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Application of inhibin recombinant fusion protein to preparing medicines for promoting oestrus and hybridization of sows

A technology of fusion protein and sow estrus, which is applied in the direction of drug combination, pharmaceutical formula, peptide/protein composition, etc., can solve the problems of low breeding conception rate, low estrus performance, and increased return rate, so as to increase piglet size , Successful mating and normal conception improvement, the effect of a simple method

Inactive Publication Date: 2011-08-31
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] An object of the present invention is to overcome the deficiencies in the prior art in arousing estrus and promoting mating of anestrous sows, aiming at low estrus performance or low estrus performance of anestrous senior sows and anestrous gilts caused by various reasons, especially summer heat stress. Provide a new application of inhibin recombinant fusion protein to solve problems such as lack of anestrus, low mating conception rate or increased rate of return to oestrus

Method used

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  • Application of inhibin recombinant fusion protein to preparing medicines for promoting oestrus and hybridization of sows
  • Application of inhibin recombinant fusion protein to preparing medicines for promoting oestrus and hybridization of sows
  • Application of inhibin recombinant fusion protein to preparing medicines for promoting oestrus and hybridization of sows

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of porcine immunosuppressant vaccine

[0041] (1) Cloning of porcine inhibin coding gene cDNA:

[0042] a. According to the porcine inhibin gene sequence published in GenBank (Genbank number: NM_214189), design the specific upstream primer I1 and downstream primer I2 of the gene. The upstream primer I1 (5'-ATGTGGCCTCAGCTGCTCCTCTTGC-3') corresponds to the 1st to 25th nucleotides of the porcine inhibin gene coding region; the downstream primer I2 (5'-TTAGATGCAGGCACAGTGCTGGGTG-3') corresponds to the porcine inhibin gene coding region Nucleotides 1071-1095. The full length of the porcine inhibin gene is 1095 bp, and contains the complete coding region sequence of the inhibin α subunit gene.

[0043] b. Take the ovary tissue of Blue Tang pig, place it in 1ml guanidine thiocyanate phenol solution (Trizol solution, purchased from Invitrogen Company) for homogenization, then use chloroform to remove protein, centrifuge for 15 minutes, take out the supernat...

Embodiment 2

[0060] Embodiment 2 application experiment

[0061] 18 senior sows with 4 or 5 litters (average parity 4.7) were selected, because the last farrowing occurred in summer, and after weaning, the high temperature in summer caused long-term lack of estrus (the average empty period was 27 days) ), were randomly divided into 2 groups, 9 in each group, called group I and group C respectively. Among them, sows in group I were immunosuppressin recombinant fusion protein oil emulsion vaccine, and group C were injected with physiological saline adjuvant at the same time.

[0062] Immunization program:

[0063] The sows in group I were injected with inhibin recombinant fusion protein oil emulsion vaccine every 21 days. For the first time, 1 mL of oil emulsion vaccine containing 0.5 mg of recombinant porcine inhibin fusion protein was used, and for the second immunization, 1 mL containing 0.25 mg Oil emulsion vaccine of recombinant porcine inhibin fusion protein.

[0064] The test resul...

Embodiment 3

[0070] Select 20 gilts aged 8 months without estrus, and divide them into 2 groups at random, each with 10 gilts, called group A and group B respectively. Among them, the immunosuppressin recombinant fusion protein oil emulsion vaccine was injected into the backup pigs of group A, and the backup pigs of group B were injected with physiological saline adjuvant at the same time.

[0071] Immunization program:

[0072] The reserve pigs in group A were injected with inhibin recombinant fusion protein oil emulsion vaccine every 21 days. For the first time, 1 mL vaccine containing 0.5 mg recombinant porcine inhibin fusion protein was used, and for the second immunization, 1 mL containing 0.25 mg inhibin recombinant fusion protein was used. vaccine.

[0073] The experimental results showed that the accumulative estrus rate of 10 gilts treated with immunosuppressant within 50 days was 60%, while that of the control group was only 30%. image 3 shown.

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Abstract

The invention discloses an application of an inhibin recombinant fusion protein to preparing medicines for promoting oestrus and hybridization of sows. When inhibin is used for the sows: 0.5-1mg of the inhibin recombinant protein is used for initial immunization; and after 15-21 days, 0.25-0.5mg of the inhibin recombinant protein is used for second immunization. The immune inhibin can increase the estrus rate and the litter sizes of the sows. The invention has obvious effects on overcoming anestrus of the sows, caused by heat stress in summer, promoting the breeding activities of breeding sows, and particularly on promoting oestrus of breeding sows and back-up sows and increasing litter sizes. Thus, the non-productive time of the breeding sows is shortened, the breeding performance of thebreeding sows is improved, and the economic benefit of sow raising is increased.

Description

technical field [0001] The invention belongs to the new technology field of immune regulation for regulating pig reproductive activities, and specifically relates to the application of an inhibin recombinant fusion protein in the preparation of drugs for overcoming the adverse effects of summer heat stress and promoting estrus performance and mating of sows. . Background technique [0002] In the process of intensive and industrialized development of animal husbandry, the production efficiency of animal husbandry has been greatly improved due to the application of genetic breeding technology, feed compounding technology, disease prevention technology, environmental sanitation technology and modern enterprise management technology. improve. However, in each production link, the reproductive performance of animals has not improved in the past few decades. It is mainly reflected in the phenomenon of low or lack of estrus performance, low breeding conception rate or increased ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/00A61K38/17C12N15/09C07K19/00A61P15/08
Inventor 施振旦黎敏义陈倩
Owner SOUTH CHINA AGRI UNIV
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