Rapid inspection for nematode larva of dormant pine wood

Abstract

A quick quaratine method of dormant nematoda larva in pine wood includes cutting wood into pieces and packing them by etamine to put it in container with culture solution, placing the container at 20-41 deg.c for separation culture, filtering the solution with 100 M and 500M screen mesh and collecting nematode from the solution by 500m screen mesh for being detected with microscope. The culture solution is prepared as using hydrogen peroxide as base solution then adding one or multiple of nutrition solution, gibberellin, potassium nitrate and clenbuterol hydrochloride for combination.

Description

technical field [0001] The invention relates to a rapid quarantine method for dormant pine xylophilus larvae. Background technique [0002] Pine wood nematode (also known as pine wilt) is a devastating disease on pine trees. The nematode mainly enters the xylem through the wound of Monochamus alternatus when supplementing nutrients, parasitizes in the resin duct, moves when it reproduces in large numbers, and spreads all over the whole plant, causing the destruction and death of the parenchyma cells and epithelial cells of the resin duct, resulting in plant loss. With water, transpiration decreases, resin secretion decreases and stops sharply, pine needles turn from gray-green to yellowish-brown or reddish-brown, and finally the whole plant dies. The host plant can die 40 days after being infected with pine wood nematode, and it only takes 3-5 years from the onset of the plant to the destruction of the entire pine forest. Caught off guard, once it happens, there is no radi...

AI Technical Summary

Problems solved by technology

In order to prevent pine wood nematodes from being introduced into and out of the country with wooden packaging, since 1999, various entry-exit inspection and quarantine agencies in China have strengthened the quarantine work on incoming and outgoing wooden packaging, and forest plant quarantine stations across the country have also strengthened their dispatch to epidemic areas. Inspection and quarantine work on wooden packaging of goods, but it was found during the quarantine that the current quarantine method takes a long time, ranging from 4-5 days (for materials with high nematode content) to more than 10 days (for materials with low nematode content). It is far from meeting the needs of ports and domestic forest plant rapid inspection and quarantine. The reasons are: 1. Most of the dispersive third-instar larvae that are directly separated from wood packaging materials are identified mainly based on the morphological characteristics of pine xylophilus adults. , it takes a certain amount of time to cultivate dispersed third-instar larvae or persistent fourth-instar larvae into adults; 2. Depending on the storage time, infection degree, water content, and quarantine treatment of imported wood packaging materials, the During the quarantine process, there are often few isolated pine wood nematodes, some of which are only a few. To transform these few dormant larvae into adults for identification, according to existing methods and technologies, it takes longer The time (more than 10 days) will bring a lot of trouble to ports, wharves, checkpoints, cargo owners, and testing agencies. If you have to wait until the quarantine results come out before customs clearance and release, it will cause a backlog of ships in the port, which will directly affect foreign and domestic trade; If you do not wait for the quarantine results to come out and adopt the method of customs clearance before quarantine, it may cause missed inspections or the spread of the epidemic; if the suspicious nematodes are not further inspected during the quarantine, they are judged as general pests, which may cause pine wood nematodes. If the suspicious object is determined to be pine wood nematode, it may cause trade disputes and affect the relationship between countries and domestic and foreign trade