Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Plant endophyte and application thereof

A technology of plant endophytic bacteria and bacterial liquid, applied in plant growth regulators, plant growth regulators, applications, etc., to achieve the effect of increasing zinc enrichment in grains, simple operation, and reduced investment

Active Publication Date: 2013-07-31
ZHEJIANG UNIV
View PDF1 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies in this area at present. In the actual agricultural production, it can be considered to use endophytic bacteria to make inoculum or soilless mixed inoculum for seed dressing to improve the yield and quality of crops.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plant endophyte and application thereof
  • Plant endophyte and application thereof
  • Plant endophyte and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Isolation of Enterobacteriaceae CGMCC7381

[0035] (1) culture medium

[0036] DF medium: every liter of culture medium contains KH 2 PO 4 4g, Na 2 HPO 4 6g, MgSO 4 ·7H 2 O0.2g, glucose 2g, sodium gluconate 2g, citric acid 2g, (NH 4 ) 2 SO 4 2 g, 0.1 mL each of component 1 and component 2 solutions, 15 g of agar, the solvent is water, pH 7.2, and sterilized by high pressure steam (121°C, 20 min).

[0037] Among them, component 1: H 3 BO 3 10mg, MnSO 4 ·H 2 O11.19mg, ZnSO 4 ·7H 2 O124.6mg, CuSO 4 ·5H 2 O78.22mg, MoO 3 Dissolve 10mg in 100mL sterilized distilled water and store at -4°C;

[0038] Component 2: FeSO 4 ·7H 2 O100mg was dissolved in 10mL sterilized distilled water and stored at -4°C.

[0039] DF+ACC medium: Add 3mM ACC to each liter of DF medium.

[0040] LB liquid medium: Each liter of culture medium contains 10 g of tryptone, 5 g of yeast extract, and 10 g of NaCl, the solvent is water, pH 7.0, and sterilized by high pressure ...

Embodiment 2

[0045] Identification of embodiment 2SaCS20 bacterial strain

[0046] (1) Observation of colony and cell morphology of SaCS20 strain

[0047] Bacterial Morphological Study: Gram-stained bacteria were observed under an optical microscope with a 16×100 oil lens.

[0048] Research on the internal structure of the bacteria: Ultra-thin sections were made on the bacteria, and the internal structure of the bacteria was observed with a transmission electron microscope.

[0049] On solid LB medium, the size of the SaCS20 strain is 2-5 mm; round, with neat edges; smooth, raised, opaque surface.

[0050] (2) Molecular biology identification of SaCS20 strain

[0051] Inoculate the preserved SaCS20 strain into LB liquid medium, cultivate overnight at 30°C on a constant temperature shaker at 180r / min, and extract the obtained bacterial liquid with Shanghai Sangon Biobacterial DNA Extraction Kit to amplify the 16S rDNA of the strain. After purifying the obtained PCR products, BGI was entr...

Embodiment 3

[0062] Example 3 Preparation of Enterobacteriaceae CGMCC7381 Bacteria

[0063] (1) Activation of bacteria

[0064] The original species of Enterobacter CGMCC7381 preserved at low temperature was inoculated in LB slant medium for activation culture, and placed in a constant temperature incubator at 37°C for 22 to 24 hours to obtain a slant of bacteria;

[0065] (2) Expansion of training

[0066] Put the activated strains into LB liquid medium for propagation, culture at 30°C, 180r / min constant temperature shaker, shake to the logarithmic phase, obtain the bacterial liquid, centrifuge the bacterial liquid, discard the supernatant, and use sterile Wash the precipitate with water for 3 times, suspend the precipitate with a phosphate buffer solution with a pH value of 7.0, and obtain a bacterial suspension containing Enterobacter CGMCC7381, and the bacterial suspension needs to be OD 600 Adjust to 0.8~1.0.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a plant endophyte with a category name of Enterobacter sp. and a complete name of Enterobacter sp. SaCS20. The strain is preserved at China General Microbiological Culture Collection Center (CGMCC) on March 28th, 2013, and has a preservation number of CGMCC NO: 7381. The invention also discloses the application of the plant endophyte in promoting paddy rice growth, improving paddy rice yield, or promoting trace element zinc accumulation in paddy rice. The Enterobacter sp. CGMCC 7381 provided by the invention is separated from the root system of a zinc hyperaccumulator sedum alfredii. The plant endophyte has no pathogenicity upon paddy rice, and can be successfully colonized into paddy rice root systems. With the plant endophyte, paddy rice growth can be promoted, rhizosphere soil available zinc content can be increased, and grain zinc enrichment can be promoted. The application provided by the invention is simple to operate, and is safe and economical. With the plant endophyte and the application, soil micronutrient fertilizer investment can be reduced, and crop yield can be increased.

Description

technical field [0001] The invention belongs to the technical field of rice microelement bioaugmentation, and in particular relates to a plant endophyte and its application. Background technique [0002] Zinc is closely related to human production and life activities. Zinc is an essential micronutrient element for plant growth and an important nutrient element necessary for the human body. Zinc has important physiological functions and nutritional value for the human body. Under normal circumstances, there are nearly 200 kinds of enzyme activities in the body related to zinc, so many enzyme activities are affected when the human body is deficient in zinc, resulting in a variety of metabolic disorders, which in turn affect human health. Studies have shown that zinc deficiency can cause short stature and skin disorders, especially for pregnant women and children. [0003] Zinc in the edible part of food crops is the main way for the human body to obtain zinc. Due to insuffic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01
CPCY02A40/10
Inventor 杨肖娥王玉艳张新成董兰学冯英韦燕燕高俊
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com