34 results about "Dauer larva" patented technology

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The application relates to the field of microbial technology and discloses a Pseudomonas asiatica HNHMPs-260 and application thereof. The strain is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66854. Fermentation supernatant of the strain can prevent and treat root-knot nematode disease of Hami melon caused by Meloidogyne javanica. Experiments show that the fermentation supernatant has strong toxic effects on second instar larvae of root-knot nematodes and can significantly inhibit the hatching of egg masses and egg grains. In addition, the fermentation supernatant has good thermal stability and storage stability, and still maintains more than 80% nematocidal activity after high-temperature treatment at 121 DEG C and long-term storage at 4 DEG C. Meanwhile, the fermentation supernatant of the strain can promote the growth of Hami melon plants, improve physiological indexes such as root length, stem length, fresh weight and dry weight, and has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.

The invention belongs to the field of identification of small gold moth, and particularly relates to a method for rapidly identifying ecotype of small gold moth. The method comprises the following steps: detecting the genotype of a Chr04.g05558 gene segment in a small gold moth genome, and determining the ecotype of the small gold moth according to the genotype; the Chr04. G05558 gene segment is located among Chr04: 51, 430, 521-51, 430 and 805 of the small gold moth, the Chr04. G05558 gene segment as shown in SEQ ID NO: 2 is a high mountain type small gold moth, and the Chr04. G05558 gene segment as shown in SEQ ID NO: 3 is a low mountain type small gold moth. The method disclosed by the invention can be used for identifying the ecotype of the small gold moth larva, is suitable for trace sample analysis, can directly interpret the result through conventional agarose gel electrophoresis, and can further judge the genotyping result through sequencing.

The present application relates to a kind of compound moxidectin pour-on and its preparation method and application, specifically to a kind of compound moxidectin pour-on, component mass mass fraction ratio includes: 0.1-5 portion moxidectin, 5-30 portion benzimidazole anthelmintic, 30-70 portion solvent, 5-20 portion solubilizer, 0.1-2 portion antioxidant, 10-30 portion transdermal enhancer.The present application not only covers gastrointestinal nematode, tapeworm, fluke and other various parasites, but also has killing or inhibiting effect on larva, worm egg, solve the problem of single component anthelmintic spectrum narrow, specific parasite effect is not good.

The invention discloses Mortierella elongata and application of the Mortierella elongata, the strain number of the Mortierella elongata is JSAFC 247, the Mortierella elongata is preserved in the China General Microbiological Culture Collection Center on December 9, 2024, and the preservation number is CGMCC No.41703. The Mortierella elongata has the advantages that the Mortierella elongata can be used for preparing the Mortierella elongata and the application of the Mortierella elongata; the mortierella elongata strain provided by the invention is strong in pathogenicity to larvae of the larvae of the corpulenta aeruginosa, is environment-friendly and pollution-free, is not easy to generate drug resistance, can be used as a living body biopesticide, and has very strong application potential in biological prevention and control of the corpulenta aeruginosa.

The invention discloses application of an RAD9A gene as a target spot in preparation of a preparation for preventing and treating insects. The RAD9A gene of the plutella xylostella is knocked out by utilizing a CRISPR-Cas9 technology, and four kinds of homozygous mutants are obtained through screening. Wherein most of homozygous mutant embryos are lethal, and cavities of surviving homozygous mutant larva testis are increased and fused, and structural deformity such as whole atrophy of the testis occurs. Development of progenies of the surviving homozygous mutants is delayed, and the progenies finally die According to the invention, a UAS-GAL4 system is utilized to construct a fruit fly strain of specific RNAi RAD9A in a testis. Reduction of RAD9A expression can obviously cause apoptosis of drosophila larva testis cells, which indicates that RAD9 has a conservative function in insect testis development. Based on the ORF sequence conservative property of the insect RAD9A, the mutation of the region can be used as a control target of various pests. Based on the CRISPR-Cas9 technology, the RAD9A knockout target spot designed in the invention can play a similar effect on the lepidoptera insects.

