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53 results about "Dauer larva" patented technology

Dauer (German "die Dauer", English "the enduring", "the duration" in the meaning of "a length of time", from A.G. Fuchs (1937) Neue parasitische und halbparasitische Nematoden bei Borkenkäfern und einige andere Nematoden, English New Parasitic and Half-parasitic Nematodes with Bark-Beetles and Some Other Nematodes) describes an alternative developmental stage of nematode worms, particularly rhabditids including Caenorhabditis elegans, whereby the larva goes into a type of stasis and can survive harsh conditions. Since the decision to enter the dauer stage is dependent on environmental cues, it represents a classic and well studied example of polyphenism. The Dauer state is given other names in the various types of nematodes such as ‘diapause’ or ‘hypobiosis’, but since the C. elegans nematode has become the most studied nematode, the term ‘dauer stage’ or 'dauer larvae' is becoming universally recognised when referring to this state in other free-living nematodes. The dauer stage is also considered to be equivalent to the infective stage of parasitic nematode larvae.

Nematicidal streptomyces JXSY860 and application thereof

The invention discloses nematode-killing streptomyces JXSY860 and application thereof, and belongs to the technical field of microbial pesticides. According to the invention, a new strain of Streptomyces turiscabs JXSY860 is found, the new strain of Streptomyces turiscabs JXSY860 has been preserved in Guangdong Microbial Culture Collection Center on August 8, 2024, and the preservation number is GDMCC No: 64998. The invention also discloses a preparation method of the Streptomyces turiscabs JXSY860. Through verification, the JXSY860 strain provided by the invention has stronger lethal activity on second-stage larvae of root-knot nematode, has stronger inhibition effect on incubation of root-knot nematode eggs, has important application value on prevention and control of root-knot nematode diseases of crops, and can be widely applied to preparation of biological agents for preventing and controlling the root-knot nematode diseases.
Owner:江西省农业科学院农业应用微生物研究所

Arthrobacter pasenii and application thereof

The invention relates to the technical field of prevention and control of microorganisms and nematodes, in particular to arthrobacter pastoris and application thereof. The arthrobacter pastoris disclosed by the invention is classified and named as Arthrobacter pascens, the strain number of the arthrobacter pastoris is YCH-T2, the arthrobacter pastoris is preserved in the China General Microbiological Culture Collection Center on March 19, 2024, and the preservation number of the arthrobacter pastoris is CGMCC No.30072. The strain has strong lethal activity on second-stage larvae of the root-knot nematode, has strong inhibition effect on incubation of eggs of the root-knot nematode, and has important application value in prevention and treatment of root-knot nematode diseases of crops.
Owner:SHAANXI INST OF BIOLOGICAL AGRI

DsRNA for preventing and treating liriomyza sativae and pesticide compound thereof

ActiveCN121065189ABiocideAnimal repellantsBiotechnologyChitin metabolism
The invention provides dsRNA for preventing and controlling liriomyza sativae and a pesticide compound thereof, the pesticide compound is formed by mixing dsRNA and an SPc star-type cation nano-carrier according to the mass ratio of 1: 1, and a nano-platform is formed through self-assembly and is used for targeted delivery of double-stranded RNA (dsRNA). The compound provided by the invention can significantly reduce the degradation rate of dsRNA in a plant leaf surface environment, can penetrate through a plant leaf cuticle and enter mesophyll tissue, so that larvae latent in the leaf can intake the compound, and the dsRNA targeted to LtCht2 is designed to specifically cut LtCht2 mRNA through the RNA interference pathway effect in the larva body, so that chitin metabolism disorder is caused, and the expression of the LtCht2 in the larva body is inhibited. Therefore, normal pupation and eclosion of the larvae are blocked, and finally the purpose of controlling the population of the liriomyza clover is achieved.
Owner:CHINA AGRI UNIV SANYA RES INST

Brevundimonas sp. Fermentation medium and application thereof in prevention and treatment of plant root-knot nematodes

