Bacillus methylotrophicus and application thereof

A methylotrophic, bacillus technology, applied in the direction of applications, bacteria, fungicides, etc.

Active Publication Date: 2015-11-04
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no report on the use of methylotrophic

Method used

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  • Bacillus methylotrophicus and application thereof
  • Bacillus methylotrophicus and application thereof
  • Bacillus methylotrophicus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Isolation and Preservation of Bacillus methylotrophicus SZ-3 and SZ-81

[0067] The strain was isolated from the soil within 30 cm around the rhizosphere of perennial ginseng in the ginseng cultivation base of Wanliang Town, Fusong County, Jilin Province. The above soil samples were collected, air-dried and sieved. Weigh 10 g of the sample, put it into a conical flask filled with glass beads and 90 mL of sterile water, shake it fully for 10-30 minutes, mix the sample with sterile water evenly, and prepare a sample suspension, and let it stand for 5 minutes. Take 1mL of supernatant under sterile conditions, add 9mL of 0.05% SDS aqueous solution (sodium dodecyl sulfate aqueous solution), keep warm at 40°C for 20min, take 1mL, add 9mL of sterile water, and make 10 -3 、10 -4 、10 -5 diluent. Pipette 100 μl of each dilution and add it to beef extract peptone (NB) plate, apply the plate dilution method evenly, repeat each treatment 3 times, and incubate in a 34°C incubator ...

Embodiment 2

[0071] Inhibitory Effects of SZ-3 and SZ-81 Strains on the Growth of Pathogens of Ginseng Fungal Diseases

[0072] The antagonism of strains SZ-3 and SZ-81 to ginseng fungal diseases were determined by the filter paper method: the activated ginseng pathogenic bacteria colony was made into a cake of the corresponding size with a puncher with a diameter of 8 mm, and aseptically inoculated into potatoes Glucose agar (PDA) plate (diameter 90mm) in the center, while four pieces of sterilized filter paper discs with a diameter of 1cm are glued to the four corners at a distance of 25mm from the center of the plate. Bacterial strains SZ-3 and SZ-81 were respectively made into bacterial suspension (concentration was about 10 8 cfu / mL), in which 3 pieces of filter paper discs were spotted with 20 μL of bacterial suspension, and 1 piece of filter paper discs was spotted with 20 μL of sterile water, as a treatment group, and each treatment was repeated 3 times; The 4 filter paper disks a...

Embodiment 3

[0080] Antagonistic activity of methylotrophic bacillus SZ-3 and SZ-81 against ginseng pathogens

[0081] Adopt the Oxford cup method to measure respectively the antagonistic activity of bacterial strain SZ-3 and SZ-81 bacterial strain to ginseng fungal disease pathogen: SZ-3 and SZ-81 bacterial strain are respectively made into after bacterial suspension and collect (preparation method is the same as embodiment 2), Centrifuge at 12,000 r / min for 20 min at 4°C, collect the supernatant and filter through a 0.22 μm microporous membrane, and store the filtrate at 4°C for future use. Inoculate a ginseng germ cake with a diameter of 8mm in the center of the PDA plate under sterile conditions, then place 4 sterile Oxford cups at 4 symmetrical corners about 25mm away from the center of the plate, and add 100 μl of filtrate dropwise to each cup. Repeat 3 times, and measure the width of the inhibition zone after culturing at 28°C for 5 days.

[0082] The results showed that the inhibi...

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Abstract

The invention provides bacillus methylotrophicus SZ-3 and SZ-81, with collection numbers of CGMCC No.8275 and CGMCC No.8276 respectively. The bacillus methylotrophicus SZ-3 has obvious antagonistic effects on pathogenic bacteria causing root rot, black spot and sclerotinia sclerotiorum of ginseng. The bacillus methylotrophicus SZ-81 has obvious antagonistic effects on pathogenic bacteria causing epidemic diseases, root rust rot and black spot of panax ginseng. The invention also provides an application of the bacillus methylotrophicus SZ-3 and SZ-81 to control of fungal diseases of plants or an application to preparation of microbial preparations for controlling fungal diseases of plants.

Description

technical field [0001] The present invention relates to methylotrophic bacillus and application thereof. Background technique [0002] Ginseng (Panax ginsengC.A.Mey) is a perennial root-negative dicotyledonous medicinal plant of Araliaceae Panax. It is a precious medicinal material in my country and has the reputation of "the king of herbs". Ginseng is planted in large areas in China, South Korea, North Korea, Russia and other countries. Among them, Northeast China is the main production area of ​​ginseng and has become one of the important pillar industries in the local area. The long-term artificial planting of ginseng, and the difficulty of breed selection lead to the degradation of ginseng germplasm, serious diseases, poor quality and low yield. Extensive use of chemical pesticides leads to pesticide residues and environmental pollution, which reduces the safety and commodity value of ginseng medicinal materials. Disease prevention and control has become one of the maj...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00C12R1/07
Inventor 杨利民孙卓韩梅张永刚刘翠晶
Owner JILIN AGRICULTURAL UNIV
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