The application discloses a bacillus licheniformis-derived nematocidal peptide Peptide-1 and application of the nematocidal peptide Peptide-1 in the prevention and control of root-knot nematode diseases, wherein the amino acid sequence of the nematocidal peptide Peptide-1 is shown as SEQ ID NO. 1. The application further provides a medicament for preventing and controlling root-knot nematodes and a prevention and control method thereof. The activity verification result shows that under the condition of 1.00 mmol / L, the corrected mortality of the nematocidal peptide Peptide-1 to the second instar larvae of root-knot nematodes reaches 90.7% after 24 hours, and the nematocidal peptide Peptide-1 has a clear and efficient lethal effect and concentration dependence. The nematocidal peptide Peptide-1 can be used for the green prevention and control of root-knot nematodes and the creation of new germplasm resources of root-knot nematode-resistant crops, meets the requirements of green agricultural development, and can effectively solve the problems of pesticide residues, drug resistance and ecological pollution caused by traditional chemical nematocidal agents.

The application discloses an application of a nuclear receptor target gene in preparation of a biological preparation for blocking activation of diapause larvae, and relates to the field of biotechnology and parasite prevention and control. According to two nuclear receptor targets of Haemonchus contortus HCON_00101910 (regulating activity of worms) and HCON_00023750 (regulating development of larvae), the two nuclear receptor targets are combined with host progesterone and estradiol and are activated, constitute a key signal path in the spring activation process of diapause larvae, and have no homologous genes in mammals, and the safety is excellent. By constructing specific shRNA lentivirus vectors (the silencing efficiency is all greater than or equal to 60%) targeting the two genes, single target or double target synergistic intervention is realized, and the activity-development double key links of worms in the host body can be specifically blocked. The application provides a new paradigm of preventive prevention and control with high specificity and green safety, and provides core technical support for solving the seasonal epidemic problem of blood fluke disease.

The application discloses a huaxi brevitoxa for preventing and treating root-knot nematodes and application thereof. The huaxi brevitoxa (preserved in the Guangdong Microbial Digital Culture Collection Center, numbered as GDMCC No: 65867, and the preservation address is No. 100, Martyr Road, Yuexiu District, Guangzhou City, Guangdong Province) can not only attract nematodes but also kill nematodes, can actively attract nematodes to the pesticide action area, concentrate the nematodes for killing, improve the prevention and treatment effect, and can avoid the waste of traditional pesticide spraying in a large range, reduce the pesticide usage, reduce the cost, and reduce the environmental pollution. The lethal rate of the huaxi brevitoxa fermentation liquor to the root-knot nematodes is more than 89%, the lethal rate of the 50-fold diluted fermentation liquor to the root-knot nematodes is more than 80%, the action time is not more than 12 hours, the nematodes can be quickly killed before the larvae enter the plant, and the huaxi brevitoxa fermentation liquor has a good application prospect in the prevention and treatment of plant root-knot nematodes.

The present application relates to the technical field of microorganism and nematode control, and particularly relates to a joint arthrobacter parsonii and application thereof. Arthrobacter pascens The joint arthrobacter parsonii of the present application is classified as Arthrobacter parsonii, and the strain number is YCH-T2, which has been preserved in the China General Microbiological Culture Collection Center on March 19, 2024, and the preservation number is CGMCC No.30072. The strain has strong lethal activity on the second instar larvae of root-knot nematodes, and has strong inhibitory effect on the hatching of root-knot nematode eggs, and has important application value for the control of crop root-knot nematode disease.

The invention belongs to the technical field of biocontrol microbial agents, and particularly relates to a soil-borne disease biocontrol microbial agent as well as a preparation method and application thereof. The soil-borne disease biocontrol microbial inoculum is prepared by compounding a bacillus velezensis fermentation broth and a brevibacillus beautiful fermentation broth according to a volume ratio of 1: (0.5-1.5). Wherein the fermentation liquor contains thalli. The bacillus velezensis is a YCH-H7 strain, the preservation number of the bacillus velezensis is CGMCC (China General Microbiological Culture Collection Center) No.33510, and the preservation date of the bacillus velezensis is February 11, 2025. The brevibacillus beautiful is an NF2 strain. The corrected death rate of second-instar larvae treated by the soil-borne disease biocontrol microbial inoculum for 48 hours is 98.85%, the nematode egg incubation inhibition rate is 88%, and the corrected death rate of ditylenchus destructor treated for 4 days is 97.7%. The hypha growth inhibition rates of the soil-borne disease biocontrol microbial inoculum on kiwi fruit root rot pathogenic bacteria, pepper phytophthora blight pathogenic bacteria, tomato neck and root rot, cucumber root rot pathogenic bacteria and wheat stem rot pathogenic bacteria exceed 90%, and the soil-borne disease biocontrol microbial inoculum has obvious broad spectrum.