The invention discloses a brevundimonas sp. Fermentation medium and application thereof in prevention and treatment of plant root-knot nematodes. Each liter of the brevundimonas chinensis fermentation medium comprises the following components by mass or volume: 6-10 g / L of yeast powder, 16-18 g / L of peptone, 31-45 ml / L of nematode lysis buffer, 0.8-2.5 g / L of chitin and 5.6-6 g / L of yeast extract, and the pH value of the brevundimonas chinensis fermentation medium is 6.5-6.8. The application of the fermentation broth culture medium refers to the application of the obtained fermentation broth in prevention and treatment of plant root-knot nematodes. According to the present invention, with the fermentation medium, the fatality rate of the obtained fermentation broth on the plant root-knot nematode exceeds 95%, the action time does not exceed 12 h, the nematode can be rapidly killed before the larva enters the plant, and the good application prospect is provided in the plant root-knot nematode prevention and control.
Owner:YUNNAN UNIV

Bacillus thuringiensis strain and use thereof

PendingCN122357354ABacillus thuringiensisAureobasidium sp.
The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.
Owner:INSTITUTE OF VEGETABLES & FLOWERS CHINESE ACADEMY OF AGRICULTURAL SCIENCES +1

Pseudomonas asiatica hnhmp s-260 and application thereof

PendingCN122104488ABiocidePlant growth regulatorsBiotechnologyStem length
The application relates to the field of microbial technology and discloses a Pseudomonas asiatica HNHMPs-260 and application thereof. The strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66854. Fermentation supernatant of the strain can prevent and treat root-knot nematode disease of Hami melon caused by Meloidogyne javanica. Experiments show that the fermentation supernatant has strong toxic effects on second instar larvae of root-knot nematodes and can significantly inhibit the hatching of egg masses and egg grains. In addition, the fermentation supernatant has good thermal stability and storage stability, and still maintains more than 80% nematocidal activity after high-temperature treatment at 121 DEG C and long-term storage at 4 DEG C. Meanwhile, the fermentation supernatant of the strain can promote the growth of Hami melon plants, improve physiological indexes such as root length, stem length, fresh weight and dry weight, and has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.
Owner:INST OF PLANT PROTECTION HAINAN ACAD OF AGRI SCI (HAINAN ACAD OF AGRI SCI AGRI PROD QUALITY SAFETY & STANDARDS RES CENT)

HparGR22 gene and application thereof in establishing phlorizin recognition in-vitro model

The invention is suitable for the technical field of insect genetic engineering, and provides an HparGR22 gene and application thereof in establishing a phlorizin recognition in-vitro model. The DNA sequence of the HparGR22 gene is as shown in SEQ ID No: 1, and the coded amino acid sequence is as shown in SEQ ID No: 2. The gene has the highest expression quantity in the lower labial tassel of a holotrichia parallela larva, an oocyte injected with cRNA of the gene has obvious response to stimulation of phlorizin with different concentrations, it is clear that a product expressed by the HparGR22 gene can recognize phlorizin, and the function of the HparGR22 gene as the phlorizin receptor gene of the holotrichia parallela is determined. In view of the fact that the phlorizin recognition function of the gene is clear for the first time, a basis is provided for application of the gene in establishment of a phlorizin recognition in-vitro model, a foundation is laid for exploring the potential value of the gene in the field of gene engineering for preventing and controlling holotrichia parallela, and meanwhile a new direction is opened up for further research and application of the gene.
Owner:JILIN UNIVERSITY

Rapid detection primer group for larvae and insect body fragments of bark beetles and application of rapid detection primer group

The invention relates to the technical field of molecular biological detection, in particular to a rapid detection primer group for bark beetle larvae and insect body fragments and application of the rapid detection primer group. According to the LAMP detection primer disclosed by the invention, the COI gene segment of the euphorbia leucopoda is used as a target gene for LAMP detection to design the LAMP detection primer, and the LAMP primer is high in specificity and high in sensitivity. On the basis, the invention provides the method for detecting the pachytous salicina, the rapid and accurate detection of the pachytous salicina in non-laboratory places such as ports and fields is realized by utilizing an LAMP technology, and the detection method has a wide application prospect.
Owner:INSPECTION & QUARANTINE TECH CENT OF NINGBO ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Method for rapidly identifying ecotype of small gold moth

The invention belongs to the field of identification of small gold moth, and particularly relates to a method for rapidly identifying ecotype of small gold moth. The method comprises the following steps: detecting the genotype of a Chr04.g05558 gene segment in a small gold moth genome, and determining the ecotype of the small gold moth according to the genotype; the Chr04. G05558 gene segment is located among Chr04: 51, 430, 521-51, 430 and 805 of the small gold moth, the Chr04. G05558 gene segment as shown in SEQ ID NO: 2 is a high mountain type small gold moth, and the Chr04. G05558 gene segment as shown in SEQ ID NO: 3 is a low mountain type small gold moth. The method disclosed by the invention can be used for identifying the ecotype of the small gold moth larva, is suitable for trace sample analysis, can directly interpret the result through conventional agarose gel electrophoresis, and can further judge the genotyping result through sequencing.
Owner:CHONGQING ACAD OF CHINESE MATERIA MEDICA

