112 results about "Dauer larva" patented technology

The invention relates to a culture method of a fungus capable of poisoning plant parasitic nematodes, and a preparation method of a metabolite thereof and application thereof, and belongs to the technical field of microbial pesticides. A fungus strain (Aspergillus niger Y-61) related in the invention was collected in China General Microbiological Culture Collection Center on August 7, 2008, and the collection number is CGMCC No.2631. The taxonomic status of the Y-61 is a mitosporic fungus, hyphomycetes, moniliales, moniliaceae, aspergillus and aspergillus niger. The fungus metabolite is prepared by liquid fermentation and culture, has outstanding characteristics of high toxicity to the plant parasitic nematodes, especially root-knot nematodes, and has obvious nematodes killing effect; and the influence of the strain fermentation broth on Meloidogyne incognita juveniles (J2) and oocyst hatching is determined indoors. Treatment of the fermentation broth with different diluted concentrations is significantly different from the aseptic water treatment, and the preventive effect of the fermentation broth with less than one-fifth concentration is be equivalent to that of 10mug/ml of cadusafos. The fungus has good application and development prospects.

The invention relates to a culturing method of streptomyces with the function of poisoning plant menatodes and a preparation method and application of the metabolin, belonging to the technical field of microbial pesticides. The streptomyces bacterial strain Snea253 is Streptomyces venezuelae, stored in China General Microbiological Culture Collection Center on January 18, 2008 (address: Datun Road, Zhaoyang District, Beijing; post code: 100101) with CGMCC No.2348. The streptomyces bacterial strain Snea253 is classified in species Streptomyces venezuelae, genus streptomyces, order actinomycetales, class actinomycetales, phylum actinomycetales. The culturing method of streptomyces with the function of poisoning plant menatode has the advantages that: through liquid fermentation culture preparation, the metabolites can poison plant parasitic nematodes, in particular Heterodera glycines, Meloidogyne spp. and Aphelenchoides besseyi with distinct effects; the fermentation liquid of the bacterial strain has higher poisonous activity to second instar larva of Heterodera glycines, and can remarkably restrain cyst hatching; the treatment of fermentation liquid with different diluted concentration is significantly different from the treatment of sterile water; the insecticidal effects of stock fermentation solution can reach above 90%, and the effects of greenhouse experiment are obvious; at the same time, the bacterial strain can remarkably poison the Meloidogyne spp. and Aphelenchoides besseyi with good application and development prospects.

The invention discloses a method for assisting in identifying root-knot nematodes and a special primer pair thereof. The invention provides a special primer consisting of a primer pair A and a primer pair B, wherein the primer pair A consists of DNA (Deoxyribose Nucleic Acid) shown as a sequence 1 in a sequence table and DNA shown as a sequence 2 in the sequence table; the primer pair B consists of DNA shown as a sequence 3 in the sequence table and DNA shown as a sequence 4 in the sequence table; and the special primer can be used for assisting in identifying the root-knot nematodes. The method provided by the invention can be used for correctly distinguishing four common root-knot nematodes, has high sensitivity, and can be used for identifying the types of root-knot nematodes by using a single egg capsule or a single second stage juvenile. The root-knot nematode can be directly separated from diseased tissues without purification culturing, the purification culturing time of the nematodes can be greatly shortened, and the detection efficiency can be improved.

The invention discloses a lecanicillium psalliotae strain with the preservation number of CGMCC NO: 5329. The strain can colonise spawns, two-year-old larvae and female adults at different life stages of rootknot nematode. Activations of chitinous substance degradative enzyme and excisionenzyme generated by the strain can be respectively determined through an N-acetyl glucosamine method and a p-nitrobenzene method, the enzymatic activities thereof can reach 3.98 U/h.mL and 0.38 U/h.mL, and higher activity of the produced chitinous substance enzyme can be detected at different temperature (27 DEG C-75 DEG C) and different pH (3.0-7.0); and the influence of chitinous substance enzyme extract produced by the strain on eclosion of rootknot nematode spawns is determined in vitro, and the inhibition ratio and the destruction ratio of enzyme solution on relative eclosion of the spawns are respectively 94.52 percent and 84.32 percent. The lecanicillium psalliotae strain disclosed by the invention is applied to biological control of crop rootknot nematodiasis, provides an effective way for protecting sweet potatoes, fruits, vegetables in north China and particularly controlling destructiverootknot nematodiasis, and has obvious ecological benefit, economic value and social value.

