher than the critical pressure of 72.9atm, the properties of carbon dioxide will change. Its density is close to that of liquid, its viscosity is close to that of gas, and its diffusion coefficient is 100 times that of liquid. It can dissolve a variety of substances. In this embodiment, supercritical CO2 has high solubility and diffusivity, and can quickly and efficiently extract the medicinal ingredients in golden thread lotus.

[0054] S4 performs auxiliary extraction with the aid of ultrasonic treatment while performing S3, the ultrasonic treatment time is 23 minutes, and the ultrasonic frequency is 30kHz. In this embodiment, the cavitation effect generated by the ultrasonic wave can make the solvent fully and quickly penetrate into the inside of the golden thread lotus particles, improve the solubility of the active pharmaceutical ingredient in the solvent, and have a heating effect, which is conducive to the leaching of the active pharmaceutical ingredient.

[0055] S5 reduces the pressure of supercritical CO2 fluid, and the active components are separated out;

[0056] S6: Concentration and purification to obtain an extract. The active ingredient solution is placed in a rotary evaporator and concentrated to 10% of the original volume, and then purified using 732 cation exchange resin to remove impurities. The active ingredient in the obtained extract has a high purity.

[0057] S7: adding sodium bisulfite, honey and hydrogen citrate to the extract, and mixing to obtain a roxburghii extract.

[0058] Example 3

[0059] The present embodiment provides a roxburghii extract, which is composed of the following parts by weight: 110 parts of roxburghii extract, 11 parts of sodium pyrosulfite, 24 parts of honey, and 9 parts of tartrate. The extract of the present embodiment, wherein the roxburghii extract has a variety of active pharmaceutical ingredients, can enhance immunity, the added sodium pyrosulfite prevents the effective ingredients in the liquid from being oxidized and prolongs the shelf life, the addition of tartrate in the preparation allows the product to be restored to a uniformly dispersed suspension after shaking, avoiding sedimentation to facilitate taking, and the honey plays a role in sweetening, so as to facilitate the taking of the extract.

[0060] The method for preparing the extract of Anoectochilus roxburghii in this embodiment comprises the following steps:

[0061] S1: picking complete roxburghii plants, washing them with water, placing them in a heat pump dryer, and drying them at 70° C. for 6 hours. In this embodiment, the polysaccharide yield of roxburghii dehydrated by heat pump is higher, and the required drying time is shorter.

[0062] S2: taking the dried Anoectochilus roxburghii and putting it into a ball mill at a speed of 280 r / min for 40 min to obtain Anoectochilus roxburghii particles. In this embodiment, the Anoectochilus roxburghii particles of micron size are obtained by ball milling, thereby increasing the contact area with the extraction solvent during subsequent extraction, improving the extraction efficiency and the acquisition rate of the active pharmaceutical ingredient.

[0063] S3 uses supercritical CO2 as solvent and adds ethanol to extract the active pharmaceutical ingredients in the golden thread lotus particles, and the active pharmaceutical ingredients include golden thread glycosides, alkaloids, flavonoids, steroids and triterpenoids. The flavonoids are quercetin and isorhamnetin, and the steroids are lansterol, ergosterol, stigmasterol, campesterol and beta-sitosterol. The triterpenoids are suberinone, oleanolic acid and ursolic acid. In the present embodiment, ethanol is added to promote the extraction of strong polar substances such as golden thread glycosides, quercetin, isorhamnetin and some alkaloids in the golden thread lotus.

[0064] The method of extracting the active pharmaceutical ingredients in the golden thread lotus granules using supercritical CO2 as a solvent is as follows:

[0065] S31: Disperse the roxburghii granules in pure water, wherein the weight ratio of pure water to the roxburghii granules is 1.8:1, add ethanol, mix, and put into a supercritical extraction kettle.

[0066] S32 controls the temperature of the supercritical extraction kettle to 50°C, introduces supercritical CO2 fluid, the flow rate of supercritical CO2 is 0.55ml / min, and the extraction time is 4h. When the temperature of carbon dioxide is higher than the critical temperature of 31.26°C and the pressure is higher than the critical pressure of 72.9atm, the properties of carbon dioxide will change. Its density is close to that of liquid, its viscosity is close to that of gas, and its diffusion coefficient is 100 times that of liquid. It can dissolve a variety of substances. In this embodiment, supercritical CO2 has high solubility and diffusivity, and can quickly and efficiently extract the medicinal ingredients in golden thread lotus.