Compound moxidectin pour-on, preparation method and application thereof

PendingCN122320870AGastrointestinal nematodeMoxidectin
The present application relates to a kind of compound moxidectin pour-on and its preparation method and application, specifically to a kind of compound moxidectin pour-on, component mass mass fraction ratio includes: 0.1-5 portion moxidectin, 5-30 portion benzimidazole anthelmintic, 30-70 portion solvent, 5-20 portion solubilizer, 0.1-2 portion antioxidant, 10-30 portion transdermal enhancer.The present application not only covers gastrointestinal nematode, tapeworm, fluke and other various parasites, but also has killing or inhibiting effect on larva, worm egg, solve the problem of single component anthelmintic spectrum narrow, specific parasite effect is not good.
Owner:CHINA AGRI UNIV

Mortierella elongata and application thereof

PendingCN121427691ABiocideFungiMycangiumMicrobiological culture
The invention discloses Mortierella elongata and application of the Mortierella elongata, the strain number of the Mortierella elongata is JSAFC 247, the Mortierella elongata is preserved in the China General Microbiological Culture Collection Center on December 9, 2024, and the preservation number is CGMCC No.41703. The Mortierella elongata has the advantages that the Mortierella elongata can be used for preparing the Mortierella elongata and the application of the Mortierella elongata; the mortierella elongata strain provided by the invention is strong in pathogenicity to larvae of the larvae of the corpulenta aeruginosa, is environment-friendly and pollution-free, is not easy to generate drug resistance, can be used as a living body biopesticide, and has very strong application potential in biological prevention and control of the corpulenta aeruginosa.
Owner:BEIJING FORESTRY UNIVERSITY +1

Fermentation liquor of raw land combined with aspergillus niger and application thereof

The application discloses a kind of raw soil combined with fermentation liquor of aspergillus niger, and is the fermentation liquor obtained by fermenting aspergillus niger XO-2 on raw soil, specifically, aspergillus niger XO-2 is inoculated on raw soil to carry out fermentation, after fermentation, filtration, and the filtrate is the fermentation liquor of raw soil combined with aspergillus niger.The application separates and screens a strain of aspergillus niger from soil, and the fermentation liquor obtained by combining the aspergillus niger XO-2 with raw soil has a good toxic effect on second instar larvae of meloidogyne hapla chitwood, and can inhibit the hatching of eggs, which is of great significance for enriching plant parasitic nematode biocontrol bacteria resources and developing nematicidal plant and microbial source pesticides or pesticide fertilizers.
Owner:GUANGXI UNIV

Application of RAD9A gene as target spot in preparation of insect control preparation

The invention discloses application of an RAD9A gene as a target spot in preparation of a preparation for preventing and treating insects. The RAD9A gene of the plutella xylostella is knocked out by utilizing a CRISPR-Cas9 technology, and four kinds of homozygous mutants are obtained through screening. Wherein most of homozygous mutant embryos are lethal, and cavities of surviving homozygous mutant larva testis are increased and fused, and structural deformity such as whole atrophy of the testis occurs. Development of progenies of the surviving homozygous mutants is delayed, and the progenies finally die According to the invention, a UAS-GAL4 system is utilized to construct a fruit fly strain of specific RNAi RAD9A in a testis. Reduction of RAD9A expression can obviously cause apoptosis of drosophila larva testis cells, which indicates that RAD9 has a conservative function in insect testis development. Based on the ORF sequence conservative property of the insect RAD9A, the mutation of the region can be used as a control target of various pests. Based on the CRISPR-Cas9 technology, the RAD9A knockout target spot designed in the invention can play a similar effect on the lepidoptera insects.
Owner:ZHEJIANG UNIV

Bacillus licheniformis-derived peptide-1 and application thereof in prevention and control of root-knot nematode disease

