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Drug controlling plant nematodes

A technology for nematodes and plants, applied in the direction of nematicides, plant growth regulators, insecticides, etc., can solve problems such as economic losses, unsatisfactory control, and tree death, so as to recover economic losses, increase the effect of prevention and control, and avoid Harmful effect

Active Publication Date: 2015-12-16
北京泰克美高新技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] (1) Tree protection chemicals not only require a lot of labor, but also are expensive
[0007] (2) Manual felling will lead to the direct death of trees and cause economic losses
[0008] (3) The effectiveness of pesticides has decreased in recent years
[0009] (4) Due to the different hatching and escape times of Alchaeta alternata each year, it is difficult to determine the appropriate time to apply insecticides
[0011] (6) Most of the chemicals used are high-concentration chemicals, which will remain in the soil and water systems and have an impact on animal and plant ecosystems
[0012] (7) Cannot form effective long-term governance means
[0013] Some agents for chemical control cannot be popularized due to high cost, and some widely used agents are also banned or stopped developing due to environmental problems
Most of the existing chemical agents face many problems such as high cost, unsatisfactory control and even phytotoxicity and disease resistance.

Method used

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  • Drug controlling plant nematodes
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of pesticides against nematodes

[0046] Bacterial solution preparation: Add 50g of chitin and 50g of antibiotic lysobacterial preservation solution to 50L of water, keep heating at a constant temperature of 30°C with a heating rod with a power of 800W, and continuously supply air with an oxygen pump with a power of 100W, and cultivate for 3 days. Add 50g of supporting vitamin solution, 300g of sugar, and 500g of compound fertilizer, continue heating at a constant temperature of 30°C with a heating rod with a power of 800W, and continuously feed air with an oxygen pump with a power of 100W, and cultivate for 3 days to obtain a bacterial solution.

[0047] The antibiotic lysobacterium strain used was the antibiotic lysobacterium strain HS124, which was purchased from the American Type Microorganism Culture Collection (ATCC), and the standard preservation number of the strain was ATCC29480.

[0048] Preparation of Sapinberry extract: dry and pulver...

Embodiment 2

[0050] Example 2 : Biological bacteria culture experiment.

[0051] Bacteria solution was taken and mixed with Sapindus extract in proportions of 1:1, 10:1, 100:1, 200:1 and 300:1 as the experimental group. : 1 mixed Sapindus group, and bacteria liquid group. The experimental group and the control group were respectively added to the aseptically treated solid medium with the same composition, and cultured under conditions suitable for the growth of antibiotic lysobacteria. After 7 days, the growth of microorganisms in each group was observed, and it was found that except for the Sapindus group, each group grew colonies, which showed that Sapindus had no inhibitory effect on the growth of the test bacteria.

[0052] According to the above ratio, the commercially available saponin, tea saponin, saikosaponin, pokeweed saponin, ursolic acid, prunella vulgaris saponin and begonia saponin were tested respectively, and the results showed that the growth inhibitory effect on the an...

Embodiment 3

[0053] Example 3 : nematode killing experiment

[0054] Experimental samples: The experiment was divided into four groups, and each sample was set up with 3 repetitions.

[0055] Group A: Sapindus chinensis extract group, the concentrations of sapindus saponin were 1g / L for A1 sample, 50g / L for A2 sample, 100g / L for A3 sample, and 200g / L for A4 sample;

[0056] Group B: Bacteria solution group, the concentration was 1×10 7 pcs / mL, B2 sample 5×10 7 pcs / mL, B3 sample 1×10 8 a / mL;

[0057] Group C: the mixed solution of the bacterial liquid of Example 1 and the extract of Sapindus chinensis, sample C1: 50 g / L of sapindus saponin + antibiotic lysobacterium 1×10 8 pcs / mL, C2 sample: Sapindus saponin 100g / L+ antibiotic lysobacterium 5×10 7 pcs / mL, C3 sample: Sapindus saponin 100g / L + antibiotic lysobacterium 1×10 7 a / mL;

[0058] Group D: pure water blank control group.

[0059] Experimental plant nematode: pine xylophilus.

[0060] Experimental procedure: the liquids of ...

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Abstract

The invention discloses a drug controlling plant nematodes. Effective ingredients comprise natural saponin, and the natural saponin is one or more of soapnut saponin, gleditsioside, tea saponin, saikosaponin, esculentoside, ursolic acid, vulgarsaponin and begonia yunnanensis Levl. saponin. Preferably, the drug also comprises lysobacter antibioticus, possseses relatively good killing effect on plant nematodes, nematode host cerambycidae ovum and larva and the like, and additionally, is extremely excellent in controlling effect on fruit tree gummosis. The exploited drug is environment-friendly type, is increased in controlling effect, also avoids environment harm caused by usage of a large amount of severely toxic pesticides, and possesses great reality meaning for protecting our country forest resource and ecological environment and recovering economic loss.

Description

Technical field [0001] The present invention involves the field of plant protection technology, and specifically involves a pharmaceutical prevention and treatment of plant nematode. Background technique [0002] Plant parasites are one of the original plant invasion of plant invasion. They are widely parasitic on the branches, roots, stems, leaves, flowers, buds and seeds of various plants.Here comes severe harm.In addition to directly causing damage and plunder nutrition, the pathogenic effect of nematodes on plants is mainly because the saliva secreted by nematodes during puncture contains many enzymes or toxins, which induce various pathological changes in the host tissue and cause various lesions.For example, the plant tissue cells can excessively develop, form giant cells, or dissolve the colloid layer in the cells, cause cell decomposition, and be destroyed by the cell wall, and then cause the plant root and epidermal layer to form empty cavities and even cells. [0003] P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P5/00A01P3/00A01P7/04A01N43/16
Inventor 王兵高鑫磊戴学良刘欣怡吕伟青
Owner 北京泰克美高新技术有限公司
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