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Paenibacillus kribbensis and its application

A technology of bacillus and strains, applied in the field of biotechnology prevention and control, can solve the problems of Paenibacillus Cliburnum with few antifungal bioactive substances, etc., and achieve strong broad-spectrum antibacterial activity, excellent control effect, and outstanding effect

Active Publication Date: 2014-04-30
JIANGXI TIANREN ECOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few research reports on the antifungal bioactive substances of Paenibacillus Kliben

Method used

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  • Paenibacillus kribbensis and its application
  • Paenibacillus kribbensis and its application
  • Paenibacillus kribbensis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1. Isolation and identification of Paenibacillus kribbensis TRCC82001CGMCC No.7996.

[0067] 1. Isolation of Paenibacillus kribbensis TRCC82001CGMCC No.7996: Paenibacillus kribbensis TRCC82001CGMCC No.7996 was isolated from the soil of the herbarium of South China Agricultural University. The method is as follows: collect soil samples from the herbarium of South China Agricultural University, weigh 1.0 g of soil, put it into a conical flask with 99 mL of sterile water, shake it evenly, and dilute 10 -2 soil suspension. Then use a ten-fold serial dilution to prepare 10 -3 、10 -4 、10 -5 、10 -6 、10 - 7 Dilution of soil dilution. Use a sterile scale to draw 0.1mL of soil dilutions of each concentration, inoculate them on the beef extract peptone agar medium plate with aseptic technique, spread evenly, and repeat 5 times for each concentration. After the inoculated plate was cultured at a constant temperature of 30 degrees Celsius for 2 to 5 days, a single co...

Embodiment 2

[0088] Example 2. Determination of antibacterial activity of Paenibacillus kribbensis TRCC82001CGMCC No.7996 and its metabolites.

[0089] 1. Paenibacillus kribbensis TRCC82001CGMCC No.7996 cells and

[0090] Thermostability of its antibacterial metabolites.

[0091] Get Paenibacillus kribbensis (Paenibacillus kribbensis) TRCC82001CGMCC No.7996, 30 degrees Celsius, 180r / min shaking culture 6 parts of 100 times of fermented liquid diluted 84 hours, wherein 5 parts are respectively in 40 degrees Celsius, 60 degrees Celsius, 80 degrees Celsius, Treat at 100 degrees Celsius and 120 degrees Celsius for 30 minutes; the other part is left untreated as a control group, and each treatment has 5 repetitions. Plates were respectively connected to do bacterial survival and antibacterial tests on rice blast, and the results are shown in Table 2 below. The results showed that the fermentation antibacterial metabolites were stable at a temperature below 100 degrees Celsius, and the bacteri...

Embodiment 3

[0099] Example 3. Greenhouse and field application test of aseptic fermentation broth of Paenibacillus kribbensis TRCC82001CGMCC No.7996.

[0100] 1. Greenhouse cultivation control test of Paenibacillus kribbensis TRCC82001CGMCC No.7996 aseptic fermentation broth:

[0101] The rice variety is Xinrongyou 08, which was purchased from Jiangxi Provincial Seed Company. After soaking and accelerating germination according to the rice pot test method, after the radicle grows to about 2 cm, select rice seedlings with the same growth and transplant them into plastic square pots for soil culture. When it grows to the 3-leaf stage, the rhizomes are inoculated with rice sheath blight fungus, and the disease incidence is observed at the same time. see attached result Figure 4 .

[0102] The test results showed that the leaves of the blank control group were covered with rice sheath blight mycelium after 3 days, the leaves withered and turned yellow after 5 days, and a large number of sc...

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Abstract

The invention discloses a Paenibacillus kribbensis, and a preparation method and an application thereof. The Paenibacillus kribbensis has a bacterial code name of TRCC82001, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCCNo.7996. The above bacterial strain has the advantages of good production property, good metabolite antimicrobial effect, and strong activity against rice blast and sheath blight, and a solution obtained after diluting a broth subjected to antibiotic treatment to 100 times has an inhibition effect of above 80% on the rice blast and sheath blight. The bacterial strain and its metabolites have a broad antimicrobial spectrum, can be used for preparing biocontrol preparations for the broad spectrum activity inhibition of pathogenic fungi to control the fungal diseases of plants.

Description

technical field [0001] The invention belongs to the field of biotechnology prevention and control, and in particular relates to the preparation of a strain of Paenibacillus kribbensis and its bacterial agent and its application in inhibiting pathogenic fungi and preventing and controlling plant fungal diseases. Background technique [0002] Plant diseases are factors that affect plant yields in modern agricultural production. According to FAO data, the annual losses caused by plant diseases in the world account for 10%-15% of the total crop production. With the improvement of the agricultural planting industrial structure Adjustment, the control and prevention of crop diseases have attracted more and more attention. Plant diseases caused by phytopathogenic fungi are the first of the three major plant diseases, accounting for 70% of all plant diseases. The main diseases of some crops such as rice blast, rice sheath blight; wheat rust, wheat powdery mildew; maize large and sm...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/01
Inventor 梁小文王根豪陈晓燕廖美德范志金周立峰李肖宇马忠岩曾繁富肖筱成熊建生曾升华吴俊杰钱凤朱湘盛
Owner JIANGXI TIANREN ECOLOGY
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