One strain of streptonmyces lydicus and its application in treatment and prevention of plant's disease

A technology for Streptomyces lideni and plant fungal diseases, which is applied in plant growth regulators, microorganism-based methods, and chemicals for biological control, etc. question

Active Publication Date: 2009-03-11
中农绿康(北京)生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term application of chemical pesticides in large quantities not only seriously pollutes people's living environment, destroys ecological balance, threatens the health and safety of humans and animals, but also leads to the emergence of drug resistance of pathogens, which makes diseases rampant again and is more difficult to control.
According to reports, in recent years, some agents of the two main pesticides, benzimidazoles and dicarboximides, have caused the resistance of Botrytis cinerea, resulting in a significant decline in the control effect in the field.

Method used

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  • One strain of streptonmyces lydicus and its application in treatment and prevention of plant's disease
  • One strain of streptonmyces lydicus and its application in treatment and prevention of plant's disease
  • One strain of streptonmyces lydicus and its application in treatment and prevention of plant's disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Streptomyces lydicus A02 CGMCC No. 1654 strain culture and preparation of biocontrol formulations against plant fungal diseases

[0047] Inoculate Streptomyces lydicus A02 CGMCC No.1654 on the above-mentioned Gao's No. 1 slant medium, cultivate at 28°C for 7-10 days, until it produces sufficient spores, scrape it with a sterile platinum ring. 2-3 rings of spores were inoculated into 50ml of the above-mentioned seed medium in a 250ml conical flask, placed on a temperature-controlled shaker, and incubated at 28°C with constant temperature shaking at 200rpm / min (rotation radius 13mm) for 24h-30h; Under bacterial conditions, it was tapped in the above-mentioned fermentation medium in 10 500ml conical flasks (the amount of liquid in each bottle was 100ml); the shake flask after the inoculation was at 31°C under the condition of 240rpm / min (rotation radius 13mm). The rotation speed was shaken for 96h; at this time, the strain had produced a high concentration of an...

Embodiment 2

[0049] Example 2. Antibacterial activity test of Streptomyces lydicus A02 CGMCC No. 1654 and its metabolites

[0050] In the present embodiment, the tested culture medium includes: Gao's No. 1 culture medium, PDA culture medium, seed culture medium and fermentation culture medium, all of which are composed as described above; the tested target pathogenic bacteria is Botrytis cinereaPers .) three strains, namely cucumber botrytis cinerea, tomato botrytis cinerea and bell pepper botrytis cinerea.

[0051] 1. The antibacterial activity test of the plate colonies adopts the plate confrontation culture method. First, the spores of Streptomyces lydicus A02 CGMCC No.1654 on the solid slope are made into a bacterial suspension with sterile water, and 200-500 μl of bacteria are taken. The suspension was evenly spread on the Gao's No. 1 solid plate with a diameter of 90 mm, and cultured at 28 °C for 2-4 days to make it cover the plate; use a sterile hole punch with a diameter of 7 mm to...

Embodiment 3

[0066] Example 3. Greenhouse disease prevention experiment of Streptomyces lydicus A02 CGMCC No.1654

[0067] Tested target pathogens: Botrytis cinerea tomato and Botrytis cinerea

[0068] Test crop varieties; Zhongshu No. 6 tomato, Qimen sweet pepper

[0069] Test control agent: 50% Huikang suspending agent (Pesticide Registration No. Ls20041062, Inner Mongolia Qingyuanbao Biotechnology Co., Ltd.)

[0070] The sterile fermentation filtrate of Streptomyces lydicus A02 CGMCC No. 1654 was prepared according to the method of Example 1. Tomato Botrytis cinerea and sweet pepper Botrytis cinerea were cultured on a PDA plate at 22°C for 7-10 days. After a large number of conidia were grown, the spores were washed with sterile water and prepared into 30 per field of view under a 10×10 magnification microscope. -The bacterial suspension of 50 spores is ready for use.

[0071] Four treatments were set up in the experiment: 5-fold dilution and 2-fold dilution of sterile fermentation f...

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Abstract

This invention discloses the application of Streptomyces lydicus A02 CGMCC No.1654. After fermentation, the strain can produce active metabolite having strong antiseptic effects. The metabolite can be used for manufacturing biological agents for resisting plant fungi.

Description

technical field [0001] The invention relates to a strain of Streptomyces lidi and its application, in particular to the strain of Streptomyces lidi and its application in the biological control of plant diseases. Background technique [0002] Plant diseases caused by fungi account for more than 80% of all plant diseases, and many major diseases cause heavy losses to agricultural production. disease, wheat scab, etc. Among them, Botrytis cinerea caused by Botrytis cinerea is a worldwide epidemic and important disease that commonly occurs in vegetable protected areas. The main diseases or primary diseases of fruits and vegetables such as zucchini, sweet peppers, peppers, kidney beans, leeks, strawberries, grapes, etc., often cause fruit rot and serious losses. In Europe and the United States, Australia, Africa, Japan, India and other countries, the field yield loss caused by gray mold is generally 20-30%. In the areas where the greenhouses in the northeast and northwest of H...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P1/00C12R1/465
Inventor 刘伟成裘季燕刘建华田兆丰刘霆刘德文潘争艳卢彩鸽
Owner 中农绿康(北京)生物技术有限公司
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