Biological fungi-proofing coniothyrium minitans ZS-1SB for preventing and treating sclerotinia as well as preparation method and application thereof

A technology of ZS-1SB, Concetella Shields, applied in biochemical equipment and methods, botanical equipment and methods, microorganism-based methods, etc., to achieve the effects of simple equipment, good permeability, and extensive development value

Inactive Publication Date: 2009-12-16
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because sclerotinia occurs frequently and is popular in the middle and late stages of rapeseed growth, specific pesticide dosages and labor intensity are required to allow pesticides to reach the d...

Method used

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  • Biological fungi-proofing coniothyrium minitans ZS-1SB for preventing and treating sclerotinia as well as preparation method and application thereof
  • Biological fungi-proofing coniothyrium minitans ZS-1SB for preventing and treating sclerotinia as well as preparation method and application thereof
  • Biological fungi-proofing coniothyrium minitans ZS-1SB for preventing and treating sclerotinia as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Isolation and screening of C. shield ZS-1SB strain

[0023] Isolation and Identification: From the soil of the vegetable plantation in Zhushan County, Hubei Province, China, we isolated and screened a strain of M. shieldum that has a strong parasitic effect on Sclerotinia sclerotia. The applicant named it Coniothyrium minitans ) ZS-1SB, the test shows that the isolated ZS-1SB bacterial strain of the present invention has stronger antagonism to the mycelia growth of sclerotinia (S.sclerotium) and the main pathogenic bacteria (Xanthomonas oryzae pv.oryzae) of rice, and can be used as rape Candidate strains of microbial inoculants for the control of Sclerotinia.

[0024] Shell mold ZS-1SB of the present invention separates and screens and adopts sclerotinia sclerotia trapping method (Adames etc., 1980) and dilution plate method, strain identification work with reference to Wei Jingchao, "Handbook of Fungus Identification" Shanghai Science and Technology Publishing House...

Embodiment 2

[0032] Embodiment 2: the semi-open solid fermentation culture of shell mold ZS-1SB bacterial agent

[0033] 1. Preparation of C. shield ZS-1SB spore suspension: place C. shield ZS-1SB strain on the slant of PDA medium (see the same formula) and culture at 20° C. for 7 days. Rinse the slope with sterile water and adjust the conidia suspension to 10 6 Spores / ml.

[0034] 2, the multiplication culture of shell mold ZS-1SB: the conidia suspension of shell shell mold ZS-1SB of step 1 is 1: 100 inoculation in PDB substratum with mass ratio (prescription: potato 200g, glucose 20g, Supplement distilled water to 1000ml, adjust the pH to 7.0), divide the PDB medium into Erlenmeyer flasks (100ml / 250ml per bottle), shake and cultivate at 150r / min, 20°C for 24h, and obtain Clamella ZS -1SB bacteria liquid, spare.

[0035] 3. Shell mold ZS-1SB inoculum semi-open solid fermentation culture: dry compost (water content generally no more than 8%) oatmeal (commercially purchased) and rice bra...

Embodiment 3

[0036] Embodiment 3: the activity test of shell fungus ZS-1SB bacterial strain parasitic, cause Sclerotinia saprophytum sclerotia

[0037] 1. Sclerotinia sclerotium and preparation: The reported Sclerotinia sclerotium strain (Sclerotinia sclerotium) Ep-1PNA367 (Huiquan Liu, Yanping Fu, Daohong Jiang, Guoqing Li, Jun Xie, Youliang Peng, Xianhong Yi, and Said A. Ghabrial.A Novel Mycovirus That Is Related to the Human Pathogen Hepatitis E Virus and Rubi-Like Viruses.JOURNAL OF VIROLOGY, Feb.2009, 1981-1991) as a control, Sclerotinia strain Ep-1PNA367 was inoculated in carrot medium ( Carrots were cut into strips, sterilized at 121°C for 30min), and cultured at 20°C for 60d to obtain mature sclerotia.

[0038] 2, the preparation of shell mold ZS-1SB: get in the embodiment 2 in the fermented culture terminal stage of ZS-1SB the culture culture containing bacterial agent appropriate, add tap water and wash by rubbing with the hands repeatedly, wash out the conidia liquid of shell sh...

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Abstract

The invention belongs to the technical field of biological pesticide, in particular to screening and identification of a coniothyrium minitans strain with strong parasitism and saprogenicity on sclerotinia sclerotium and an application thereof in a microorganism agent. The coniothyrium minitans ZS-1SB strain is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number being CCTCC NO: M209114. The invention also discloses an open-type solid fermentation cultivation method of the microorganism agent and an application of the coniothyrium minitans ZS-1SB microbial inoculum in the prevention and treatment of sclerotinia of colza. The microorganism agent of the invention has excellent biological prevention effect on plant fungous diseases such as sclerotinia rot of colza and the like.

Description

technical field [0001] The invention relates to the technical field of microbial pesticides, in particular to a Clamella shield strain with biocontrol potential for Sclerotinia sclerotiorum and a semi-open solid fermentation culture method and application thereof. Background technique [0002] Sclerotinia sclerotiorum (Lib.) de Bary can infect more than 540 kinds of plants in 64 families, including oilseed rape, soybean, sunflower and other important vegetable crops such as lettuce, Chinese cabbage, celery and carrot. Causes sclerotinia in a variety of plants worldwide. Since no planting resources with obvious disease resistance have been found so far, and no disease-resistant varieties can be used, the sclerotinia caused by it is still a worldwide problem in agricultural production. At present, chemical pesticides are mostly used for control. For example, spraying chemical pesticides "Sclerotinia" or "Carbendazim" during the flowering stage of rapeseed can alleviate the ha...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 姜道宏李国庆付艳苹程家森谢甲涛
Owner HUAZHONG AGRI UNIV
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