Bacillus pumilus shou002 and application thereof

A technology of Bacillus pumilus and aquatic pathogens, applied in the field of microorganisms, can solve problems such as drug residues, environmental hazards, high morbidity and mortality

Inactive Publication Date: 2014-07-23
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in recent years, with the vigorous development of large yellow croaker cage culture, various fish diseases have also occurred frequently, especially vibriosis, which has a long onset time and high morbidity and mortality, causing serious economic losses to fishery production.
Long-term use of traditional antibiotics and other drugs will cause adverse consequences such as multi-drug resistance of bacteria, drug residues, and environmental damage

Method used

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  • Bacillus pumilus shou002 and application thereof
  • Bacillus pumilus shou002 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The screening of embodiment 1 bacillus pumilus shou002

[0018] Using manganese nutrient agar, thiosulfate citrate bile salt sucrose agar (TCBS), eosin methylene blue agar (EMB), seawater agar (2216E) and freshwater agar (FWA) medium to separate and screen from large yellow croaker intestine Antagonistic bacteria with strong antagonistic effect on Vibrio parahaemolyticus (Vp1.2164) and Vibrio alginolyticus (Va-Y) were produced.

[0019] In vitro antagonistic experiments were carried out on the screened strains by the spot method, and the strains with the broadest antibacterial spectrum and the strongest effect were screened. The strains were identified by bacteria identification instrument and 16Sr RNA gene sequence analysis to determine their taxonomic status.

[0020] Select a strain with strong antagonistic effect and wide antibacterial spectrum, and through the VITEK-32 rapid bacterial identification instrument and 16Sr RNA gene sequence analysis, it is determined ...

Embodiment 2

[0021] Example 2 Bacillus pumilus shou002 extracellular product inhibits pathogenic Vibrio

[0022] (1) Referring to Sugita et al. (Sugita H, Hirose Y, Matsue N, et al. Vibrio sp.strain NM10, isolated from the intestine of a Japanese coastal fish, has an inhibitory effect against Pasteurella piscicida[J].Appli Environ Microbiol, 1998, 64: 231-237), the specific steps are as follows: activate the screened antagonistic bacteria on the slant of nutrient agar and inoculate them in nutrient broth medium, culture at 28°C for a certain period of time with constant temperature shaking, 4°C, 9600rpm / Centrifuge under the condition of 20 min for 20 min, discard the cell pellet, and filter the supernatant through a 0.22 μm microporous membrane, and the obtained liquid is the extracellular product of Bacillus pumilus shou002, which is stored at 4 °C for later use.

[0023] (2) Antibacterial activity was detected by plate diffusion method.

[0024] Vibrio parahaemolyticus (Vibrio parahaem...

Embodiment 3

[0026] The safety of embodiment 3 bacillus pumilus shou002

[0027] The nutrient broth culture medium (including 10g of peptone, 3g of beef extract powder, 5g of sodium chloride, 1000ml of distilled water, heated and dissolved, divided into flasks, and sterilized at 121°C for 15 minutes) was streaked on sheep blood. On the plate, cultured at 28°C for 24 hours, the production of hemolysin was judged according to the formation of a transparent circle around the colony, and Vibrio Vp1.2164 was used as a positive control. The test results showed that Vibrio parahaemolyticus 1.2164 had hemolysis on the blood plate, but Bacillus pumilus shou002 had no hemolysis on the blood plate, indicating that the strain did not produce hemolysin and had no potential pathogenicity.

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Abstract

The present invention provides Bacillus pumilus shou002 and an application thereof, wherein the Bacillus pumilus shou002 is preserved in the China Center for Type Culture Collection on November 3, 2013, has the preservation number of CCTCC NO:M2013536, provides good inhibition effects for aquatic pathogenic bacteria, has characteristics of no pathogenicity, safety, reliability, effective degradation of organic matters, ammonia nitrogen, nitrite nitrogen and other pollutants in breeding water bodies, breeding environment optimizing, and promotion of water environment ecology beneficial cycle, and can be used as the feed additive and the water body ecology environment modifier so as to be used in aquaculture.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus pumilus shou002 and an application thereof. Background technique [0002] Large yellow croaker (Pseudosciaena crocea) is one of the main marine economic fish in my country. However, in recent years, with the vigorous development of large yellow croaker cage culture, various fish diseases have also occurred frequently, especially vibriosis, which has a long onset time and high morbidity and mortality, causing serious economic losses to fishery production. . Long-term use of traditional antibiotics and other drugs will cause adverse consequences such as multi-drug resistance of bacteria, drug residues, and environmental damage. [0003] In addition, the deterioration of water bodies is one of the main factors leading to the outbreak of aquaculture diseases and the decline of production. Especially in recent years, with the rapid development of the a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A23K1/16A01P1/00C12R1/07A23K10/18
Inventor 张庆华王娟封永辉郭婧宋增福陈彪张永华
Owner SHANGHAI OCEAN UNIV
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