The application discloses a bacillus licheniformis-derived nematocidal peptide Peptide-1 and application of the nematocidal peptide Peptide-1 in the prevention and control of root-knot nematode diseases, wherein the amino acid sequence of the nematocidal peptide Peptide-1 is shown as SEQ ID NO. 1. The application further provides a medicament for preventing and controlling root-knot nematodes and a prevention and control method thereof. The activity verification result shows that under the condition of 1.00 mmol / L, the corrected mortality of the nematocidal peptide Peptide-1 to the second instar larvae of root-knot nematodes reaches 90.7% after 24 hours, and the nematocidal peptide Peptide-1 has a clear and efficient lethal effect and concentration dependence. The nematocidal peptide Peptide-1 can be used for the green prevention and control of root-knot nematodes and the creation of new germplasm resources of root-knot nematode-resistant crops, meets the requirements of green agricultural development, and can effectively solve the problems of pesticide residues, drug resistance and ecological pollution caused by traditional chemical nematocidal agents.
Owner:HUAZHONG AGRI UNIV

Device and method for repelling longhorn beetle larvae in trunk by utilizing high-voltage electric shock principle

The invention provides a device for repelling longhorn beetle larvae in a trunk by using a high-voltage electric shock principle, which comprises a power supply connected with a high-voltage generator through a connecting wire; a frequency adjusting knob is mounted on the high-voltage generator, the high-voltage generator is respectively connected with the electrode probe and the loop electrode through connecting wires, a control switch is mounted on the connecting wire of the high-voltage generator and the electrode probe, and the electrode probe and the loop electrode are inserted into a tree trunk during use. High-voltage electric shock directly acts on insect channels in trunks, current penetrates through xylem, accurate electric shock killing of longhorn beetle larvae is achieved, the defects that chemical pesticides are difficult to permeate the trunks and do not act directly are overcome, the prevention and treatment efficiency is remarkably improved, and the prevention and treatment cost is reduced. And the problems of insufficient ultrasonic penetrating power, inapplicable power grid structure and the like in the prior art are solved.
Owner:贵阳市城市绿化服务中心

Use of a nuclear receptor target gene in preparation of a biological agent for blocking activation of diapause larvae

ActiveCN121780548BNucleic acid vectorAntiparasitic agentsBiotechnologyProgesterone/Estradiol
The application discloses an application of a nuclear receptor target gene in preparation of a biological preparation for blocking activation of diapause larvae, and relates to the field of biotechnology and parasite prevention and control. According to two nuclear receptor targets of Haemonchus contortus HCON_00101910 (regulating activity of worms) and HCON_00023750 (regulating development of larvae), the two nuclear receptor targets are combined with host progesterone and estradiol and are activated, constitute a key signal path in the spring activation process of diapause larvae, and have no homologous genes in mammals, and the safety is excellent. By constructing specific shRNA lentivirus vectors (the silencing efficiency is all greater than or equal to 60%) targeting the two genes, single target or double target synergistic intervention is realized, and the activity-development double key links of worms in the host body can be specifically blocked. The application provides a new paradigm of preventive prevention and control with high specificity and green safety, and provides core technical support for solving the seasonal epidemic problem of blood fluke disease.
Owner:ZHEJIANG UNIV

Westersic monocytogenes for preventing and treating root-knot nematode and application thereof

The application discloses a huaxi brevitoxa for preventing and treating root-knot nematodes and application thereof. The huaxi brevitoxa (preserved in the Guangdong Microbial Digital Culture Collection Center, numbered as GDMCC No: 65867, and the preservation address is No. 100, Martyr Road, Yuexiu District, Guangzhou City, Guangdong Province) can not only attract nematodes but also kill nematodes, can actively attract nematodes to the pesticide action area, concentrate the nematodes for killing, improve the prevention and treatment effect, and can avoid the waste of traditional pesticide spraying in a large range, reduce the pesticide usage, reduce the cost, and reduce the environmental pollution. The lethal rate of the huaxi brevitoxa fermentation liquor to the root-knot nematodes is more than 89%, the lethal rate of the 50-fold diluted fermentation liquor to the root-knot nematodes is more than 80%, the action time is not more than 12 hours, the nematodes can be quickly killed before the larvae enter the plant, and the huaxi brevitoxa fermentation liquor has a good application prospect in the prevention and treatment of plant root-knot nematodes.
Owner:YUNNAN UNIV

