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Bactericide and application thereof

A fungicide and sclerotinia technology, which is applied in the application field of Lansiumamide B or its salt as a fungicide, can solve the problems that the activity of the compound has not been significantly improved, and the antibacterial spectrum of Lansiumamide B has not been determined.

Inactive Publication Date: 2017-11-07
闫合
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Wan Shuqing et al. used the mycelial growth rate method to measure the antibacterial activity of Lansiumamide B against mango anthracnose and banana Fusarium wilt, and found that at a concentration of 0.08 mg / mL, the compound inhibited the mycelial growth of mango anthracnose and banana The rates were 83.33% and 60.78% respectively; Wan Shuqing et al. artificially synthesized 20 kinds of cinnamic amide compounds using Lansiumamide B as a template, and measured the in vitro antibacterial activity of the obtained compounds against mango anthracnose bacteria, and found that the synthesized compounds had no activity. see significant improvement
[0005] Although Wan Shuqing et al. determined the antibacterial activity of Lansiumamide B and its derivatives against mango anthracnose and banana wilt and published it as an article, they did not determine the antibacterial spectrum of Lansiumamide B or propose any substantive product formula

Method used

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  • Bactericide and application thereof
  • Bactericide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Lansiumamide B bioactivity assay in vitro

[0029] The antibacterial activity of the compound Lansiumamide B was determined by the mycelia growth rate method. A total of 22 pathogenic bacteria were selected. The tested bacterial species and the inhibition rate of each compound at a concentration of 100ug / ml are listed in Table 4-1. Prepare the test sample solution 400ug / ml with sterile water. Melt the culture medium and cool it down to about 50°C, mix 20ml of the sample solution to be tested with 20ml / bottle of PDA medium respectively to make a drug-containing medium of corresponding concentration, shake well, and quickly pour into three 9cm-diameter In the petri dish, after it is solidified, the diameter of the colony is 0.8cm, and the outer bacterium block of the apple anthracnose pathogenic bacterium purified by monospore is placed in the center of the petri dish (mycelia face down), and an equal volume of sterile water is added to The blank control an...

Embodiment 2

[0035] Embodiment 2: Lansiumamide B pot bioactivity assay

[0036] The control of wheat powdery mildew and cucumber downy mildew was carried out by pot experiment. The specific method is as follows: fill the sterile soil to three-fifths of the total volume of the 220mL paper cup, and sow wheat. Eighteen days after sowing, when the wheat grew to two leaves, the test was carried out. The test compound Lansiumamide B was treated with 500mg / L, and water control and 15% triadimefon wp standard drug control were set at the same time. The pesticides were applied by conventional spraying and root irrigation (10 mL per pot), inoculated by the spore shake-off method, and the treated wheat was cultured in a greenhouse. For the protective effect test, the wheat seedlings were first sprayed or root-irrigated, and then inoculated 24 hours later. For the therapeutic effect test, inoculate powdery mildew bacteria on wheat seedlings first, and then spray or irrigate the roots after 24 hours...

Embodiment 3

[0040] Example 3: Determination of Lansiumamide B and other fungicides in vitro biological activity

[0041] This embodiment adopts mycelia growth rate method to measure, and the method is the same as that described in Example 1. 1 in the sample is Lansiumamide B, and the concentration is listed as the concentration of Lansiumamide B + the concentration of the compound drug. The results of the antibacterial rate test are shown in Table 4-3.

[0042] Table 4-3 Inhibition rate of Lansiumamide B and representative fungicides against seven pathogenic bacteria

[0043]

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Abstract

The invention discloses an application of lansiumamide B or salt thereof as a bactericide. The lansiumamide B is amide-type alkaloid and is obtained by separation from wampee stone. The bactericide has broad-spectrum bactericidal activity to multiple plant fungous diseases, can be used individually or mixed with other medicines for treating multiple plant fungous diseases, and has a particularly better treatment effect on paddy rice sheath blight, rape sclerotinia rot, strawberry gray mold. The lansiumamide B can also be used as seed treatment, medical fertilizer, and fresh-keeping of fruits, vegetables and fish.

Description

technical field [0001] The invention belongs to the field of agricultural fungicides, and in particular relates to the application of Lansiumamide B or a salt thereof as a fungicide. Background technique [0002] Phytopathogens are very harmful to the growth of plants and cause huge losses to agriculture every year. In order to prevent the occurrence of plant diseases and reduce the losses caused by them, a large number of antibacterial and bactericidal active compounds have been reported and applied. Due to factors such as drug resistance, the research and development of new fungicides has always been the focus of attention of various pesticide companies and researchers. [0003] Wamp skin [scientific name: Clausena lansium (Lour.) Skeels] Rutaceae, native to southern China, is one of the fruits, rich in vitamin C, sugar, organic acids and pectin. Both the peel and the core of the yellow skin can be used as medicine. It has the effect of digestion and phlegm, regulating q...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/18A01P3/00A01C1/06C05G3/00A23B7/154A23B7/16A23B4/20A23L3/3526
CPCA01N37/18A01C1/06A23B4/20A23B7/154A23B7/16A23L3/3526A23V2002/00C05B7/00C05G3/00
Inventor 闫合
Owner 闫合
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