Biological pesticide using psoralidin to prevent and control plant fungal diseases

A technology for plant fungal diseases and psoralen, which is applied in the directions of plant growth regulators, chemicals for biological control, biocides, etc. Good inhibitory activity, high efficacy and broad bactericidal spectrum

Active Publication Date: 2008-07-09
沈阳同祥生物农药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term use of some conventional varieties, pathogenic bacteria have developed drug resistance, resulting in an increase in the amount of field medication year by year, which has brought irreversible negative effects on human living environment and health.

Method used

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  • Biological pesticide using psoralidin to prevent and control plant fungal diseases
  • Biological pesticide using psoralidin to prevent and control plant fungal diseases

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0015] Example 1: Using acetone or dimethyl sulfoxide to dissolve Psoralidin (Psoralidin) to prepare a 10g / L crude extract mother liquor. Add an appropriate amount of crude extract mother liquor to PDA medium at 50℃, mix well, and prepare the final concentration of crude extract to be 0.12mg / L, 0.6mg / L, 3mg / L, 15mg / L, 75mg / L. The drug-containing medium is added to the PDA medium with an equal volume of acetone or dimethyl sulfoxide as a control, and then poured into a petri dish with a diameter of 90 mm to make a flat plate for use. A 6mm diameter hole punch was used to cut the edge of each test pathogen colony that had been cultured on a PDA plate at 28°C for 7-12 days, and transplanted it to the center of the above-mentioned crude extract plate and control plate containing different concentrations. This treatment was repeated 3 times, cultured at 28°C for 7-12 days, and the colony diameter was measured. The diameter of each colony is measured in the direction of the intersecting...

example 2

[0018] Example 2: The test method is the same as that of Example 1. The inhibitory effects of the six original medicines of psoralen, thiram, Yintai, thiophanate methyl, and carbendazim on the growth of the mycelium of apple rot fungi are as follows:

[0019] Inhibition of 5 kinds of medicaments on mycelium growth of apple rot disease fungus

[0020] Inhibition

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Abstract

The invention provides a biological pesticide by using psoralidin to control plant fungous disease. The invention relates to a plant used biological pesticide, in particular to biological pesticide by using psoralidin to control plant fungous disease. Compound is dried Guzhi seed 5Kg. The compound is quenched and extracted four times by using 70 percent of industrial ethanol. After lixivium is decompressed and condensed, ethanol extract is gained. Water is added and mixed with the ethanol extract. Mixture is extracted by using petroleum ether and ethyl acetate in turn. 100g of ethyl acetate extraction part extract is taken, and processed chromatography over again by silica gel column, and processed gradient elution by petroleum ether-ethyl acetate and are separated, and then psoralidin (50mg) is gained (the ratio between the petroleum ether and ethyl acetate is 4 to 1). The invention can restrain common plant pathogen, like apple decay pathogen, apple macrophoma kawatsukai, pyricularia grisea, rice sheath blight fungus, cucumber fusarium wilt, wheat scab pathogen, cucumber anthracnose, phytophthora parasitica and so on.

Description

Technical field [0001] The invention relates to a biological pesticide for plants, in particular to a biological pesticide for preventing and treating plant fungal diseases with psoralen. Background technique [0002] According to statistics, my country’s rice planting area is 28.82 million hectares (2001), wheat planting area is 21.99 million hectares (2003), apple area is 2.3 million hectares, and vegetable area is increasing year by year. Apple rot, apple ring pattern, rice blast, rice sheath blight, cucumber wilt, wheat gibberella, cucumber anthracnose, and Phytophthora capsici are common plant diseases in the production of the above-mentioned crops and fruits and vegetables. Chemical pesticides are mainly used for the prevention and control of the above-mentioned diseases. For example: 50% carbendazim, 70% thiophanate methyl, Intime, etc. However, due to the long-term use of some conventional varieties, the pathogens have become resistant to drugs, which has caused the amoun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/00A01P3/00
CPCY02A50/30
Inventor 关丽杰邵红蔡丽丽邵双李海燕
Owner 沈阳同祥生物农药有限公司
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