A parsonomyces sp. and applications thereof

The present application relates to the technical field of microorganism and nematode control, and particularly relates to a joint arthrobacter parsonii and application thereof. Arthrobacter pascens The joint arthrobacter parsonii of the present application is classified as Arthrobacter parsonii, and the strain number is YCH-T2, which has been preserved in the China General Microbiological Culture Collection Center on March 19, 2024, and the preservation number is CGMCC No.30072. The strain has strong lethal activity on the second instar larvae of root-knot nematodes, and has strong inhibitory effect on the hatching of root-knot nematode eggs, and has important application value for the control of crop root-knot nematode disease.
Owner:SHAANXI INST OF BIOLOGICAL AGRI

Arthrobacter sp. ZJUHUYJH1 and application thereof in preventing and treating gramineae meloidogyne

ActiveCN120738008ABiocideBacteriaBiotechnologyArthrobacter
The invention provides arthrobacter ZJUHUYJH1 and application thereof in preventing and treating gramineous meloidogyne, and belongs to the technical field of prevention and treatment of plant meloidogyne. The arthrobacter ZJUHUYJH1 provided by the invention has the preservation number of CCTCC NO: M 2025922, is separated from rice root soil infected by the gramineous meloidogyne, and has the advantages that the preservation number is CCTCC NO: M 2025922; the composition has a good prevention and treatment effect on the gramineous meloidogyne; the correction fatality rate of second-stage larvae of the meloidogyne reaches 91.4%, and the root knot number and the ratio of the root knot number to the dry weight of rice infected by the pseudogramineous meloidogyne can be reduced; the method has potential and practical application value in research of the Meloidogyne pseudoaceae.
Owner:ZHEJIANG UNIV

Soil-borne disease biocontrol inoculant and preparation method and application thereof

The invention belongs to the technical field of biocontrol microbial agents, and particularly relates to a soil-borne disease biocontrol microbial agent as well as a preparation method and application thereof. The soil-borne disease biocontrol microbial inoculum is prepared by compounding a bacillus velezensis fermentation broth and a brevibacillus beautiful fermentation broth according to a volume ratio of 1: (0.5-1.5). Wherein the fermentation liquor contains thalli. The bacillus velezensis is a YCH-H7 strain, the preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) No.33510, and the preservation date of the bacillus velezensis is February 11, 2025. The brevibacillus beautiful is an NF2 strain. The corrected death rate of second-instar larvae treated by the soil-borne disease biocontrol microbial inoculum for 48 hours is 98.85%, the nematode egg incubation inhibition rate is 88%, and the corrected death rate of ditylenchus destructor treated for 4 days is 97.7%. The hypha growth inhibition rates of the soil-borne disease biocontrol microbial inoculum on kiwi fruit root rot pathogenic bacteria, pepper phytophthora blight pathogenic bacteria, tomato neck and root rot, cucumber root rot pathogenic bacteria and wheat stem rot pathogenic bacteria exceed 90%, and the soil-borne disease biocontrol microbial inoculum has obvious broad spectrum.
Owner:SHAANXI INST OF BIOLOGICAL AGRI

Acetylcholine receptor unc38 gene of ceratobius gigas and dsrna and application thereof

PendingCN122382078ABiotechnologyAcetylcholine receptor
This invention belongs to the field of biotechnology, specifically relating to the acetylcholine receptor of the root-knot nematode *Eupolyphaga sinensis*. unc38 Genes, their dsRNAs, and applications. To provide novel target genes for the control of *Eriocheir sinensis* root-knot nematode, this invention is based on the *Eriocheir sinensis* acetylcholine receptor... unc38 Gene SEQ ID NO:1 was used to design and synthesize gene fragment SEQ ID NO:5 and the corresponding dsRNA. The synthesized dsRNA was used to soak second-instar root-knot nematodes. unc38 Gene expression levels were downregulated, resulting in a mortality rate of 68.9% at 60 hours, and complete death of nematodes at 120 hours. Immersion unc38 The dsRNA-infected second-instar larvae of the bean root-knot nematode significantly reduced the infectivity of tomato roots. After 30 days of inoculation, the number of root knots decreased significantly by about 57%, and the number of females, the number of egg masses, the number of eggs in each egg mass, and reproductive factors were all significantly reduced.
Owner:SHANXI UNIV +1

Application of sNPF gene and inhibitor thereof in prevention and control of beet armyworm