This invention belongs to the field of biotechnology, specifically relating to the acetylcholine receptor of the root-knot nematode *Eupolyphaga sinensis*. unc38 Genes, their dsRNAs, and applications. To provide novel target genes for the control of *Eriocheir sinensis* root-knot nematode, this invention is based on the *Eriocheir sinensis* acetylcholine receptor... unc38 Gene SEQ ID NO:1 was used to design and synthesize gene fragment SEQ ID NO:5 and the corresponding dsRNA. The synthesized dsRNA was used to soak second-instar root-knot nematodes. unc38 Gene expression levels were downregulated, resulting in a mortality rate of 68.9% at 60 hours, and complete death of nematodes at 120 hours. Immersion unc38 The dsRNA-infected second-instar larvae of the bean root-knot nematode significantly reduced the infectivity of tomato roots. After 30 days of inoculation, the number of root knots decreased significantly by about 57%, and the number of females, the number of egg masses, the number of eggs in each egg mass, and reproductive factors were all significantly reduced.

The invention discloses an sNPF gene and application of an inhibitor of the sNPF gene in prevention and control of beet armyworms, and belongs to the technical field of biology. The sNPF gene of the beet armyworm is silenced through an RNA interference technology, and the sNPF is found to play an important regulation role in ingestion, growth and development and energy metabolism of larvae; the sNPF gene silencing obviously reduces the feeding amount and the body weight growth rate of the larvae, and increases the death rate of the larvae under hunger stress; in addition, silence of the sNPF gene also significantly affects the content of glycometabolism substances in the larva body. The sNPF is found to be a key factor for regulating and controlling the physiological process of beet armyworm larvae, has the potential of serving as a new target for pest control, and provides a theoretical basis for prevention and control of beet armyworms.

The application provides a strain of Arthrobacter sp. ZJUHUYJH1 and application thereof in prevention and treatment of paratyphaceae root-knot nematodes, and belongs to the technical field of prevention and treatment of plant root-knot nematodes. The Arthrobacter sp. ZJUHUYJH1 provided by the application has a preservation number of CCTCC NO: M 2025922, is isolated from rice rhizosphere soil infected by paratyphaceae root-knot nematodes, has a good prevention and treatment effect on paratyphaceae root-knot nematodes, a rectification mortality of 91.4% on second instar larvae of root-knot nematodes, can reduce the number of root-knots and the ratio of the number of root-knots to dry weight of rice infected by paratyphaceae root-knot nematodes, and has potential and practical application value in the research of paratyphaceae root-knot nematodes.

The invention relates to a strain BtJC2604 with high activity on phyllotreta striolata and application of the strain BtJC2604. The bacillus thuringiensis wild strain BtJC2604 is screened from the environment by the applicant, and the preservation number of the bacillus thuringiensis wild strain BtJC2604 is CGMCC (China General Microbiological Culture Collection Center) No.36685 The strain BtJC2604 provided by the invention has activity on phyllotreta striolata larvae, and realizes larva control on phyllotreta striolata. The activity of the strain BtJC2604 provided by the invention on larvae is higher than that of the existing strain G033A.

This invention belongs to the field of biotechnology, specifically relating to neuropeptides from the root-knot nematode *Euonymus alatus*. nlp12 Genes, their dsRNAs, and applications. To provide novel target genes for the control of *Heliotropium indicum* root-knot nematodes, this invention is based on... nlp12 The nucleotide sequence of the gene is SEQ ID NO:1. Gene fragment SEQ ID NO:5 and the corresponding dsRNA were designed and synthesized. The synthesized dsRNA was used to soak second-instar root-knot nematodes. nlp12 Gene expression levels were downregulated, resulting in a mortality rate of 73.2% at 60 hours, and complete mortality of nematodes at 108 hours. Immersion nlp12 The dsRNA-infected second-instar larvae of the bean root-knot nematode significantly reduced the infectivity of tomato roots. After 30 days of inoculation, the number of root knots decreased significantly by about 54%, and the number of female nematodes, the number of egg masses, the number of eggs in each egg mass, and reproductive factors were all significantly reduced.