The invention discloses a plant insecticide for preventing and treating vegetable root nematodes and a preparation method thereof. The plant insecticide is prepared from the following raw materials in parts by weight: 20-30 parts of garlic juice, 15-25 parts of cortex granati, 15-25 parts of fructus cnidii, 10-20 parts of picrorhizae, 5-10 parts of onion juice, 2-5 parts of melia azedarach leaves and 2-5 parts of polygonum hydropiper. The plant insecticide adopts a plurality of types of natural compound active substances, has insect killing, insect repelling, larva growth and development inhibiting, sterilizing and antibacterial effects, and also has the effect of preventing diseases and disinfecting. The plant insecticide has good insect killing and insect repelling effects and strong selectivity, is safe and stable for people and livestock, has no pollution to the environment, and has no damages to crops.

The present invention provides a strain of Ochrobactrum pseudogrignonense NC1 and applications of the biocontrol microbial agent of Ochrobactrum pseudogrignonense NC1. According to the present invention, the results of the biological test of Ochrobactrum pseudogrignonense NC1 fermentation broth on the egg and the second instar larvae of Melanogyne incognita show that the strain can inhibit the Melanogyne incognita egg hatching and the second instar larvae activity through the volatile nematode killing active substance in the fermentation broth; after the biocontrol microbial agent prepared by using the Ochrobactrum pseudogrignonense NC1 as the active component is applied on potted tomatoes, the test results show that the strain can significantly increase the tomato above-ground part fresh weight, achieves the Melanogyne incognita prevention effect of 62.5%, and has the effect equivalent to the nematode killing agent 10% fosthiazate; and the Ochrobactrum pseudogrignonense NC1 and the biocontrol microbial agent thereof provide good application prospects in prevention and control of root-knot nematode in vegetables.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) primer group and method for rapidly detecting ditylenchus destructor from complex samples. The LAMP primer group comprises a pair of outer primers F3/B3, a pair of inner primers FIP/BIP and a loop primer LF, and sequences of the primers are sequentially shown as SEQ ID NO. 1-5. The LAMP primer group and the detection method thereof disclosed by the invention have excellent specificity and sensitivity for detecting the ditylenchus destructor, individuals of the ditylenchus destructor in different insect stages (such as eggs, larva, female and male) can be detected, the ditylenchus destructor can be directly detected from multiple nematode mixed samples, plant tissue samples and soil samples, and the detection sensitivity can reach 1/1000 individual DNA. A novel detection method is provided for rapid detection and identification and early diagnosis of the ditylenchus destructor, and the method has the advantages ofbeing accurate, sensitive, stable and intuitive in result judgment. The LAMP primer group disclosed by the invention is simple and convenient to operate and high in practicality, and has important significances and application value on quarantine on ports and transfer and origin monitoring operations.

The invention relates to a method for verifying anti-chalk disease traits of a swarm by an SNP marker. The method comprises the following steps: collecting larva samples from Italian swarms to be observed, extracting larva DNA, synthesizing a target primer, carrying out PCR with each extracted individual DAN as a template, carrying out electrophoresis detection on a PCR product, sequencing, finally comparing and analyzing the obtained sequencing result, and verifying the anti-chalk disease traits by judging whether the occurrence frequency PC of a C allele of randomly captured larva individuals from the swarm has difference with the occurrence frequency PT of a T allele in the SNP marker C2587245T. According to the invention, aiming at the problem that the Italian swarms are severely affected by the chalk disease, the SNP (C2587245T) marker related to anti-chalk disease of Italian bee larva provided from the molecular biology level for scientifically, accurately and quickly verifying the strength of the anti-chalk disease performance of the Italian swarms, the breeding cycle of the anti-chalk disease breeding of the Italian bees can be greatly shortened, the bee breeding speed is accelerated and the loss in the beekeeping industry caused by the chalk disease is reduced.

