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A kind of Paecilomyces lilacinus and its cultivation method and application

A technology of Paecilomyces lilacinus and a culturing method is applied to the application of Paecilomyces lilacinus in medicines for preventing and treating cyst nematode disease of graminearum, and the cultivation field of Paecilomyces lilacinus can solve the problem of low antibacterial control effect, Restricted development and utilization, low parasitic rate of strains, etc., to achieve obvious lethal effect, extensive development value, and simple equipment

Active Publication Date: 2015-08-19
JIANGXI NEW DRAGON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the parasitic rate of the strains screened naturally is not high, the development effect of the dosage form is not good, the biocontrol mechanism is not clear, and the control effect of the bacterial agent is low, which limits the development and utilization of the bacteria

Method used

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  • A kind of Paecilomyces lilacinus and its cultivation method and application
  • A kind of Paecilomyces lilacinus and its cultivation method and application
  • A kind of Paecilomyces lilacinus and its cultivation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A liquid-solid two-phase fermentation culture method for Paecilomyces lilacinus (P.lilacinus)

[0036] The steps are:

[0037] 1. Acquisition of Paecilomyces lilacinus PL461 strain

[0038] The wild-type Paecilomyces lilacinus 36-1 strain was used as the starting strain, which was reported by Fu Yanping and Wang Mingzu in "Journal of Huazhong Agricultural University" in 1998. The strain obtained a mutant strain PL461 with stable drug resistance and large spore production through ultraviolet mutagenesis, and was continuously subcultured for 10 generations for tolerance and domestication. It was the target strain, and the applicant named it Paecilomyces lilacinus (P. lilacinus) PL461. Experiments show that the mutant strain PL461 has a parasitic rate as high as 61.01% on the 10th day after inoculating the embryonic eggs and mixed eggs containing larvae of cereal cyst nematode, and can be used as a candidate strain for the control and microbial agent of cereal cyst nemat...

Embodiment 2

[0048] Embodiment 2: Paecilomyces lilacinus PL461 strain is parasitic and lethal to cereal cyst nematode eggs and cysts

[0049] 1. Preparation of test and nematodes:

[0050] In this embodiment, Paecilomyces lilacinus PL461 strain screened by the applicant was used as the test strain. Cereal cyst nematode cysts were isolated from wheat diseased soil samples collected in Xiangyang, Hubei, and nematode eggs were collected. The surface was disinfected and used as test nematodes.

[0051] Activation conditions of Paecilomyces lilacinus PL461 bacterial strain: 26~28 DEG C on PDA medium (recipe reference example 1) plate, after cultivating for 5~9 days, the spore concentration is prepared to be 7×10 9 Spore fluid.

[0052] Collection and separation of cereal cyst nematode ova and cysts: adopt floating drum separation method (referring to Kim D G, separation method in 1994) to separate cysts from wheat diseased soil in Xiangyang, Hubei, and adopt 35% sucrose centrifugation Collec...

Embodiment 3

[0057] Embodiment 3: Utilize Paecilomyces lilacinus PL461 bacterial agent to control the field test of cereal cyst nematode disease

[0058] In order to verify the disease prevention effect of the Paecilomyces lilacinus PL461 inoculum produced by the present invention, the applicant selected a serious and uniform plot of wheat in Niushou Town, Xiangyang City, Hubei Province, according to the cultivation system, cultivar, soil texture, fertilizer and water conditions and Meteorological conditions, it is divided into 18 sub-districts, each sub-district is 10m2 , field control experiment of cereal cyst nematode was carried out.

[0059] 1. Test drug:

[0060] Paecilomyces lilacinus PL461 inoculum: get the appropriate amount of culture containing Paecilomyces lilacinus PL461 inoculum PLC in the final stage of fermentation in Example 1, and the usage amount is divided into four kinds of treatments: 25kg / ha, 50kg / ha, 75kg / ha ha and 100kg / ha; (ha is the area unit: hectare)

[0061]...

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Abstract

The invention discloses paecilomyces lilacinus and a cultivating method thereof and applications thereof. The method comprises steps of: A. activation of paecilomyces lilacinus PL461, which comprises steps of: placing paecilomyces lilacinus PL461 bacterial strains on a PDA medium board, cultivating the paecilomyces lilacinus PL461 bacterial strains, and activating the paecilomyces lilacinus PL461 bacterial strains; B. liquid fermentation cultivation of the paecilomyces lilacinus PL461, which comprises steps of: putting the activated paecilomyces lilacinus PL461 bacterial strains into an Erlenmeyer flask containing a liquid medium of PDB and Czapek's, cultivating the above bacterial strains with shaking, and taking the above cultivated bacterial strains as standby; C. solid fermentation cultivation of the paecilomyces lilacinus PL461, which comprises steps of: mixing a solid fermentation cultivation medium uniformly according to a volume ratio, wherein the medium comprises dry compost rice bran, corn flour and water, putting the mixed medium into a domestic fungus culture bag, inoculating the paecilomyces lilacinus PL461 fermented bacteria solution into the solid fermentation cultivation medium employing culture dish fermentation after disinfection and cooling, and putting the above inoculated solid fermentation cultivation medium into a culture dish after stirring uniformly again to cultivate and take as standby; and D. preparation of a paecilomyces lilacinus bacteria agent from the solid phase culture according to a formula and the method. The paecilomyces lilacinus has an obvious lethal effect on nematode eggs, equipment is simple, production cost is reduced greatly, and the cultivating method is suitable for mass production of paecilomyces lilacinus.

Description

technical field [0001] The invention relates to the technical field of microbial pesticides, in particular to Paecilomyces lilacinus, a method for cultivating Paecilomyces lilacinus, and a drug for preventing and treating cereal cyst nematodes by Paecilomyces lilacinus in the application. Background technique [0002] Cereal Cyst Nematode (CCN) caused by wheat cereal cyst nematode (Heterodera avenae), which mainly infects dryland grasses, has been widely distributed in five continents since it was first discovered in Germany in 1874. More than 40 wheat-producing countries have occurred and caused damage, and the area of ​​wheat damaged by cereal cyst nematodes in my country has exceeded 3 million hectares. Grain cyst nematode is still a worldwide problem in agricultural production because no germplasm resources with obvious disease resistance have been found so far. At present, chemical nematocides are often used to prevent and control the disease, such as 35% carbofuran se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/79
Inventor 肖炎农张春龙成儒萍向妮李田田杨凡
Owner JIANGXI NEW DRAGON BIOTECH
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