The application discloses a pine nematode inhibition method based on pachyman substitute cultivation, and comprises the steps of destroying the survival carrier of pine wood nematode and intermediate host, substitute fermentation directional regulation of microbial environment and pachyman fungus biological control, wherein the substitute fermentation makes the pH of the substrate in the range of 5.0-6.5, and the microbial environment for inhibiting the hatching of pine wood nematode and monochamus alternatus egg, the development of larva and the reproduction of adult is constructed; especially, the pH of the substitute substrate after fermentation is maintained at 5.5-6.5, and the pachyman fungus is combined. Compared with the existing biological control method of diseased wood, the pine nematode inhibition rate can be increased from 60-70% to 100%, and the secondary transmission risk of pine nematode is significantly reduced. While realizing the harmless treatment of diseased wood and completely blocking the transmission of pine nematode, efficient and high-quality cultivation of pachyman is achieved, and the method is suitable for the large-scale treatment of pine wood nematode disease and the industrialized planting of pachyman genuine producing area.

The invention relates to application of cypermethrin in prevention and control of monochamus alternatus and interception of propagation of pine wood nematodes. According to the implementation method in the application, the cypermethrin emulsion in water is mainly sprayed to the tail section of withered and dead wood, and the cypermethrin emulsion in water can also be sprayed to branches of a host pinus massoniana in an auxiliary mode. Cypermethrin not only can effectively kill monochamus alternatus larvae in pine wood, inhibit the development of the larvae and eclosion of the monochamus alternatus larvae and block the propagation and diffusion path of pine wood nematode diseases, but also has potential application value in the aspect of treatment of epidemic wood in epidemic disease areas.

This invention belongs to the field of forestry biotechnology, specifically relating to a dsRNA nanoparticle formulation and its application in the control of the red turpentine beetle. This invention utilizes RNA interference technology to effectively control the red turpentine beetle by interfering with the expression of its V-ATPase gene. To optimize application methods and improve ease of use, this invention uses carbon quantum dots (CQDs) to encapsulate the dsRNA to prepare a nanoparticle formulation. This nanoparticle formulation can also silence the V-ATPase gene and cause abnormal death in red turpentine beetle larvae. This invention utilizes RNAi technology to specifically target the red turpentine beetle, offering advantages such as strong targeting, environmental friendliness, and low risk of developing resistance. It solves the problems of environmental pollution and increased pest resistance associated with existing chemical pesticide control of the red turpentine beetle, providing a new technical approach and material basis for the green and efficient control of the red turpentine beetle, and has significant application value and economic and ecological benefits.

The present application belongs to the technical field of gene editing, and particularly relates to a gene editing method for obtaining a bivalve adult editing body. Specifically, a target gene with an outward visible phenotype and an early development not lethal is taken as a marker, target sites sgRNAs are designed based on the marker, and then a Cas9 / sgRNAs editing body is constructed by means of an electrotransfection system, so that gene editing of the bivalve is realized and the adult editing body is obtained. The present application breaks through the technical bottleneck that the existing gene editing of the bivalve is limited to the larva development stage and the editing body is difficult to screen in adulthood, and provides a new technical system for creating the editing body germplasm of the bivalve. In addition to being applicable to oysters, the technical system is also applicable to other bivalves with small fertilized eggs, difficult microinjection and high larva culture mortality.

The invention discloses an application of butylated hydroxytoluene in enhancing the biocontrol activity of entomopathogenic nematodes. The entomopathogenic nematodes are pretreated by using the volatile butylated hydroxytoluene specifically released by the larvae of the red weevil, so that the activity of the entomopathogenic nematodes can be remarkably enhanced, and the quantitative enhancement of the behavior of the nematodes can be achieved; therefore, the parasitic lethal ability of entomopathogenic nematodes to hosts (larvae such as red palm weevils, greater wax moths and tomato leaf miners) is improved, and the control effect on various pests is effectively improved. Butylated hydroxytoluene has no toxic or side effect on entomopathogenic nematodes under an effective concentration, so that the survival and activity stability of the entomopathogenic nematodes are ensured. The invention provides a safe, efficient and wide-application-range strategy for improving biological prevention and control means based on entomopathogenic nematodes, and has good popularization and application prospects.

