Biocontrol bacillus subtilis WJ-1 for rice sheath blight, microbial inoculum and application thereof

A technology of Bacillus subtilis and rice sheath blight, applied in the direction of application, biocide, fungicide, etc., to achieve obvious control effect, simple production and use

Inactive Publication Date: 2010-10-13
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Biocontrol bacillus subtilis WJ-1 for rice sheath blight, microbial inoculum and application thereof
  • Biocontrol bacillus subtilis WJ-1 for rice sheath blight, microbial inoculum and application thereof
  • Biocontrol bacillus subtilis WJ-1 for rice sheath blight, microbial inoculum and application thereof

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Embodiment 1

[0034] Example 1: Isolation and identification of Bacillus subtilis WJ-1 and research on its bacteriological characteristics

[0035] 1. Isolation and identification of Bacillus subtilis WJ-1

[0036]A biocontrol bacterium Bacillus subtilis (Bacillus subtilis) WJ-1 suitable for the prevention and treatment of rice sheath blight of the present invention was isolated and screened on the vegetable garden soil of Huazhong Agricultural University campus in Shizishan District, Wuchang, Wuhan City, Hubei Province. Its separation and identification methods are carried out according to the method reported (Edited by Zhou Deqing, "Microbiology Experimental Technology Manual", Shanghai Science and Technology Press, 1986 edition), which is a routine microbial isolation and identification method. The bacterial strain has been submitted to the Chinese Type Culture Collection Center (CCTCC) located in Wuhan University, Wuhan City, Hubei Province for patent procedure preservation on March 30,...

Embodiment 2

[0049] Embodiment 2: the preparation of bacillus subtilis WJ-1 bacterial agent

[0050] (1) Activation culture of the WJ-1 strain: First, the WJ-1 strain with the deposit number CCTCC NO: M2010069 was activated on a PDA medium (formulation as described in Example 1) plate at 33° C. for 1 day.

[0051] (2) Liquid culture of the WJ-1 strain: Inoculate the single colony prepared in step 1 into a 500ml Erlenmeyer flask containing 200ml of PDB (the formula is the same as PDA, except that no agar is added), 180r / min, 33°C shaking culture for 48h, that is It is fermentation broth A, wherein the concentration of Bacillus subtilis WJ-1 is 1.5×10 10 cfu / ml.

[0052] (3) Preparation of WJ-1 bacterial agent: Centrifuge the fermentation broth prepared in step 1 at 6,000r / min for 20min at 4°C, take the centrifuged supernatant, and filter it with a common bacterial filter (pore size 0.22μm) supernatant to obtain a sterile fermentation filtrate (abbreviated as fermentation broth B). Wash t...

Embodiment 3

[0053] Embodiment 3: In vitro and indoor living test of bacillus subtilis WJ-1 bacterial agent for controlling rice sheath blight

[0054] (1) Indoor in vitro inoculation control test:

[0055] The rice sheath blight strain WH-1 of the present invention was activated on a PDA medium plate at 28° C. (for the method, refer to the method reported by Xie et al., 2008). Cut 7.5 cm leaves of rice seedling stage (5-leaf stage), place them in a sterile glass petri dish (diameter 9 cm), put absorbent paper in the petri dish to keep moist, and each dish has 3 leaves. Setup processing includes:

[0056] ① Spray clean water, fermentation broth A, fermentation broth B and bacteria fluid C until there is a layer of liquid film on the surface of the rice leaves, and after 12 hours, connect the activated 5mm rice rhizoctonia (WH-1) bacteria block to the rice leaves , a distance of about 2cm;

[0057] ②Spray clear water, fermentation broth A, fermentation broth B, and bacteria fluid C until...

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Abstract

The invention belongs to plant disease biocontrol technical field, and relates to separation identification on bacteria for biocontrol of rice sheath blight, microbial inoculum containing the bacteria or a fermentation product thereof, and control on rice sheath blight. In the invention, the bio-control bacteria-a bacillus subtilis WJ-1 bacterial strain for controlling rice sheath blight is obtained by separating and screening and then preserved in China Center for Type Culture Collection, wherein, the preservation number is CCTCC No: M2010069. The invention further discloses a preparation method of the biocontrol microbial inoculum and an application of three bacillus subtilis WJ-1 microbial inoculum for controlling rice sheath blight. The biocontrol microbial inoculum of the invention has good biocontrol effect on plant fungal diseases such as rice sheath blight and the like.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to the isolation and identification of a bacterium used for the biological control of rice sheath blight, a bacterial agent containing the bacterium or a fermentation product thereof, and the prevention and control of rice sheath blight. Background technique [0002] Rice sheath blight occurs in all rice areas in the world, and it is more serious in my country mainly in the rice areas of South China and the Yangtze River Basin. In the 1980s, it broke out in a large area in rice planting areas and developed into a kind of rice disease in my country. Main diseases; after the 1990s, the occurrence of the disease was more rampant, and the proportion of the diseased area in southern rice areas generally exceeded more than 50%, becoming the main obstacle of rice production in my country, and the diseased area and damage loss ranked first in rice dise...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/125
Inventor 谢甲涛游景茂姜道宏付艳苹程家森
Owner HUAZHONG AGRI UNIV
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