Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Oxalic acid penicillium NBC008 with nematocidal activity, and preparation method and application thereof

A nematicidal and active technology is applied in the field of fungi capable of killing plant parasitic nematodes and the preparation thereof, and can solve the problems of less nematodes and the like

Inactive Publication Date: 2016-11-09
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, few biocontrol agents have been developed to control nematodes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oxalic acid penicillium NBC008 with nematocidal activity, and preparation method and application thereof
  • Oxalic acid penicillium NBC008 with nematocidal activity, and preparation method and application thereof
  • Oxalic acid penicillium NBC008 with nematocidal activity, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Preparation of fermentation broth of bacterial strain P.oxalicum NBC008

[0034]Inoculate the P. oxalicum NBC008 mycelia on a PDA plate, and cultivate it at 26°C for 10 days. Take a bacterium cake with a diameter of 1 cm from the plate and inoculate it into a 250ml Erlenmeyer flask (with 100ml PDB inside), 26 Cultivate with shaking at 150rpm / min in the dark for 120h. Use 4 layers of sterilized gauze to remove the mycelia and pass through a 0.22 μm bacterial filter to sterilize to obtain the fermentation stock solution. The fermentation stock solution was diluted in different times to determine the nematicidal efficacy.

Embodiment 2

[0035] Example 2: Nematicidal activity of strain P. oxalicum NBC008

[0036] On the basis of the stock solution of the fermentation broth, it was diluted 1×, 5× and 10× sequentially, and the 2nd instar larvae of soybean cyst nematode were tested.

[0037] 1 Preparation of test agents

[0038] The fermented liquid of the fungal strain P.oxalicum NBC008 prepared according to the aforementioned liquid fermentation method was used to determine the nematicidal activity of the fermented liquid with different dilution ratios.

[0039] 2 Preparation of nematodes for experiment

[0040] 2.1 Collection of soybean cyst nematode cysts

[0041] Pour the diseased soil harmed by soybean cyst nematodes collected from the Langfang pilot test base of the Academy of Agricultural Sciences into a clean plastic small bucket, wash away the diseased soil with strong water, pass through a 20 mesh / 80 mesh sieve, and collect 80 mesh Sediments on the mesh screen, cysts were picked under a dissecting m...

Embodiment 3

[0067] Example 3: Determination of indoor control effect of bacterial strain P.oxalicum NBC008 fermentation broth

[0068] Using fermented stock solution to control soybean cyst nematode and wheat cyst nematode, the effect is as follows:

[0069] 1 Test materials:

[0070] Soybean variety: Zhonghuang 13

[0071] Nematode: soybean cyst nematode

[0072] 2 Test method:

[0073] Determination of the effect of fermentation broth of strain P.oxalicum NBC008 on indoor control of soybean cyst nematode

[0074] The surface of soybean seeds was sterilized with 1% sodium hypochlorite, washed three times with sterilized water, and placed in a disposable petri dish to germinate. After 2 days the germinated seeds were planted in plastic cups filled with sterilized soil, 3 seeds per cup. One week later, 20 ml of the fungal fermentation stock solution was added to the plastic cup, and PDB was used as a control. After 24 hours of fermentation, inoculate 300 SCN second-instar larvae near...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to oxalic acid penicillium NBC008 with nematocidal activity, and a preparation method and application thereof. A fungus strain P.oxalicum NBC008 is separated and screened from a cereal cyst nematode colony in Ostfold county of Norway, and the collection number is CGMCC No. 12473. Through liquid fermentation and cultivation, fermented products have obvious toxic action on plant parasitic nematodes. Influences of strain fermentation liquid on second-instar larvas of soybean cyst nematodes and egg incubation are measured indoors. The fermentation liquid with different dilution concentrations has toxic action on the second-instar larvas of the soybean cyst nematodes, and has obvious inhibition effect on egg incubation. Fermentation stock solution can reduce the number of soybean sporocysts by 59.88%, and has good development and application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, in particular to a fungus capable of poisoning and killing plant parasitic nematodes and its preparation method and application. Background technique [0002] Plant parasitic nematodes are one of the important plant pathogens, which generally occur all over the world, have a wide range of hosts, and cause serious damage. The annual economic losses caused by plant parasitic nematodes in the world are as high as 157 billion U.S. dollars (Jian Heng, 2011. Plant Nematology . Beijing: China Agricultural University Press). Among them, soybean cyst nematode (Soybean Cyst Nematode, SCN) is one of the most serious plant parasitic nematodes. [0003] Soybean cyst nematode was first discovered in my country (1899), and its original scientific name was Heterodera schachtii, which is the scientific name of sugar beet cyst nematode. In 1952, through careful observation, Ichinohe independently es...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/04A01P5/00C12R1/80
CPCA01N63/30C12N1/145C12R2001/80
Inventor 彭德良李婷黄文坤孔令安彭焕崔江宽
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com