The invention belongs to the technical field of biopesticides, and discloses an Akanthomyces varioti strain biocontrol agent and application thereof in crop nematode disease prevention and control, the preservation number of the Akanthomyces varioti strain in China Center for Type Culture Collection is CGMCC No: 41597, the biocontrol agent is fermentation liquor containing the Akanthomyces varioti strain, the preservation number of the biocontrol agent in China Center for Type Culture Collection is CGMCC No: 41597, and the biocontrol agent can be applied to crop nematode disease prevention and control. The bactericidal composition can be applied to prevention and treatment of crop nematode diseases. The echinococcus caerulea biocontrol agent has a strong lethal effect on second-stage larvae (J2) of meloidogyne incognita and inhibits infection of the meloidogyne incognita J2 on plants. The biocontrol agent has the characteristics of high efficiency and low toxicity, can reduce the generation of nematode resistance to drugs, is beneficial to environmental protection, can reduce pesticide residues on agricultural products, and has a wide application prospect.

The invention discloses an LAMP (loop-mediated isothermal amplification) specific primer group for identifying corn cyst nematode and application of the LAMP specific primer group. The LAMP specific primer group can be used for identifying the corn cyst nematode. The method comprises the following steps: carrying out loop-mediated isothermal amplification by taking genome DNA (Deoxyribose Nucleic Acid) of a nematode to be detected as a template and adopting a specific LAMP primer group; after the reaction is finished, carrying out agarose gel electrophoresis on an amplification product, or carrying out SYBR Green I chromogenic reaction on an amplification reaction product, or carrying out lateral flow chromatography test strip reaction on the amplification reaction product, and then judging whether the nematode to be detected is the corn cyst nematode or not. According to the LAMP method, the corn cyst nematode can be quickly, simply, conveniently and directly detected according to the color of a reaction system and the number of indication lines of the lateral flow chromatography test strip. The primer group disclosed by the invention is strong in specificity and high in sensitivity, the detection threshold value of a single cyst of the corn cyst nematode is 1 * 10 <-4 > cyst, and the detection threshold value of a single larva is 1 * 10 <-3 > larva.

The invention discloses an RPA (recombinase polymerase amplification) specific primer group, a detection kit and a detection method for detecting corn cyst nematode. The method comprises the following steps: carrying out isothermal amplification reaction by taking genome DNA of a to-be-detected nematode as a template and adopting a specific primer group; and after the reaction is finished, carrying out agarose gel electrophoresis on an amplification product, or carrying out lateral flow chromatography test strip reaction on an amplification reaction product, or observing whether the reaction product generates fluorescence or not through visual observation to judge whether the to-be-detected nematode is the corn cyst nematode or not. The method combining RPA with LFD and CRISPR / Cas12a can rapidly, simply and directly detect the corn cyst nematode according to the number of indication lines of a lateral flow chromatography test strip and fluorescence emitted by a reaction product. The primer group disclosed by the invention is strong in specificity and high in sensitivity, the detection threshold value of a single cyst of the corn cyst nematode is 1 * 10 <-4 > cyst, and the detection threshold value of a single larva is 1 * 10 <-3 > larva.

U I T T R E K S E L Disclosed are a method and system for classifying species of necrophagous fly larvae, which can effectively overcome the problems of changeable image size and obvious noise interference in practical application, and enhance the perception ability of a model to different resolution and scale features through multiscale standardization processing and feature fusion mechanism. The introduced dual—branch feature extraction structure and a channelspatial attention mechanism can collaboratively capture local detailed features and global context information of larvae posterior surfaces, and. adaptively weight and. highlight feature regions that contribute the most to classification, significantly improving the representativeness of feature expression and discriminant power. The weighted cross—entropy loss function and label smoothing technology used effectively alleviate a model deviation that may be caused by the imbalance of category samples, improve the generalization performance and classification robustness of the model, and finally realize high—precision and high-efficiency automated classification and identification of fly larvae species. (+ Fig. l)

