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B. subtilis with effects of resisting disease and improving fruit quality and application thereof

A technology of Bacillus subtilis and pathogenic bacteria, applied in the field of microorganisms, can solve the problem of single control effect, and achieve the effect of reducing use, good broad-spectrum antibacterial effect, and broad antibacterial spectrum

Active Publication Date: 2019-08-27
SHANDONG BEE LAN BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above research background, the inventors believe that providing a strain with broad-spectrum antibacterial effect is beneficial to overcome the single defect of current biocontrol agents

Method used

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  • B. subtilis with effects of resisting disease and improving fruit quality and application thereof
  • B. subtilis with effects of resisting disease and improving fruit quality and application thereof
  • B. subtilis with effects of resisting disease and improving fruit quality and application thereof

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Experimental program
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Embodiment 1

[0050] 1. Screening and identification of strains

[0051] Soil samples were collected from Ningyang County, Shandong Province, and 1g of the sample was weighed and placed in 100ml of sterile water, incubated in a 30°C thermostat for 5-7 days with shaking, and the soil sample liquid was subjected to 10 5 -10 6 For multiple dilution, the dilution was spread on a plate containing NA solid medium, and cultured overnight at 30°C. The strains were isolated and purified from the plates where colonies grew, and stored in NA slant medium.

[0052] Observe the characteristics of the purified strain under a microscope (such as figure 1 , figure 2 ): Gram-positive straight rods in the shape of bacteria, 0.6 × 1.0-1.4 μm in size, with rounded ends, grouped singly or in pairs, forming endospores. Spores are ellipsoidal with a central or subapical orientation. The sporangia are not swollen. The colonies are dry, wrinkled, regular, creamy, 2-4 mm in diameter, with a flattened outline....

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PUM

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Abstract

The invention belongs to the technical field of microbiology, and particularly relates to B. subtilis with the effects of resisting disease and improving fruit quality and application thereof. A microbial preparation is adopted to increase crop yield and control the pest and disease problem, and can effectively reduce the harmful effects of traditional chemical agents on soil. The invention provides B. subtilis 21c and a compound preparation thereof. The strain has an antagonistic effect on plant fungal pathogenic bacteria and plant bacterial pathogenic bacteria, and especially has a significant control effect on plant root rot; the compound preparation is applied to greenhouse tomato experiment, and can increase fruit production by 15%; diseases occurring during tomato growth are effectively controlled, and the control effect is up to 80% or more; various tomato fruit indicators are improved, and lycopene content is significantly improved. While the quality of crops is improved, the harmful effect on the soil is reduced, and good promotion significance is achieved.

Description

technical field [0001] The disclosure relates to the technical field of microorganisms, in particular to the application of Bacillus subtilis 21c with disease resistance and fruit quality improvement effects and its composite bacterial agent in disease resistance and fruit quality improvement. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] In China, the losses caused by pests and diseases in the process of agricultural production account for more than 30% of the total agricultural output value every year. In order to recover these losses, chemical control, biological control, genetic control, physical control and other methods are widely used. Chemical control has the...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00A01P21/00C12R1/125
CPCC12N1/20C12R2001/125C12N1/205A01N63/10Y02A40/10
Inventor 单宝龙胡著然高国瑞孔德荣郭静刘晓晓艾米莉亚·卡米莉娅安娜·斯塔琳娜玛丽娜·迈瑞林肯斯尤里劳斯·库帕特索夫张文娟申小冉侯磊
Owner SHANDONG BEE LAN BIOTECHNOLOGY CO LTD
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