Root-knot nematode lure attraction agent and application thereof

Abstract

The invention relates to a root-knot nematode lure attraction agent and application thereof, and belongs to the technical field of plant disease prevention and treatment. The biological lure attraction agent is obtained from Bacillus methylotrophicus Mo-TB strain with the culture preservation number being CGMCC No.11851 through the steps of test tube slant seed culture, liquid culture, fermentation pot mass culture and lure attraction agent preparation. The lure attraction agent is applied to lure attraction of root-knot nematode second-stage larvae in soil. The biological lure attractive agent and abamectin and fosthiazate chemical pesticides are mixed and then applied in prevention and treatment of the root-knot nematode disease. The root-knot nematode lure attraction agent has the advantages that the lure attraction rate of the biological lure attraction agent for root-knot nematode second-stage larvae in soil within one centare is 80% or above; the effect of preventing and treating the root-knot nematode disease is improved due to the fact that the biological lure attraction agent is used by being mixed with abamectin and fosthiazate chemical pesticides; the biological lure attraction agent is low in cost, free of residue and safe for human and livestock, and the prevention effect of conventional nematode killing pesticide can be significantly improved.

Description

Technical field: [0001] The invention relates to a root-knot nematode attractant and an application thereof, belonging to the technical field of plant disease prevention and control. Background technique: [0002] Root-knot nematode (Meloidogynes pp.) is one of the important pathogens of various crops. The annual economic loss caused by root-knot nematode on crops worldwide is as high as 100 billion US dollars (Oka et al., 2009). In China, in recent years, with the expansion of facility vegetable cultivation area, the damage of root-knot nematodes has become more and more serious due to reasons such as continuous cropping, closed facility environment, high temperature and high humidity, resulting in a 30-50% reduction in vegetable production, and in severe cases, even no harvest (Wang Yanyan et al., 2014 ). In the hundreds of thousands of acres of greenhouse tomato planting in Shouguang, Shandong, even with the application of large doses of chemical nematicides, the root-k...

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Problems solved by technology

[0006] Bacillus methylotrophicus is a bacterium that can metabolize methanol and promote plant growth reported by Koreans in 2010 as a new species (Madhaiyan et al., 2010). ); but the attracting function of the microorganism to root-knot nematodes and its application in nematode control have not been publicly reported so far

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