Recombinant low-virulent vaccine strain of chicken infectious bursal disease viruses (IBDV) and application thereof

A technology of bursal disease and chicken infectivity, which is applied in the fields of application, antiviral agents, virus antigen components, etc., can solve the problems of not being able to meet the emergency prevention and control of sudden outbreaks, and the method of domesticating vaccine strains is time-consuming and laborious, and achieves a mental state Good, good immunogenicity, good reproducible effect

Active Publication Date: 2011-01-05
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional domestication method of passaging weakened vaccine strains is time-consuming and laborious, and cannot meet the needs of emergency prevention and control of sudden outbreaks

Method used

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  • Recombinant low-virulent vaccine strain of chicken infectious bursal disease viruses (IBDV) and application thereof
  • Recombinant low-virulent vaccine strain of chicken infectious bursal disease viruses (IBDV) and application thereof
  • Recombinant low-virulent vaccine strain of chicken infectious bursal disease viruses (IBDV) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Design, rescue, identification and preliminary evaluation of immune efficacy of recombinant attenuated vaccine strain rGtHLJVP2

[0036] 1 Materials and methods

[0037] 1.1 Materials

[0038] 1.1.1 Viruses

[0039] HLJ-0504, vvIBDV [purchased from Harbin Institute of Veterinary Medicine, Chinese Academy of Agricultural Sciences (external)]; Gt, IBDV attenuated vaccine strain, by Harbin Veterinary Institute poultry immunosuppressive disease research group (the inventor's research group) will The vvIBDV Gx strain was attenuated and preserved by continuous passage. For specific methods, please refer to relevant literature (Wang X M, Zeng X W, Gao H L, et al. Changes in VP2 generating the attenuation of very virulent infectious bursal disease Virus strain Gxisolated in China. Avian Dis. , 2004, 48:77-83; Wang, X., Zhang H., Gao H., et al.Changes in VP3 and VP5 genes during the attenuation of very virulent infectious bursal disease virus strain Gx isolated in Ch...

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Abstract

The invention discloses a recombinant low-virulent vaccine strain of chicken infectious bursal disease viruses (IBDV) and application thereof. In the invention, a major protective antigen gene VP2 of an epidemic superhigh virulent strain is cloned, the nucleotide of the gene VP2 is modified by mutation and then used for replacing a corresponding segment of a Gt genome of a low-virulent strain of the IBDV, so that the infectious clone of a recombinant genome of the IBDV is constructed, and the recombinant low-virulent vaccine strain is saved and identified by using an IBDV reverse genetic operation system. The microbial collection number of the vaccine strain is CGMCC No.3749. The recombinant low-virulent vaccine strain of the invention has high replicability, genetic stability and safety. The immune effect of the low-virulent vaccine strain of the invention is as good as that of the medium-virulent vaccine strain, but is superior to that of the low-virulent vaccine strain. The biological safety of the low-virulent vaccine strain of the invention is superior to that of the medium-virulent vaccine strain. As the vaccine strain, the recombinant low-virulent vaccine strain of the invention has the characteristics of high efficiency and low toxicity, is a good candidate vaccine strain and can be used for controlling chicken infectious bursal disease.

Description

technical field [0001] The invention relates to a recombinant attenuated vaccine strain, in particular to a recombinant attenuated vaccine strain of chicken infectious bursal disease virus expressing the main protective antigen protein VP2 of epidemic strains, and the invention also relates to the preparation of the recombinant attenuated vaccine strain The invention relates to the application in the preparation of chicken infectious bursal disease biological products, belonging to the field of prevention and control of chicken infectious bursal disease. Background technique [0002] Chicken infectious bursal disease (Infectious Bursal Disease, IBD) is caused by chicken infectious bursal disease virus (Infectious Bursal Disease Virus, IBDV). Fatal infectious diseases are listed as "important diseases affecting society and economy" by the World Organization for Animal Health (OIE). The disease first broke out in the United States in 1957, and is now widespread in Europe, Sou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/40A61K39/12A61P31/14G01N33/569C12R1/93
Inventor 王笑梅祁小乐高玉龙高宏雷秦立廷王永强高立
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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