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Biological medicament for preventing and treating plant parasitic nematode and uses thereof

A biopharmaceutical and plant technology, applied in the direction of plant growth regulators, biocides, nematicides, etc., can solve problems such as high toxicity, serious pollution, and environmental damage

Active Publication Date: 2011-04-20
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of plant parasitic nematodes is still dominated by chemical control, but there are few varieties of chemical nematicides, and most of them are highly toxic and high residue, which seriously pollute and damage the environment. With the improvement of people's awareness of environmental protection and the demand for safe agricultural products demand, biological control methods in line with the strategic concept of sustainable development of modern agriculture have been paid more and more attention, and have become the first choice for root-knot nematode control

Method used

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  • Biological medicament for preventing and treating plant parasitic nematode and uses thereof
  • Biological medicament for preventing and treating plant parasitic nematode and uses thereof
  • Biological medicament for preventing and treating plant parasitic nematode and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1. Isolation, purification and identification of Streptomyces rubrogriseus HDZ-9-47

[0018]Meloidogyne incognita (Meloidogyne incognita) eggs of a certain concentration (collected from the diseased roots of tomatoes infected by Meloidogyne incognita in the experimental greenhouse of the Nematology Laboratory of China Agricultural University. Taylor A L., Sasser J N. Edited. Yang Baojun , Zeng Dapeng translation. Plant root-knot nematode biology, taxonomic identification and control. Beijing: Science Press, 1983) suspension is coated on the PDA medium (about 300 / dish), the female worm that hatches is carried out disinfection , crushed the sterilized female worms, inoculated them on PDA medium plates, and cultivated them at 28°C. After the single colony grows, pick the single colony into the ISP2 medium (the composition of the ISP2 medium is: yeast extract 4.0g / L, malt extract 10.0g / L, glucose 4.0g / L, agar 20.0g / L , the rest is water, pH 7.2), cultured and purif...

Embodiment 2

[0022] Embodiment 2, utilize Streptomyces rubrogriseus (Streptomyces rubrogriseus) HDZ-9-47CGMCC №2878 and its metabolites to carry out the control of plant parasitic nematodes

[0023] 1. Culture of plate strains

[0024] The formula of the medium is Gaoshi Synthetic No. 1 Agar Medium (GA), and its specific composition is: KNO 3 1g / L, K 2 HPO 4 0.5g / L, MgSO 4 0.5g / L, NaCl 0.5g / L, FeSO 4 0.01g / L, soluble starch 20g / L, agar 20g / L, the rest is water; pH value is 7.2-7.4.

[0025] Inoculate Streptomyces rubrogriseus (Streptomyces rubrogriseus) HDZ-9-47CGMCC № 2878 screened in the above-mentioned Example 1 onto the above-mentioned Gaoshi Synthetic No. 1 agar medium plate, culture at 28°C for 7 days, pick a single colony, and obtain a plate strains.

[0026] 2. Liquid fermentation culture

[0027] The composition of the fermentation medium is: 4% soybean powder, 4% starch, 0.2% dipotassium hydrogen phosphate, 0.05% sodium chloride, 0.2% calcium carbonate, 0.1% ferrous su...

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Abstract

The invention discloses a biological medicine for preventing and curing plant parasitic nematode and application thereof. The active ingredient of the biological medicine is Streptomyces rubrogriseus HDZ-9-47 CGMCC No 2878. The biological medicine in the invention prevents and cures the plant parasitic nematode, and the results show that the Streptomyces rubrogriseus HDZ-9-47 CGMCC No 2878 and metabolite thereof have very strong toxic activity for eggs and second instar larva of Meloidogyne incognita and can obviously inhibit hatching of the eggs, and have obvious parasitization for the eggs and egg capsules of the Meloidogyne incognita; when the Streptomyces rubrogriseus HDZ-9-47 CGMCC No 2878 and the metabolite thereof are mixedly used, the effect of insect disinfestation on the Meloidogyne incognita can reach 100%. The greenhouse experimental results of tomatoes show that the Streptomyces rubrogriseus HDZ-9-47 CGMCC No. 2878 has very good nematicidal effect. The biological medicine in the invention has good application development prospect for prevention and cure of the plant parasitic nematode.

Description

technical field [0001] The invention relates to a biological agent for preventing and controlling plant parasitic nematodes and its application. Background technique [0002] Plant parasitic nematodes are an important class of plant pathogens, which occur and cause damage in many countries and regions in the world. There are many kinds of plant parasitic nematodes, more than 200 genera and more than 5000 species of plant parasitic nematodes have been reported in the world. Among them, root-knot nematode is the most harmful type of plant parasitic nematode in agriculture. It has a wide distribution area and diverse hosts, and can parasitize more than 2000 different economic crops such as oil crops, vegetables, tea, fruit trees, tobacco and Chinese medicinal materials. According to statistics, the root-knot nematode causes an annual loss rate of about 5% of the world's major crops, and the economic loss exceeds 50 billion US dollars. With the continuous adjustment of my coun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P5/00C12R1/465
Inventor 简恒薛慧刘杏忠刘倩
Owner CHINA AGRI UNIV
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