The invention discloses a soybean cyst nematode Hg-flp-1 gene as well as an encoding protein and application thereof, and relates to a soybean cyst nematode neuropeptide gene as well as an encoding protein and application thereof. The invention aims to solve the problem of environmental pollution in the existing chemical control method of soybean cyst nematode. The nucleotide sequence of the geneis shown as SEQ ID NO: 1 in a sequence table. The amino acid sequence of the encoded protein is shown as SEQ ID NO: 2 in a sequence table. After the neuropeptide gene Hg-flp-1 is subjected to dsRNA treatment, the number of second-stage instar larvae in infected roots and the number of root surface female insects after 21 days of infection are reduced by 25% and 28.9% respectively. The soybean cystnematode Hg-flp-1 gene disclosed by the invention is applied to the field of soybean cyst nematode prevention and control.

The invention relates to a biocontrol strain SFC-3 for controlling nematode disease and application thereof, which can effectively solve that problem of unstable control effect and high use cost of existing biocontrol strain for controlling root-knot nematode diseases, and is classify and named as Trichoderma asperellum (CGMCC NO. 16097), which is a biocontrol strain SFC-3. At the concentration of1 * 109 CFU/mL, the egg hatching and the second instar larvae of root-knot nematode are inhibited. The strain can remarkably reduce the invasion of nematodes, and the control effect on root-knot nematode diseases is 78.51%. The strain can be used for irrigating roots when vegetable seedlings are transplanted, and has the growth promoting effect.

The invention relates to nematicidal-activity P. oxalicum NBC012 and a preparation method and application thereof. A high-activity fungus strain P. oxalicum NBC012 is isolated and screened from wheat cyst nematode group from stfold, Norway and is collected under CGMCC No. 12474. Nematicidal-activity P. oxalicum NBC012 is prepared by liquid fermentation culturing, fermentation broth of P. oxalicum NBC012 has significant poisoning action for plant parasitic nematodes. Indoor determination shows that the fermentation broth of the strain has effect on age-2 larvae and egg hatching of soybean cyst nematode. Fermentation broths with different dilution concentrations all show poisoning action for age-2 larvae and significant inhibitory action for egg hatching. Fermentation original broth may cause a decrease of 57.45% in amount of soybean cyst, and P. oxalicum NBC012 has a promising development and application prospect.

The invention discloses a molecular marker method for cerambycidae larvae of different families. The method is realized by the steps of: selecting two pairs of specific primers to carry out PCR reaction, and extracting genomic DNA from to-be-detected cerambycidae individuals and parents of different families by DNA extraction; taking the genomic DNA as the template, and carrying out two rounds of PCR amplification on the selected two pairs of primers respectively; subjecting the PCR product to 1.5% agarose electrophoresis detection, performing ethidium bromide staining, then conducting observation under ultraviolet, and photographing the electrophoresis result; and constructing a phylogenetic tree by a neighbor-joining method. According to the method provided by the invention, the genetic distances of 33 cerambycidae COI genes indicate that: the genetic distance between different genuses is obviously larger than that of a same genus, the COI gene is in full compliance with the inspection standard of DNA bar code validity, and the fragment can well distinguish different species of cerambycidae's different genuses, and can be used for rapid identification of cerambycidae.

The invention provides an application of an RNA interference technology in researching a target gene in a shellfish larva metamorphosis development process. Specifically, sinonovacula constricta is selected as a research object; dopamine beta-hydroxylase is used as a target gene; siRNA is designed, a method for soaking larvae in vitro by adopting siRNA is employed. The metamorphosis rate, the survival rate and the target gene expression quantity change of larvae are detected; namely, in the sinonovacula constricta larva development period, siRNA effectively reduces the expression level of target genes, the metamorphosis rate and the survival rate of the larvae are remarkably reduced, so that the feasibility of the in-vitro siRNA soaking method is indicated; the in-vitro soaking method adopted by the invention breaks through the limitation of injecting siRNA in vivo, and realizes the application of an RNA interference technology in shellfish larva research; the experimental operation issimple and easy to implement, is not limited by experimental conditions, and can eliminate the interference of other genes, thereby more accurately implementing the research on the single target gene.

