36 results about "Cereal cyst nematode" patented technology

The invention discloses a lysine bacillus for preventing and controlling plant nematodes and an application thereof. The name of Lysinibacillus sp. provided by the present invention is BDGS‑17‑07, and the preservation number is CGMCC No.15617. Experiments show that BDGS‑17‑07 fermented liquid has poisonous activity to the second instar larvae of graminearum cyst nematode and the eggs and second instar larvae of root-knot nematode incognita, and also has poisonous activity against egg grains and egg mass hatching of root-knot nematode incognita. The BDGS‑17‑07 fermentation broth also has a good biocontrol effect. Only 50mL of the BDGS‑17‑07 fermentation broth is applied, and the control effect can reach 36% when inoculated with nematodes for 60 days. The Bacillus lysinica BDGS‑17‑07 CGMCC No.15617 of the present invention has good application and development prospects as a biological agent in the prevention and treatment of plant parasitic nematodes.

The invention discloses a Microdochium bolleyi F10 strain microbial inoculum as well as a preparation method and application thereof. The preparation method comprises the following steps: (1) activating a Microdochium bolleyi F10 strain on a PDA (potato dextrose agar) flat plate, then inoculating the strain to a PDA inclined plane for culture, adding sterilized deionized water to prepare a spore suspension, adding tween-20 to ensure that the final concentration of the tween-20 in the spore suspension is 0.025%, and adjusting to ensure that the concentration of the spore suspension reaches 1.0*10<7> / ml; (2) uniformly mixing dry culture material oatmeal with rice husks, adding water, and performing high-pressure steam sterilization; and (3) inoculating the prepared spore suspension to the culture material according to a ratio of 10ml / bag, culturing, airing and crushing to obtain the microbial inoculum. The Microdochium bolleyi F10 strain prepared by using the preparation method has a strong parasitic effect on cysts of heterodera avenae wollenwebers, the control effect of a bio-control microbial inoculum on cereal cyst nematode under a field cultivation condition reaches 41%, an application method of soil treatment is simple and saves labor force, and a biological pesticide is safe to human and livestock.

Disclosed are a Bacillus pumilus strain and the use thereof in preventing and treating cereal cyst nematode in wheat, which strain is a Bacillus pumilus named B202 and deposited in CGMCC with a deposit number of CGMCC NO. 5838. Also disclosed is the use of the Bacillus pumilus strain B202 in the preparation of a biological protective preparation for cereal cyst nematode in wheat. The active ingredients of the biological protective preparation for cereal cyst nematode in wheat comprise at least one of the following (a) or (b) or (c) or (d): (a) a fermentation culture of the Bacillus pumilus strain B202; (b) the Bacillus pumilus strain B202; (c) spores of the Bacillus pumilus strain B202; and (d) a fermentation supernatant of the Bacillus pumilus strain B202. The Bacillus pumilus strain B202 has a stable prevention and treatment effect on cereal cyst nematode in wheat.

The invention relates to a heterodera avenae sensu lato Ha-63744 protein, an encoding gene and the application of the heterodera avenae sensu lato Ha-63744 protein. A sequence of the heterodera avenae sensu lato Ha-63744 protein is shown as SEQ ID NO: (sequence identifier number) 1, and a gene sequence encoding the protein is shown as SEQ ID NO: 2. The Ha-63744 gene is expressed in an esophageal gland of heterodera avenae sensu lato, and an expression level is higher in third and fourth instar larva periods after parasitism of the heterodera avenae sensu lato. After the silent Ha-63744 gene is treated by dsRNA (double-stranded ribonucleic acid), the quantity of the heterodera avenae sensu lato at a wheat root is obviously less than that of a control, which shows that the gene plays an important role in a parasitism pathogenesis process of the heterodera avenae sensu lato and can serve as a target gene of a heterodera avenae sensu lato resistance project of a plant. The protein and the gene have a great value for pathogenic mechanism research of the heterodera avenae sensu lato and preparation of the heterodera avenae sensu lato resistance plant.

The present invention disclosed a kind of vulnerable goat alanine aminotramerase nucleic acid sequence and its application.The present invention is easy to change the goat phenylacease gene to encodes a phenylalanine, which has a high performance of anti -gray cysts, which has important reference and application value for the breeding of wheat anti -gravida cysts.

The invention relates to a rapid detection method of heterodera avenae wollenweber LAMP and application of the detection method. Five LAMP primers, namely HA5-F3, HA5-B3, HA5-FIP, HA5-BIP and HA5-LB are designed and screened according to a heterodera avenae wollenweber random amplified polymorphic dna (RAPD) sequence obtained by cloning; and an LAMP reaction system is configured and optimized. By performing DNA extraction, loop-mediated isothermal amplification, amplified product developing and observation on the heterodera avenae wollenweber, the heterodera avenae wollenweber can be rapidly detected. The detection method is high in specificity, low in cost and convenient to operate, and has high application values on rapid detection of the heterodera avenae wollenweber and early and field diagnosis of the heterodera avenae wollenweber diseases.

The invention discloses a caffeine extract of goat grass vulgaris and its extraction method and application, belonging to the field of bioengineering. The specific steps of the extraction method of the caffeine extract of Goatweed vulgaris in the present invention are as follows: take the root of Goatweed vulgaris, wash it, cut it into slices, dry it in an oven at 80°C for 6 hours to remove moisture thoroughly, and then pulverize it with a pulverizer , after passing through a 60-mesh sieve, put 10 grams of pulverized goat grass root samples into a 50-milliliter extraction kettle, and use absolute ethanol as an entrainer. 2 30MPa in the extractor, extract at 60°C for 2h, in which CO 2 The flow rate is controlled at 1.5LPM to obtain the caffeine extract of mutable goat grass. The research of the present invention shows that >0.1% caffeine has a growth inhibitory effect on plant pathogenic nematodes, and the caffeine extract of goat grass vulgaris obtained in the present invention can be used to prepare insect-resistant insecticides for cereal cyst nematodes and root-knot nematodes incognita agent.