This invention belongs to the field of
biotechnology, specifically relating to neuropeptides from the root-knot
nematode *
Euonymus alatus*. nlp12 Genes, their dsRNAs, and applications. To provide novel target genes for the control of *
Heliotropium indicum* root-knot nematodes, this invention is based on... nlp12 The
nucleotide sequence of the
gene is SEQ ID NO:1.
Gene fragment SEQ ID NO:5 and the corresponding dsRNA were designed and synthesized. The synthesized dsRNA was used to soak second-
instar root-knot nematodes. nlp12
Gene expression levels were downregulated, resulting in a
mortality rate of 73.2% at 60 hours, and complete mortality of nematodes at 108 hours. Immersion nlp12 The dsRNA-infected second-
instar larvae of the bean root-knot
nematode significantly reduced the
infectivity of tomato roots. After 30 days of
inoculation, the number of root knots decreased significantly by about 54%, and the number of female nematodes, the number of
egg masses, the number of eggs in each egg
mass, and reproductive factors were all significantly reduced.