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Application of gingko endogeny Chaetomium globosum in prevention and control of plant pathogenic fungi

A technology for Chaetomium endophytes, phytopathogenic fungi, applied in microorganism-based methods, chemicals for biological control, applications, etc.

Inactive Publication Date: 2012-10-24
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a kind of endophytic fungus---Chaetomium globosum (Chaetomium globosum) NO.04 isolated and screened out from Ginkgo biloba for the shortage of unsafe chemical pesticides and less types of microbial source pesticides; the present invention Another purpose is to provide the application of using the bacterial strain and its metabolites to antagonize maize leaf rot, grape white rot, and rhizopus stoloniferum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: Isolation and screening of Chaetomium globosa NO.04

[0023] The present invention collected Ginkgo biloba leaves in Linyi, Shandong Province in June 2008, and obtained Chaetomium globosa NO .04, save.

[0024] Determination of the antibacterial activity of Chaetomium globosa No.04: inoculate a 9-cm-diameter piece of corn leaf rot fungus, grape white rot fungus, and rhizopus stoloniferum on the central side of the PDA plate, and spot the chaetomium at a distance of 4.5 cm from the fungus block Bacteria No.04, cultivated at a constant temperature of 28°C for 2-4 days, observe whether there is an antibacterial zone, and measure the size, set the treatment of No. powerful.

[0025] The inhibition rate was calculated by the following formula:

[0026] Inhibition rate (%)=(control group pathogenic bacteria growth radius-treatment group pathogenic bacteria growth radius) / (control group pathogenic bacteria growth radius-original bacteria cake radius) x 100%

...

Embodiment 2

[0028] Embodiment 2: the preparation method of chaetomium globosa NO.04 fermentation metabolite

[0029] Include the following steps:

[0030] (1) Activation culture of strains: move the isolated and preserved strain of Chaetomium globosa No.04 onto a potato dextrose PDA solid plate, and cultivate it at 28°C±1°C until the hyphae cover the entire petri dish. A diameter of 9cm is punched to obtain a fungus cake for inoculation;

[0031] (2) Fermentation culture: For every 1000mL of distilled water, 30g of oats, 20g of maltose, and pH 6.0-7.0 of the fermentation culture medium. Each 500mL triangular bottle contains 200mL of fermentation broth; after preparation, sterilize at 121°C for 20 minutes, cool to 40°C and pick out a bacterium cake with a diameter of 9cm under aseptic conditions. Fermentation culture 7-8d;

[0032] (3) Extraction of fermented product: After the fermentation is completed, the fermented liquid is collected by centrifugation, added with an equal volume of ...

Embodiment 3

[0033] Example 3: Inhibition of Chaetomium globosa NO.04 fermentation metabolites on corn leaf spot fungus, grape white rot fungus, and rhizopus stoloniferus

[0034] The fermentation metabolite of Chaetomium globosa NO.04 is the fermentation broth prepared in Example 2.

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PUM

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Abstract

The invention relates to an application of a strain of gingko endogeny Chaetomium globosum NO.04 in prevention and control of plant pathogenic fungi, and belongs to the technical field of biological prevention and control of plant diseases. A strain of the gingko endogeny Chaetomium globosum NO.04 and fermentation metabolites thereof in the present invention can be applied for prevention and control of northern corn leaf blight, Grapevine white rot, and tomato rhizopus fruit rot caused by plant pathogenic fungi. According to the present invention, a microbe strain is provided for prevention and control of northern corn leaf blight, Grapevine white rot, tomato rhizopus fruit rot, and other plant pathogenic fungi diseases on agriculture; and the provided fermentation metabolites can be applied in prevention and control of plant fungi diseases, and a new approach is provided for development of agricultural fungicides.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to the use of a strain of Chaetomium ginkgo no. stolonifer). Background technique [0002] The drug resistance of plant pathogens is a serious problem in the chemical control of plant diseases, which brings great difficulties to the control of diseases of various plants (such as agricultural crops, economic crops and horticultural crops, etc.). Due to the long-term and extensive use of chemical fungicides, especially systemic fungicides, the resistance of plant pathogens to them is very prominent. It is necessary to overcome the problem of drug resistance through integrated control, especially in combination with biological control, so as to effectively control plant pathogens. [0003] However, most of the current biological control bacteria are still in the initial stage of research and development, far from meeting the actual requirement...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P3/00C12P1/02C12R1/645
Inventor 刘金亮张桂珍潘洪玉秦建春曲晓艳莫华文张婧赢张艳华
Owner JILIN UNIV
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