The invention discloses a
caffeine extract of goat grass vulgaris and its extraction method and application, belonging to the field of bioengineering. The specific steps of the extraction method of the
caffeine extract of Goatweed vulgaris in the present invention are as follows: take the root of Goatweed vulgaris, wash it,
cut it into slices, dry it in an oven at 80°C for 6 hours to remove
moisture thoroughly, and then pulverize it with a
pulverizer , after passing through a 60-mesh
sieve, put 10 grams of pulverized goat grass root samples into a 50-milliliter extraction kettle, and use absolute
ethanol as an entrainer. 2 30MPa in the extractor, extract at 60°C for 2h, in which CO 2 The flow rate is controlled at 1.5LPM to obtain the
caffeine extract of mutable goat grass. The research of the present invention shows that >0.1% caffeine has a
growth inhibitory effect on
plant pathogenic nematodes, and the caffeine extract of goat grass vulgaris obtained in the present invention can be used to prepare
insect-resistant insecticides for cereal
cyst nematodes and root-knot nematodes incognita agent.