[0067] S4 performs auxiliary extraction with the aid of ultrasonic treatment while performing S3, the ultrasonic treatment time is 27 minutes, and the ultrasonic frequency is 35kHz. In this embodiment, the cavitation effect generated by ultrasonic waves can make the solvent fully and quickly penetrate into the inside of the golden thread lotus particles, improve the solubility of the active pharmaceutical ingredient in the solvent, and have a heating effect, which is conducive to the leaching of the active pharmaceutical ingredient.

[0068] S5 reduces the pressure of supercritical CO2 fluid, and the active components are separated out;

[0069] S6 Concentration and purification treatment to obtain an extract. The active ingredient solution is placed in a rotary evaporator and concentrated to 12% of the original volume, and then purified using 732 cation exchange resin to remove impurities. The purity of the active ingredient in the obtained extract is relatively high.

[0070] S7: adding sodium pyrosulfite, honey and tartrate to the extract, and mixing to obtain a roxburghii extract.

[0071] Example 4

[0072] The present embodiment provides a roxburghii extract, which is composed of the following parts by weight: 120 parts of roxburghii extract, 12 parts of vitamin C, 25 parts of honey, and 10 parts of phosphate. The extract of the present embodiment, wherein the roxburghii extract has a variety of active pharmaceutical ingredients, can enhance immunity, the added vitamin C prevents the effective ingredients in the liquid from being oxidized and prolongs the shelf life, the addition of phosphate in the preparation allows the product to be restored to a uniformly dispersed suspension after shaking, avoiding sedimentation to facilitate taking, and the honey plays a role in sweetening, so that the extract can be taken.

[0073] The method for preparing the extract of Anoectochilus roxburghii in this embodiment comprises the following steps:

[0074] S1: picking complete roxburghii plants, washing them with water, placing them in a heat pump dryer, and drying them at 80° C. for 5 hours. In this embodiment, the polysaccharide yield of roxburghii dehydrated by heat pump is higher, and the required drying time is shorter.

[0075] S2: putting the dried Anoectochilus roxburghii into a ball mill at a speed of 300 r / min for 30 min to obtain Anoectochilus roxburghii particles. In this embodiment, the Anoectochilus roxburghii particles are obtained by ball milling to increase the contact area with the extraction solvent during subsequent extraction, thereby improving the extraction efficiency and the acquisition rate of the active pharmaceutical ingredient.

[0076] S3 uses supercritical CO2 as solvent and adds ethanol to extract the active pharmaceutical ingredients in the golden thread lotus particles, and the active pharmaceutical ingredients include golden thread glycosides, alkaloids, flavonoids, steroids and triterpenoids. The flavonoids are quercetin and isorhamnetin, and the steroids are lansterol, ergosterol, stigmasterol, campesterol and beta-sitosterol. The triterpenoids are suberinone, oleanolic acid and ursolic acid. In the present embodiment, ethanol is added to promote the extraction of strong polar substances such as golden thread glycosides, quercetin, isorhamnetin and some alkaloids in the golden thread lotus.

[0077] The method of extracting the active pharmaceutical ingredients in the golden thread lotus granules using supercritical CO2 as a solvent is as follows:

[0078] S31: Disperse the roxburghii granules in pure water, wherein the weight ratio of pure water to the roxburghii granules is 2:1, add ethanol, mix, and put into a supercritical extraction kettle.

[0079] S32 controls the temperature of the supercritical extraction kettle to 60°C, introduces supercritical CO2 fluid, the flow rate of supercritical CO2 is 0.6ml / min, and the extraction time is 5h. When the temperature of carbon dioxide is higher than the critical temperature of 31.26°C and the pressure is higher than the critical pressure of 72.9atm, the properties of carbon dioxide will change. Its density is close to that of liquid, its viscosity is close to that of gas, and its diffusion coefficient is 100 times that of liquid. It can dissolve a variety of substances. In this embodiment, supercritical CO2 has high solubility and diffusivity, and can quickly and efficiently extract the medicinal ingredients in golden thread lotus.

[0080] S4 performs auxiliary extraction with the aid of ultrasonic treatment while performing S3, the ultrasonic treatment time is 30 minutes, and the ultrasonic frequency is 40kHz. In this embodiment, the cavitation effect generated by the ultrasonic wave can make the solvent fully and quickly penetrate into the inside of the golden thread lotus particles, improve the solubility of the active pharmaceutical ingredient in the solvent, and have a heating effect, which is conducive to the leaching of the active pharmaceutical ingredient.

