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Soybean cyst nematode Hg-flp-1 gene, and encoding protein and application thereof

A technology of hg-flp-1, soybean cyst nematode, which can be applied in application, nematicide, genetic engineering and other directions, and can solve problems such as environmental pollution of soybean cyst nematode

Active Publication Date: 2020-08-25
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problem of environmental pollution in the existing chemical control methods of soybean cyst nematodes, and provides soybean cyst nematode Hg-flp-1 gene and its encoded protein and its application

Method used

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  • Soybean cyst nematode Hg-flp-1 gene, and encoding protein and application thereof
  • Soybean cyst nematode Hg-flp-1 gene, and encoding protein and application thereof
  • Soybean cyst nematode Hg-flp-1 gene, and encoding protein and application thereof

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specific Embodiment approach 1

[0019] Specific embodiment 1: The nucleotide sequence of the soybean cyst nematode Hg-flp-1 gene in this embodiment is shown in SEQ ID NO: 1 in the sequence listing.

specific Embodiment approach 2

[0020] Specific embodiment 2: The amino acid sequence of the encoded protein of soybean cyst nematode Hg-flp-1 gene in this embodiment is shown in SEQ ID NO: 2 in the sequence table.

specific Embodiment approach 3

[0021] Specific embodiment three: In this embodiment, a dsRNA fragment is designed and synthesized according to the Hg-flp-1 gene of the soybean cyst nematode, as shown in SEQ ID NO: 3 in the sequence listing.

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Abstract

The invention discloses a soybean cyst nematode Hg-flp-1 gene as well as an encoding protein and application thereof, and relates to a soybean cyst nematode neuropeptide gene as well as an encoding protein and application thereof. The invention aims to solve the problem of environmental pollution in the existing chemical control method of soybean cyst nematode. The nucleotide sequence of the geneis shown as SEQ ID NO: 1 in a sequence table. The amino acid sequence of the encoded protein is shown as SEQ ID NO: 2 in a sequence table. After the neuropeptide gene Hg-flp-1 is subjected to dsRNA treatment, the number of second-stage instar larvae in infected roots and the number of root surface female insects after 21 days of infection are reduced by 25% and 28.9% respectively. The soybean cystnematode Hg-flp-1 gene disclosed by the invention is applied to the field of soybean cyst nematode prevention and control.

Description

technical field [0001] The invention relates to a soybean cyst nematode neuropeptide gene, its encoded protein and its application. Background technique [0002] Soybean is an important grain and oil crop, and also the main source of plant protein. The quality and yield of soybean are crucial to the development of my country's agricultural economy. Soybean cyst nematode is one of the important factors restricting the quality and yield of soybean. The disease occurs everywhere in my country where soybean is produced. Soybean cyst nematode is an endoparasitic nematode that mainly parasitizes in leguminous plants. SCN invades through the root system of the host plant, causing the growth of the host root to be stunted, root nodules to be reduced, and in severe cases, it will lead to extinction; in addition, soybean cyst nematode infection will intensify Incidence of Phytophthora soybean, soybean stem brown rot (Sugawara, 1997; Kaitany, 2000; Tabor, 2003). At present, the contr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/435C12N15/113A01N63/60A01P5/00
CPCA01N63/60C07K14/4354C12N15/113C12N2310/14
Inventor 尤佳王从丽胡岩峰
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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