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Observation and determination method of effect of trichoderma T6 strain on plant parasitic nematode

A technology of plant pathogenicity and determination method, applied in the field of observation and determination of the effect of Trichoderma T6 strain on plant pathogenic nematodes

Inactive Publication Date: 2019-08-02
GANSU AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the effect of the conidia suspension of Trichoderma T6 strain on the microstructure and activity of cysts, eggs and 2nd instar larvae of Cereal cyst nematode

Method used

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  • Observation and determination method of effect of trichoderma T6 strain on plant parasitic nematode
  • Observation and determination method of effect of trichoderma T6 strain on plant parasitic nematode
  • Observation and determination method of effect of trichoderma T6 strain on plant parasitic nematode

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Embodiment 1

[0034] The present invention will be further described in conjunction with embodiment, accompanying drawing:

[0035] 1.1 Microscopic observation method of Trichoderma T6 strain on cyst lethality and parasitism

[0036] Add 5 surface-sterilized and uniformly developed cysts and 1mL concentration of (1.5×10 5 -10 7 cfu mL -1 ) Trichoderma T6 strain spore suspension, to add 1mL Fusarium oxysporum spore suspension (1.5×10 7 cfu mL -1 ) and sterile water were used as controls respectively, and the test included 5 treatments, and each treatment and control had 6 repetitions. The test was carried out at 20°C, and the process of cyst parasitization and death was observed and recorded under a microscope every 2 days.

[0037] 1.2, Trichoderma T6 strain to cyst parasitic and lethal effect assay method

[0038] Add 10 surface-sterilized and more consistent cysts into a high-temperature sterilized petri dish with a diameter of 3.5 cm. Then, add 1mL different concentrations of Tric...

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Abstract

The invention belongs to the technical field of microorganism bio-control prevention, and particularly relates to an observation and determination method of effects of a trichoderma T6 strain on plantparasitic nematodes. The method is mainly characterized by comprising the following steps of adding sporocysts, second-age larvae and ova into a culture vessel; or adding second-age larvae, ova and trichoderma T6 strain spore suspension into a sterilized and disinfected 90-hole cell culture plate; adding fusarium oxysporum spore suspension and sterile water as reference respectively; performing microscopic observation and determination on the sporocyst parasitism and hatching, second-age larva death and ovum parasitism or hatching conditions. The method has the beneficial effects that throughmicroscopic observation and determination, the parasitism and lethal action mechanism of the trichoderma T6 strain on cereal cyst nematodes is analyzed and discussed, so that the theoretical basis isprovided for the preparation, development and reasonable application of novel and efficient microbial nematode killing agents.

Description

technical field [0001] The invention belongs to the technical field of microbial biocontrol, and in particular relates to a method for observing and measuring the effect of Trichoderma T6 strain on plant pathogenic nematodes. Background technique [0002] In the production of crops, the control of plant parasitic nematodes is still dominated by chemical control, mainly through the use of chemical nematicides to control their infection and damage. The nematicides used in the early days, such as Carbofuran, Etho-prophos and Miral, have a control effect of up to 90% against nematodes, but due to their high residue and high toxicity, and the Beneficial microorganisms are killed and other defects. At the same time, the application of nematicides has also brought about a series of problems such as toxicity to humans and animals, difficulty in degrading in soil, and environmental pollution. In addition, some synthetic nematicides have non-specific toxicity to target nematodes, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033C12Q1/02
CPCA01K67/033C12Q1/02
Inventor 张树武徐秉良刘佳李通
Owner GANSU AGRI UNIV
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