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A strain of Bacillus lysinus for preventing and controlling plant nematodes and its application

A Bacillus lysine, plant technology, applied in the application, nematicides, plant growth regulators and other directions, can solve serious pollution, damage to the environment, high residues and other problems

Active Publication Date: 2021-08-27
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the control of plant parasitic nematodes is still dominated by chemical control, but there are few varieties of chemical nematicides, and most of them are highly toxic and high residue, which seriously pollute and damage the environment

Method used

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  • A strain of Bacillus lysinus for preventing and controlling plant nematodes and its application
  • A strain of Bacillus lysinus for preventing and controlling plant nematodes and its application
  • A strain of Bacillus lysinus for preventing and controlling plant nematodes and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1, Isolation, Purification and Identification of BDGS-17-07 Bacterial Strain

[0044] 1. Isolation and purification of BDGS-17-07 strain

[0045] Strain BDGS-17-07 was isolated from the soil of Bagagong Mountain in Zhangjiajie, Hunan Province. The specific steps are as follows: Weigh 5g of soil sample, add it into a conical flask filled with 45mL of sterile water, shake and cultivate the conical flask for 30min in a shaker with a rotating speed of 200rpm / min, and prepare 10 -1 soil dilution. Step-by-step dilutions were carried out in an ultra-clean workbench to prepare a series of soil dilutions with corresponding gradients. Use a pipette gun to draw an appropriate amount of soil dilution on the LB plate, and then spread it evenly with a spreader, and repeat each concentration 3 times. Place it in a constant temperature incubator at 28°C for 24 hours, observe the growth of the colony on the plate, pick a single colony on the plate, name it BDGS-17-07 strain, ...

Embodiment 2

[0054] The preparation of embodiment 2, BDGS-17-07 bacterial agent

[0055] 1. Culture of plate strains

[0056] The BDGS-17-07 strain isolated in Example 1 was inoculated on an LB solid medium plate, cultured at 28°C for 24 hours, and a single colony was picked to obtain a plate strain.

[0057] 2. Liquid fermentation culture

[0058] Inoculate the plate bacteria obtained in step 1 into a 250mL Erlenmeyer flask containing 100mL of fermentation medium, and ferment and culture at 200r / min shaker speed for 60h at 28°C to obtain BDGS-17-07 and its metabolites. The fermented liquid is named BDGS-17-07 bacterial agent.

Embodiment 3

[0059] Embodiment 3, the application of BDGS-17-07 bacterial agent in the prevention and treatment of plant parasitic nematodes

[0060] 1. Nematicidal activity test of BDGS-17-07 bacterial agent on second instar larvae of plant parasitic nematodes

[0061] 1. Obtaining the second instar larvae of the root-knot nematode Meloidogyne incognita

[0062] Take water spinach roots inoculated with second instar larvae of Meloidogyne incognita for 60-90 days, clean the root soil with tap water, pick mature and plump egg masses with tweezers under a magnifying glass and place them in the hatching sieve, and place the hatching sieve containing a large number of egg masses Place in a petri dish, add distilled water to the petri dish until the eggs are slightly submerged, and hatch in a constant temperature incubator at 25°C. In order to ensure the complete rate of hatched nematodes, the second instar larvae hatched on the first day are discarded; The activity of the second-instar larvae...

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Abstract

The invention discloses a lysine bacillus for preventing and controlling plant nematodes and an application thereof. The name of Lysinibacillus sp. provided by the present invention is BDGS‑17‑07, and the preservation number is CGMCC No.15617. Experiments show that BDGS‑17‑07 fermented liquid has poisonous activity to the second instar larvae of graminearum cyst nematode and the eggs and second instar larvae of root-knot nematode incognita, and also has poisonous activity against egg grains and egg mass hatching of root-knot nematode incognita. The BDGS‑17‑07 fermentation broth also has a good biocontrol effect. Only 50mL of the BDGS‑17‑07 fermentation broth is applied, and the control effect can reach 36% when inoculated with nematodes for 60 days. The Bacillus lysinica BDGS‑17‑07 CGMCC No.15617 of the present invention has good application and development prospects as a biological agent in the prevention and treatment of plant parasitic nematodes.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a lysine bacillus for preventing and controlling plant nematodes and its application. Background technique [0002] Among the more than 25,000 known species of nematodes, there are more than 4,100 species of plant parasitic nematodes, accounting for about 15% of the total number of known nematodes, and the annual losses to the world's agricultural production are estimated to reach 157 billion US dollars. There are many types of plant-parasitic nematodes, mainly including Meloidogyne, Anguina, Ditylenchus, Heterodera, Aphelenchoides and root nematodes. Ectoparasitic nematodes, etc. Among them, root-knot nematodes and cyst nematodes are two types of plant parasitic nematodes that cause serious damage in agricultural production, and their disease on vegetables and food crops will cause relatively significant economic losses. [0003] At present, the control of plant parasitic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P5/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 刘倩简恒王新新
Owner CHINA AGRI UNIV
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