Rapeseed pseudomonas with plant growth promoting effect

A Pseudomonas rape, plant growth promotion technology, applied in the directions of plant growth regulators, plant growth regulators, botanical equipment and methods, etc., can solve the problems of high cost, secondary pollution, large engineering volume and the like, To achieve the best salt tolerance, good potassium solution, strong vitality effect

Active Publication Date: 2019-01-11
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The improvement of physical methods is mainly based on leaching and salt drainage, combined with measures such as deep plowing to improve soil, soil replacement, leaching, and sedimentation to achieve improvement effects; there are problems such as large engineering volume and high cost
Chemical method improvement is to apply some acidic salt substances, combined with the application of organic fertilizers to improve the soil physical properties of saline-alkali land; there are problems such as secondary pollution

Method used

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  • Rapeseed pseudomonas with plant growth promoting effect
  • Rapeseed pseudomonas with plant growth promoting effect
  • Rapeseed pseudomonas with plant growth promoting effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: Screening, isolation and identification of bacterial strains

[0068] 1. Treat the rhizosphere of native vegetation in saline-alkali land (salthorn grass, miscanthus, corn, etc.) collected from Ningxia, Shandong, etc.: carefully shake off the soil on the surface of the root system, and use sterilized soft water to clean the soil tightly attached to the rhizosphere surface. Gently brush the brush into sterile water to obtain the rhizosphere soil stock solution, use a disposable sterile syringe to sample, and after counting on the hemocytometer, dilute to 10 3 、10 4 、10 5 、10 6 Dilution, and then respectively coated on the beef extract peptone medium containing 1%, 5%, 10% NaCl (w / v). Incubate the petri dish upside down at 30°C for 1 to 4 days, pick different types of typical single colonies, purify them on the plate, and store them on a slant at 4°C.

[0069] The specific purification method is as follows: in an aseptic operating environment, use an inocul...

Embodiment 2

[0077] Example 2: Determination of the ACC Deaminase Ability of the Bacterial Strain YZX4

[0078] ACC is the direct premise of ethylene in plants, and ethylene, as an important plant hormone, has the functions of promoting fruit ripening, promoting leaf senescence, inducing adventitious roots and root hairs, and breaking seed dormancy. Under the stress of harsh environments such as drought, the vague metabolism of plant roots is enhanced, leaf maturation, aging and death are accelerated, and photosynthesis of leaves is reduced, thereby reducing crop yield.

[0079]The inventors found that the strain YZX8 can secrete ACC deaminase, degrade ACC into α-butyruvate and ammonia, reduce the concentration of ACC, and then promote the continuous secretion of ACC from the root system, thereby reducing the concentration of ACC and stress ethylene inside the plant, and reducing the concentration of ACC. Harmful environment to plants, promote the normal growth and development of plants. ...

Embodiment 3

[0096] Example 3: Determination of the ability of bacterial strain YZX4 to synthesize IAA

[0097] IAA, also known as indole acetic acid, can promote cell growth and differentiation, thereby promoting plant growth and development. The inventors found that strain YZX4 can secrete IAA, thereby promoting plant growth.

[0098] 1. Drawing of IAA standard curve: configure 3-IAA (3-indoleacetic acid) standard solution to 250 mg / L, dilute to 2.5, 5, 10 and 25 mg / L respectively, and mix with Salkowski reagent at a volume ratio of 1:5 Mix and place in the dark for 30 minutes at room temperature, respectively measure the absorbance of each concentration standard solution at a wavelength of 530nm. Taking IAA concentration as the abscissa, OD 530 The value is plotted on the ordinate to obtain the IAA standard curve. The result is as Figure 7 As shown, the standard curve is: y=0.0134x+0.0232.

[0099] 2. Inoculate bacterial strain YZX4 in 5mL beef extract peptone medium (liquid), cul...

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Abstract

The invention belongs to the application field of microorganisms, in particular to a rapeseed pseudomonas with plant growth promoting effect. The specific technical scheme is as follows: a strain of rapeseed pseudomonas is deposited in the General Microbial Center of China Microbial Culture Collecting Management Committee on July 30, 2018, and the deposit number is CGMCC No. 16170. The rapeseed pseudomonas has excellent salt tolerance, can maintain strong vitality and excellent ability of secreting plant hormones at high salt concentration, and can secrete chitinase and degrade chitin, thus having certain effect on controlling plant fungal diseases. The results show that the strain of Pseudomonas campestris has good salt tolerance, and can be used for controlling plant fungal diseases. Atthe same time, the bacterium also has good potassium and phosphorus solubilization effect, and can be practically used in saline-alkali soil improvement.

Description

technical field [0001] The invention belongs to the field of microorganism application, and in particular relates to a strain of Pseudomonas oleifera with plant growth-promoting effect. Background technique [0002] Soil salinization is one of the important reasons leading to high input, low output and low benefit in agricultural production. At present, my country is facing the dual pressure of population increase and resource reduction. How to better manage and utilize saline-alkali land is not only an urgent requirement to maintain the continuous operation of agricultural production, but also an important way out for the development of existing agricultural development potential in saline-alkali areas. [0003] Now, saline-alkali land improvement measures mainly include physical, chemical and biological methods. The improvement of physical methods is mainly based on leaching and salt drainage, combined with measures such as deep plowing to improve soil, soil replacement, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00A01P3/00C09K17/14C12R1/38C09K109/00
CPCA01N63/00C09K17/14C09K2109/00C12N1/205C12R2001/38
Inventor 李东孙晓莹刘晓风陈意超
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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