The invention relates to a root-knot nematode lure attraction agent and application thereof, and belongs to the technical field of plant disease prevention and treatment. The biological lure attraction agent is obtained from Bacillus methylotrophicus Mo-TB strain with the culture preservation number being CGMCC No.11851 through the steps of test tube slant seed culture, liquid culture, fermentation pot mass culture and lure attraction agent preparation. The lure attraction agent is applied to lure attraction of root-knot nematode second-stage larvae in soil. The biological lure attractive agent and abamectin and fosthiazate chemical pesticides are mixed and then applied in prevention and treatment of the root-knot nematode disease. The root-knot nematode lure attraction agent has the advantages that the lure attraction rate of the biological lure attraction agent for root-knot nematode second-stage larvae in soil within one centare is 80% or above; the effect of preventing and treating the root-knot nematode disease is improved due to the fact that the biological lure attraction agent is used by being mixed with abamectin and fosthiazate chemical pesticides; the biological lure attraction agent is low in cost, free of residue and safe for human and livestock, and the prevention effect of conventional nematode killing pesticide can be significantly improved.

The invention belongs to the technical field of agricultural pest control, and particularly relates to a method for controlling pine wood nematode disease. The invention discloses a method for preventing and treating pine wood nematodes. On the basis of a scheme for preventing and treating pine wood nematode disease by using ethyl palmitate in the prior art; fluorite nano powder modified by juiceof selected succulent plants is added into ethyl palmitate; under the condition that the application amount of the low-dosage ethyl palmitate of the pine tree body is 0.5 to 5mu l/m <3>; the diapauserate of the pine wood nematodes is further improved, a large number of pine wood nematodes stop breeding, diffusion type pine wood nematodes (including diffusion type three-year-old larvae (LIII) anddiffusion type four-year-old larvae (LIV)) are formed, propagation of vector insects during eclosion of monochamus alternatus in January to May of the next year is reduced, and pine wood nematode diseases can be effectively prevented.

The invention provides an arthrobotrys oligospora strain N, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.5508. The strain is a mutagenic strain obtained by injecting low-energy nitrogen ion beams into arthrobotrys oligospora. The strain has the characteristics that the growth and reproduction are quick, spore production is early, the quantity of produced spores is large, and the capacity for catching and feeding on third-stage larva of nematode is strong, so that the strain can be used for biological control on livestock parasitic nematode.

The invention relates to a movable water treatment device for circulating water larva rearing of macrobrachium rosenbergii. The device comprises a tank shell, a water pump, a coarse filter, an ultraviolet lamp sterilizer, a biofilter, an aerator and a timer. The water pump, the coarse filter, the ultraviolet lamp sterilizer and the biofilter are connected through a pipeline and form a water circulating loop with a larva rearing pond outside the device, the aerator is serially connected with the biofilter, and the timer is connected with the water pump, the ultraviolet lamp sterilizer and the aerator. The movable water treatment device which is an intelligent water treatment device is small in size, easy to move, convenient, effective and applicable to macrobrachium rosenbergii larva rearing water treatment without circulating water devices, water environment improvement and energy consumption reduction can be realized, water change frequency is reduced, water resource recycling efficiency is improved, drug intake is reduced, larva diseases are prevented, and the survival rate of macrobrachium rosenbergii larvae is increased effectively.

The invention discloses a method for artificial mass reproduction of Protaetia brevitarsis larvae. The method comprises the following steps: (1) site selection; (2) preparation of an egg laying substrate; (3) assembly of a standard egg laying box; (4) placement of adult feed; (5) adult assembly and protective net arrangement; (6) egg collection every 6 days; and (7) hatching. The method for artificial mass propagation of Protaetia brevitarsis larvae disclosed by the invention has the following beneficial effects: 1, the reproduction cost is reduced; 2, the high reproduction efficiency is achieved; 3, the method is easy to operate and implement; and 4, the Protaetia brevitarsis larvae are reproduced for feeding on field waste, so that the energy utilization ratio can be improved.