The invention discloses an sNPF gene and application of an inhibitor of the sNPF gene in prevention and control of beet armyworms, and belongs to the technical field of biology. The sNPF gene of the beet armyworm is silenced through an RNA interference technology, and the sNPF is found to play an important regulation role in ingestion, growth and development and energy metabolism of larvae; the sNPF gene silencing obviously reduces the feeding amount and the body weight growth rate of the larvae, and increases the death rate of the larvae under hunger stress; in addition, silence of the sNPF gene also significantly affects the content of glycometabolism substances in the larva body. The sNPF is found to be a key factor for regulating and controlling the physiological process of beet armyworm larvae, has the potential of serving as a new target for pest control, and provides a theoretical basis for prevention and control of beet armyworms.
Owner:SHANGHAI ACAD OF AGRI SCI

Arthrobacter ZJUHUYJH1 and application thereof in prevention and treatment of meloidogyne chitwoodi

ActiveCN120738008BIncrease resistancereduce overdependenceBiocideBacteriaBiotechnologyArthrobacter
The application provides a strain of Arthrobacter sp. ZJUHUYJH1 and application thereof in prevention and treatment of paratyphaceae root-knot nematodes, and belongs to the technical field of prevention and treatment of plant root-knot nematodes. The Arthrobacter sp. ZJUHUYJH1 provided by the application has a preservation number of CCTCC NO: M 2025922, is isolated from rice rhizosphere soil infected by paratyphaceae root-knot nematodes, has a good prevention and treatment effect on paratyphaceae root-knot nematodes, a rectification mortality of 91.4% on second instar larvae of root-knot nematodes, can reduce the number of root-knots and the ratio of the number of root-knots to dry weight of rice infected by paratyphaceae root-knot nematodes, and has potential and practical application value in the research of paratyphaceae root-knot nematodes.
Owner:ZHEJIANG UNIV

Strain BtJC2604 with high activity on phyllotreta striolata and application

The invention relates to a strain BtJC2604 with high activity on phyllotreta striolata and application of the strain BtJC2604. The bacillus thuringiensis wild strain BtJC2604 is screened from the environment by the applicant, and the preservation number of the bacillus thuringiensis wild strain BtJC2604 is CGMCC (China General Microbiological Culture Collection Center) No.36685 The strain BtJC2604 provided by the invention has activity on phyllotreta striolata larvae, and realizes larva control on phyllotreta striolata. The activity of the strain BtJC2604 provided by the invention on larvae is higher than that of the existing strain G033A.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI

Meloidogyne hapla-like class neural peptide nlp12 gene, dsrna and application thereof

PendingCN122629069ABiotechnologyNucleotide
This invention belongs to the field of biotechnology, specifically relating to neuropeptides from the root-knot nematode *Euonymus alatus*. nlp12 Genes, their dsRNAs, and applications. To provide novel target genes for the control of *Heliotropium indicum* root-knot nematodes, this invention is based on... nlp12 The nucleotide sequence of the gene is SEQ ID NO:1. Gene fragment SEQ ID NO:5 and the corresponding dsRNA were designed and synthesized. The synthesized dsRNA was used to soak second-instar root-knot nematodes. nlp12 Gene expression levels were downregulated, resulting in a mortality rate of 73.2% at 60 hours, and complete mortality of nematodes at 108 hours. Immersion nlp12 The dsRNA-infected second-instar larvae of the bean root-knot nematode significantly reduced the infectivity of tomato roots. After 30 days of inoculation, the number of root knots decreased significantly by about 54%, and the number of female nematodes, the number of egg masses, the number of eggs in each egg mass, and reproductive factors were all significantly reduced.
Owner:SHANXI UNIV +1

A pine nematode inhibiting method based on pachyman substitute cultivation

The application discloses a pine nematode inhibition method based on pachyman substitute cultivation, and comprises the steps of destroying the survival carrier of pine wood nematode and intermediate host, substitute fermentation directional regulation of microbial environment and pachyman fungus biological control, wherein the substitute fermentation makes the pH of the substrate in the range of 5.0-6.5, and the microbial environment for inhibiting the hatching of pine wood nematode and monochamus alternatus egg, the development of larva and the reproduction of adult is constructed; especially, the pH of the substitute substrate after fermentation is maintained at 5.5-6.5, and the pachyman fungus is combined. Compared with the existing biological control method of diseased wood, the pine nematode inhibition rate can be increased from 60-70% to 100%, and the secondary transmission risk of pine nematode is significantly reduced. While realizing the harmless treatment of diseased wood and completely blocking the transmission of pine nematode, efficient and high-quality cultivation of pachyman is achieved, and the method is suitable for the large-scale treatment of pine wood nematode disease and the industrialized planting of pachyman genuine producing area.
Owner:HUBEI PROVINCIAL HOSPITAL OF TRADITIONAL CHINESE MEDICINE (AFFILIATED HOSPITAL OF HUBEI UNIV OF TRADITIONAL CHINESE MEDICINE HUBEI INST OF TRADITIONAL CHINESE MEDICINE)