[0081] S5 reduces the pressure of supercritical CO2 fluid, and the active components are separated out;

[0082] S6 Concentration and purification treatment to obtain an extract. The active ingredient solution is placed in a rotary evaporator and concentrated to 15% of the original volume, and then purified using 732 cation exchange resin to remove impurities. The active ingredient in the obtained extract has a high purity.

[0083] S7 adds vitamin C, honey and phosphate to the extract, and mixes them to obtain a roxburghii extract.

[0084] The above embodiments are merely preferred implementations of the present invention. Any simple modification, amendment and substitution of the above embodiments based on the technical essence of the present invention shall fall within the scope of the technical solution of the present invention.

Claims

1. A method for preparing a roxburghii extract, characterized in that: The following steps are involved: S1 takes Anoectochilus roxburghii for pretreatment; S2 dry-mills the pretreated roxburghii to obtain roxburghii particles; S3 uses supercritical CO2 as solvent and adds entraining agent to extract the active pharmaceutical ingredients from Anoectochilus roxburghii granules; S4 performs auxiliary extraction with the aid of ultrasonic treatment while performing S3; S5 reduces the pressure of supercritical CO2 fluid, and the active components are separated out; S6 is concentrated and purified to obtain an extract; S7: adding antioxidant, honey and flocculant to the extract, mixing to obtain a roxburghii extract.

2. The method for preparing the roxburghii extract according to claim 1, characterized in that: The method for pre-treating the roxburghii roxburghii is specifically performed as follows: picking intact roxburghii roxburghii plants, washing them with water, placing them in a heat pump dryer, and drying them at 50-80° C. for 4-8 hours.

3. The method for preparing the roxburghii extract according to claim 2, characterized in that: The ball milling method is specifically operated as follows: put the dried golden thread vine into a ball mill at a rotation speed of 200 to 300 r / min and perform ball milling for 30 to 45 minutes.

4. The method for preparing the roxburghii extract according to claim 1, characterized in that: The active pharmaceutical ingredients include anoectochilus glycosides, alkaloids, flavonoids, steroids and triterpenes; The flavonoid compounds are quercetin and isorhamnetin, the steroid compounds are lanasterol, ergosterol, stigmasterol, campesterol and beta-sitosterol; and the triterpenoid compounds are suberinone, oleanolic acid and ursolic acid.

5. The method for preparing the roxburghii extract according to claim 4, characterized in that: The method of using supercritical CO2 as a solvent and adding a carrier to extract the active pharmaceutical ingredient in the golden thread lotus particles is specifically performed as follows: S31: dispersing the roxburghii granules in pure water, adding an entraining agent, mixing, and putting into a supercritical extraction kettle; S32 controls the temperature of the supercritical extraction kettle to 30-60°C and introduces supercritical CO2 fluid; The flow rate of the supercritical CO2 is 0.4-0.6 ml / min, and the extraction time is 2-5 hours.

6. The method for preparing the roxburghii roxburghii extract according to claim 1, characterized in that: The ultrasonic treatment time is 20 to 30 minutes, and the ultrasonic frequency is 20 to 40 kHz.

7. The method for preparing the roxburghii extract according to claim 1, characterized in that: The active ingredient solution was placed in a rotary evaporator and concentrated to 5% to 15% of the original volume, and then purified using 732 cation exchange resin.

8. A roxburghii extract, characterized in that: The extract has the following components: anoectochilus roxburghii extract, an antioxidant, honey and a flocculant are mixed in a ratio of 8-25:1-3:2-4:1 to obtain the extract.

9. The extract of Anoectochilus roxburghii according to claim 8, characterized in that The extract is composed of the following parts by weight: 80-120 parts of roxburghii extract, 7-12 parts of antioxidant, 15-25 parts of honey, and 6-10 parts of flocculant; The antioxidant is any one of sodium sulfite, sodium bisulfite, sodium pyrosulfite and vitamin C; the flocculant is any one of citrate, hydrogen citrate, tartrate, hydrogen tartrate and phosphate.

10. The extract of Anoectochilus roxburghii according to claim 9, characterized in that The extract is prepared by the preparation method of the roxburghii roxburghii extract according to any one of claims 1 to 7.