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A strain of Bacillus pumilus and its application in the control of cereal cyst nematode in wheat

A technology of Bacillus pumilus and cereal cyst nematode, which is applied to a strain of Bacillus pumilus and its application field in preventing and treating wheat cereal cyst nematode, can solve the problems of ineffective biocontrol preparations and the like, and is not easy to achieve. The effect of drug resistance, low production cost, human and animal safety

Active Publication Date: 2014-10-29
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there have been some studies at home and abroad on the types, screening, control effects and soil additives of plant parasitic nematode biocontrol bacteria, there is no biocontrol agent with stable effect in production.

Method used

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  • A strain of Bacillus pumilus and its application in the control of cereal cyst nematode in wheat
  • A strain of Bacillus pumilus and its application in the control of cereal cyst nematode in wheat
  • A strain of Bacillus pumilus and its application in the control of cereal cyst nematode in wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1, the acquisition and identification of Bacillus pumilus B202 bacterial strain

[0023] 1. Obtaining of Bacillus pumilus B202 strain

[0024] Severely diseased wheat and healthy wheat were excavated from plots with serious incidence of cereal cyst nematodes in Henan, Hebei, and Shaanxi provinces, the main wheat producing areas in the central plain of China, and the root soil was preserved. Carefully remove the soil at the root of the wheat plant, randomly cut out new tap roots and capillary roots, place them in 50ml of sterile water containing glass beads, and shake them well. The roots are removed to obtain a soil suspension. The root system taken out was first rinsed with sterile water, then sterilized with 1% sodium hypochlorite for 8 minutes, and then rinsed with sterile water for 4 times to rinse off residual sodium hypochlorite. Mix 2 grams of root with 10ml of sterile water and add a small amount of quartz sand to the sterile water. Grind in a funga...

Embodiment 2

[0031] Embodiment 2, the cultivation of bacillus pumilus B202

[0032] 1. Inoculate a single colony of Bacillus pumilus B202 into NA modified medium (solvent is water, containing 3g / L beef extract, 5g / L peptone, 3g / L glucose; pH7.0), shake at 30°C and 180rpm / min Cultivate for 48 hours to get OD 600nm =4.0 fermentation system suspension.

[0033]2. Centrifuge the suspension of the fermentation system obtained in step 1 at 4000 rpm for 30 minutes, and collect the supernatant and bacterial precipitates respectively.

[0034] 3. Suspend the bacterial cell precipitate obtained in step 2 in sterile water to obtain a spore concentration of 8×10 8 spore suspension per ml.

Embodiment 3

[0035] Example 3, Influence of Bacillus pumilus B202 on the activity of wheat cereal cyst nematode under indoor conditions

[0036] 1. Suspending the second-instar larvae of wheat cereal cyst nematodes with sterile water.

[0037] 2. Group processing is as follows:

[0038] Experimental group: 250 μl of the suspension obtained in Step 1 (containing 100±5 second-instar larvae of wheat cereal cyst nematodes) was mixed with 250 μl of the fermentation system suspension obtained in Example 2, and cultured at room temperature for 24 hours.

[0039] Control group: 250 μl of the suspension obtained in step 1 (containing 100±5 second-instar larvae of wheat cereal cyst nematodes) was mixed with 250 μl of sterile water, and cultured at room temperature for 24 hours.

[0040] 3. Add 1mol / L NaOH aqueous solution to each culture system in step 2 (the surviving larvae will move under alkaline stimulation, and the immobile larvae will be counted as dead larvae), and count the number of secon...

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Abstract

Disclosed are a Bacillus pumilus strain and the use thereof in preventing and treating cereal cyst nematode in wheat, which strain is a Bacillus pumilus named B202 and deposited in CGMCC with a deposit number of CGMCC NO. 5838. Also disclosed is the use of the Bacillus pumilus strain B202 in the preparation of a biological protective preparation for cereal cyst nematode in wheat. The active ingredients of the biological protective preparation for cereal cyst nematode in wheat comprise at least one of the following (a) or (b) or (c) or (d): (a) a fermentation culture of the Bacillus pumilus strain B202; (b) the Bacillus pumilus strain B202; (c) spores of the Bacillus pumilus strain B202; and (d) a fermentation supernatant of the Bacillus pumilus strain B202. The Bacillus pumilus strain B202 has a stable prevention and treatment effect on cereal cyst nematode in wheat.

Description

technical field [0001] The invention relates to a strain of bacillus pumilus and its application in preventing and treating wheat cereal cyst nematode disease. Background technique [0002] Cereal cyst nematode, also known as oat cyst nematode (Heterodera avenae Wollen-weber 1924, Cereal Cyst Nematodes, CCN for short), is a worldwide pathogenic nematode of cereal crops. Harm to wheat, and increase year by year, become the main disease of wheat production. At present, the control of wheat cereal cyst nematode is mainly chemical control and the utilization of disease-resistant varieties. Chemical nematocides with good control effects, such as Temik and Furadan, are highly toxic pesticides, which have problems such as high toxicity, high cost, environmental pollution, and human health hazards. Cannot be promoted. Breeding new varieties resistant to cereal cyst nematodes is the most effective way to control root nematode diseases, but the identified genes resistant to cereal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P5/00C12R1/07A01N63/22
CPCC12N1/20A01N63/00C12R1/07A01N63/02A01N63/22C12R2001/07C12N1/205
Inventor 王琦李洪涛李燕
Owner CHINA AGRI UNIV
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