Application of cypermethrin in prevention and control of monochamus alternatus hope and interception of propagation of pine wood nematodes

The invention relates to application of cypermethrin in prevention and control of monochamus alternatus and interception of propagation of pine wood nematodes. According to the implementation method in the application, the cypermethrin emulsion in water is mainly sprayed to the tail section of withered and dead wood, and the cypermethrin emulsion in water can also be sprayed to branches of a host pinus massoniana in an auxiliary mode. Cypermethrin not only can effectively kill monochamus alternatus larvae in pine wood, inhibit the development of the larvae and eclosion of the monochamus alternatus larvae and block the propagation and diffusion path of pine wood nematode diseases, but also has potential application value in the aspect of treatment of epidemic wood in epidemic disease areas.
Owner:ZHEJIANG FORESTRY UNIVERSITY +1

A dsRNA nanoformulation and its application in the control of red turpentine beetle.

PendingCN122503381ADendroctonus valensTurpentine
This invention belongs to the field of forestry biotechnology, specifically relating to a dsRNA nanoparticle formulation and its application in the control of the red turpentine beetle. This invention utilizes RNA interference technology to effectively control the red turpentine beetle by interfering with the expression of its V-ATPase gene. To optimize application methods and improve ease of use, this invention uses carbon quantum dots (CQDs) to encapsulate the dsRNA to prepare a nanoparticle formulation. This nanoparticle formulation can also silence the V-ATPase gene and cause abnormal death in red turpentine beetle larvae. This invention utilizes RNAi technology to specifically target the red turpentine beetle, offering advantages such as strong targeting, environmental friendliness, and low risk of developing resistance. It solves the problems of environmental pollution and increased pest resistance associated with existing chemical pesticide control of the red turpentine beetle, providing a new technical approach and material basis for the green and efficient control of the red turpentine beetle, and has significant application value and economic and ecological benefits.
Owner:ANHUI AGRICULTURAL UNIVERSITY

A gene editing method for obtaining a double-shelled bivalve adult edited body

The present application belongs to the technical field of gene editing, and particularly relates to a gene editing method for obtaining a bivalve adult editing body. Specifically, a target gene with an outward visible phenotype and an early development not lethal is taken as a marker, target sites sgRNAs are designed based on the marker, and then a Cas9 / sgRNAs editing body is constructed by means of an electrotransfection system, so that gene editing of the bivalve is realized and the adult editing body is obtained. The present application breaks through the technical bottleneck that the existing gene editing of the bivalve is limited to the larva development stage and the editing body is difficult to screen in adulthood, and provides a new technical system for creating the editing body germplasm of the bivalve. In addition to being applicable to oysters, the technical system is also applicable to other bivalves with small fertilized eggs, difficult microinjection and high larva culture mortality.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Application of butylated hydroxytoluene in enhancing biocontrol activity of entomopathogenic nematodes

The invention discloses an application of butylated hydroxytoluene in enhancing the biocontrol activity of entomopathogenic nematodes. The entomopathogenic nematodes are pretreated by using the volatile butylated hydroxytoluene specifically released by the larvae of the red weevil, so that the activity of the entomopathogenic nematodes can be remarkably enhanced, and the quantitative enhancement of the behavior of the nematodes can be achieved; therefore, the parasitic lethal ability of entomopathogenic nematodes to hosts (larvae such as red palm weevils, greater wax moths and tomato leaf miners) is improved, and the control effect on various pests is effectively improved. Butylated hydroxytoluene has no toxic or side effect on entomopathogenic nematodes under an effective concentration, so that the survival and activity stability of the entomopathogenic nematodes are ensured. The invention provides a safe, efficient and wide-application-range strategy for improving biological prevention and control means based on entomopathogenic nematodes, and has good popularization and application prospects.
Owner:FUJIAN AGRI & FORESTRY UNIV