The invention discloses application of maltol in prevention and treatment of plant parasitic nematodes, and relates to the field of biological prevention and treatment of crop diseases, in particular to application of maltol in prevention and treatment of plant parasitic nematodes. The invention aims to solve the technical problem of environmental pollution easily caused by existing chemical agent prevention and treatment. The invention discloses application of maltol in preventing and treating plant parasitic nematodes. The maltol is used for preventing and treating the plant parasitic nematodes. The maltol is 3-hydroxy-2-methyl-4-pyrone, and the maltol is 3-hydroxy-2-methyl-4-pyrone. When the concentration of the 3-hydroxy-2-methyl-4-pyrone is 1.6 g / L, the 24-hour corrected death rate of second-instar larvae of the meloidogyne incognita reaches 90.39%, the LC50 value is 0.46 g / L, the 72-hour corrected death rate reaches 99.61%, and the LC50 value is reduced to 0.023 g / L when the concentration of the 3-hydroxy-2-methyl-4-pyrone is 96 h. When the concentration is 1.6 g / L, the 24-hour corrected death rate of second-instar larvae of the soybean cyst nematode reaches 79.54%, the LC50 value is 0.998 g / L, the 72-hour corrected death rate reaches 91.69%, and the LC50 value is reduced to 0.026 g / L when the concentration is 96 h.

This invention discloses a microbial combination with both disease prevention and growth promotion effects, along with its co-cultivation and application. Addressing the issues of insufficient synergistic effect and the need to improve the biocontrol and growth-promoting properties of existing co-cultivation technologies, this invention screens a specific strain combination (*Polyspora pinkis* NF-06 and *Bacillus belyssus* YB-1652) and employs a corresponding co-cultivation process: a specific semi-synthetic culture medium with soybean meal as the main nitrogen source, followed by simultaneous inoculation and culture. This process stably stimulates the synergistic interaction between the two strains, resulting in a product with a mortality rate of over 95.3% against second-instar larvae of the southern root-knot nematode, and significantly promotes the growth of crops such as tomatoes, achieving a synergistic effect of controlling root-knot nematodes and promoting plant growth.

The utility model provides a root knot nematode egg grain rapid disinfection and larva separation device which comprises a water containing barrel, a nematode collecting funnel is installed at the bottom of the water containing barrel, a cut-off rubber flexible rubber hose is installed at the bottom of the nematode collecting funnel, a water stop clamp is installed on the cut-off rubber flexible rubber hose, and the water stop clamp is connected with the water containing barrel. An oocyst collecting net and an egg grain collecting net which are distributed side by side up and down at intervals are mounted in the water containing barrel. The rapid disinfection and larva separation device for the root-knot nematode eggs is simple in structure, reasonable in design and convenient to use, a large batch of clean root-knot nematode larva suspension liquid with the same insect age can be collected in a short time, and tests such as toxicity determination and agent screening of various nematode killing agents can be conveniently and rapidly completed in a laboratory.

The invention relates to the technical field of microorganisms, and discloses Pseudomonas aeruginosa HNHNPg-255 and application of the Pseudomonas aeruginosa HNHNPg-255. The strain is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 66853. And the fermented supernatant can prevent and treat the Hami melon root knot nematode disease caused by the elephant trunk bean root knot nematode. Experiments show that the fermented supernate has a strong poisoning effect on second-stage larvae of the root-knot nematode and can remarkably inhibit incubation of egg masses and egg granules. In addition, the fermented supernate has good thermal stability and storage stability, and still keeps more than 80% of nematicidal activity after being stored for a long time at the high temperature of 121 DEG C and the temperature of 4 DEG C. Meanwhile, fermentation supernate of the strain can promote growth of Hami melon plants and improve physiological indexes such as root length, stem length, fresh weight and dry weight, and the strain has the application potential of being developed into efficient, environment-friendly and multifunctional biological pesticides.

The invention relates to the technical field of molecular biology, in particular to siRNA for interfering a codling moth CmHsp20-12 gene as well as a synthesis method and application of the siRNA. The nucleotide sequence of the siRNA is as shown in SEQ ID No. 1 to 2. The siRNA is sent into the body of the codling moth larva, so that the mRNA expression level of the CmHsp20-12 gene can be obviously reduced, and the gene can be effectively silenced. After gene silencing, the tolerance of the codling moth larva to high temperature stress is obviously reduced, and the death rate is obviously increased, which indicates that the CmHsp20-12 gene plays a key role in heat stress response and tolerance regulation, and provides a new molecular target and technical approach for green prevention and control of codling moth.