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Arthrobotrys oligospora strain N and application thereof

A technology of Arthroxospora oligospores and Arthroxospora oligospores, which is applied in the fields of application, fungi, plant growth regulators, etc., and can solve problems such as hindering the popularization and application of Arthrobacter oligospores, low yield, and slow speed , to achieve the effects of good practicability, fast growth and reproduction, and early spore production time

Inactive Publication Date: 2012-07-25
INNER MONGOLIA UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing Arthropodium oligosporum reproduces and forms spores at a relatively slow speed, and the spores begin to be produced after 5 to 7 days, and it takes 3 weeks for a large number of spores to be produced. , the output is also very little, and the predation rate of the third-stage larvae of the nematodes is about 80%, which hinders the popularization and application of Arthrobusporum oligospora to a certain extent.

Method used

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  • Arthrobotrys oligospora strain N and application thereof
  • Arthrobotrys oligospora strain N and application thereof
  • Arthrobotrys oligospora strain N and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 Low-energy nitrogen ion beam mutagenesis Arthrobuspora oligospora

[0024] 1) 0.4g / L corn flour agar medium. Weigh 40g of fresh corn flour, add 1000mL of distilled water, boil on low heat for 1 hour, make up water to 1000mL, filter with gauze, take 10mL of filtrate, add 990mL of distilled water and mix well, adjust the pH value to 6.0-6.2, add 25g of agar powder, After autoclaving, pour into sterilized glass Petri dishes for later use.

[0025] Preparation of starting strains: inoculate Arthrobuspora oligospora strains on 0.4g / L corn flour agar medium, and cultivate them in an incubator at 25°C±1°C.

[0026] Preparation of spore eluent: Take 1000mL distilled water, add 2mL Tween-80, heat and mix well, and distribute in 500mL glass bottles, 1×10 5 Pa sterilized for 20 minutes and then ready for use.

[0027] After culturing for 3 weeks, add the spore eluate, stir with a sterilized glass rod, wash 3-5 times with 3-5mL each time, elute the conidia, collect ...

Embodiment 2

[0030] Embodiment 2 Low-energy nitrogen ion beam mutagenesis Arthrobuspora oligospora strain spore culture

[0031] Strain expansion: thawed spores were inoculated on 0.4g / L corn flour agar medium. Set at 25±1°C and culture for 7 days.

[0032] Preparation of spore medium: Clean 250g of fresh corn kernels, put them into a 500mL Erlenmeyer flask, add 250mL of distilled water, soak at room temperature for 24 hours, and then sterilize under high pressure at 15 pounds for 25 minutes.

[0033] Spore culture: Cut off the mixture of mycelium and spores in 0.4g / L corn flour agar medium along with the agar block, inoculate it on corn grain medium, and culture it in an incubator at 20°C ± 1°C for 3 weeks. It was observed that the Arthrobuspora oligospora strain N obtained by mutagenesis in Example 1 began to germinate in about 4 hours on a 0.4 g / L corn flour agar medium. The mycelium width is between 3 μm and 7 μm, and the average daily mycelial growth rate is about 10 mm / d. Vertical...

Embodiment 3

[0034] Example 3 Preservation of low-energy nitrogen ion beam mutagenesis of Arthrobacter oligospora strain spores

[0035] Spore culture: Cut off the mycelial spore mixture of Arthrospora oligospora growing on the corn flour agar medium along with the agar block, inoculate it on the corn kernel medium, and culture it in an incubator at 20°C ± 1°C for 3 weeks.

[0036] Extraction and preparation of preserved spores: elute conidia with 0.02% Tween-80 solution, and prepare component sporozoite suspension. Take the suspension and add it to a cryovial, centrifuge at 3000r / min for 10min, discard the supernatant, and add protection solution.

[0037] Preservation of spores: In ordinary refrigerators -18°C freezer, -30°C freezer, -70°C low-temperature freezer or -195°C liquid nitrogen tank, after half a year or one year of frozen storage, the conidia still retain their vitality.

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Abstract

The invention provides an arthrobotrys oligospora strain N, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.5508. The strain is a mutagenic strain obtained by injecting low-energy nitrogen ion beams into arthrobotrys oligospora. The strain has the characteristics that the growth and reproduction are quick, spore production is early, the quantity of produced spores is large, and the capacity for catching and feeding on third-stage larva of nematode is strong, so that the strain can be used for biological control on livestock parasitic nematode.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an Arthrobotrys oligospora strain N capable of predating nematodes and an application thereof. Background technique [0002] Parasitic diseases, especially parasitic nematodes, are still serious enemies to the development of animal husbandry. The control of such pathogenic organisms mainly relies on anti-helminth chemicals, but the long-term and frequent use of chemical anthelmintic drugs inevitably leads to problems such as drug resistance, drug residues, and ecological environment pollution. In order to solve the above-mentioned disadvantages brought about by chemical anthelmintic drugs in the prevention and treatment of livestock parasitic nematodes and promote the sustainable development of animal husbandry, it is urgent to seek new methods for the prevention and treatment of livestock nematodes. At present, the most researched, the most in-depth and the most promising applicati...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N13/00C12N15/01A23K1/16A01N63/04A01P5/00C12R1/645
Inventor 王军杨晓野王瑞杨莲茹
Owner INNER MONGOLIA UNIVERSITY
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