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Simple inoculation device for wheat powdery mildew and its application method

A technology of wheat powdery mildew and an inoculation device, which is applied to the methods of sampling biological materials, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of the drop of spore powder in the middle of the shield, the cost of the instrument is high, the spore powder is too much, etc., and the inoculation is achieved. Uniform effect, low installation cost, simple and easy to use effect

Inactive Publication Date: 2017-12-08
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]According to literature review, "Jiangsu Agricultural Science" 1986, No. 05 published "Simple Powdery Mildew Inoculation Tower", the middle part of the tower is equipped with an upward blowing electric fan, The bottom is equipped with a rotatable storage table. When in use, the diseased leaves are shaken from the tower mouth at the top, so that they are blown away and then fall to the host plant model on the storage table. The disadvantage is that the airflow blown upward by the electric fan will form The rotating airflow moving in a circle, the spore powder rotating with the airflow tends to settle along the surrounding wall of the tower body, resulting in more sedimentation spore powder around the storage table, and the distribution of sedimentation along the radial direction is very uneven, and the electric fan blocks the spore powder in the middle Decline
Another example is "an inoculation device CN 203735134 U" developed by the Nanjing Agricultural Mechanization Research Institute of the Ministry of Agriculture. There are at least three upwardly inclined pneumatic nozzles that are uniform in the circumferential direction. The jet diffusion angle of the pneumatic nozzles forms an air flow confluence area that can accommodate the mesh cage. Its disadvantages are that it needs to be equipped with a power supply, and the instrument is expensive. It is not suitable for small and medium-sized test samples. inoculation

Method used

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  • Simple inoculation device for wheat powdery mildew and its application method
  • Simple inoculation device for wheat powdery mildew and its application method
  • Simple inoculation device for wheat powdery mildew and its application method

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1, the inoculation test of different varieties of wheat disease resistance determination

[0018] According to the requirement of resistance identification and inoculation, 6 leaves were placed in the leaf tray. First, soak the hollow blowing rod with absolute alcohol for 1 minute and then take it out, light the remaining alcohol with a lighter; when the temperature of the hollow blowing rod drops to room temperature, put the pre-cultivated leaves with fresh powdery mildew spores Load the blisk, place the blisk into the groove and gently push the blisk to the port of the stainless steel screen.

[0019] The leaves of different wheat varieties to be tested are placed in a horizontal position, and the self-made inoculation tower (plastic bucket 15×22×22cm) is turned upside down, and one end of the hollow blowing rod (installed with a stainless steel screen) is placed in the inoculation tower In the circular orifice of the hollow blowing rod, use your mouth to ...

Embodiment 2

[0020] Embodiment 2, pesticide bioactivity assay inoculation test

[0021] According to the requirements of pesticide activity assay inoculation, 3 leaves were placed in the leaf tray. First, soak the hollow blowing rod with anhydrous alcohol for 1 minute, then take it out, light the remaining alcohol with a lighter, and when the temperature of the hollow blowing rod reaches room temperature, put the pre-cultivated leaves with fresh powdery mildew spores into the Leaf loading disc, put the leaf loading disc into the groove and gently push the leaf loading disc to the port of the stainless steel screen.

[0022] The sample to be tested is placed in a horizontal position, and the self-made inoculation tower (plastic bucket 15×22×22 cm) is turned upside down, and one end of the hollow blowing rod (installed with a stainless steel screen) is placed in the circular hole of the inoculation tower In the mouth, use your mouth to blow air against the other port of the hollow blowing r...

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Abstract

The invention discloses a simple and convenient inoculating device for blumeria graminis f.sp.tritici and an application method of the inoculating device and relates to the technical field of plant protection. The inoculating device is composed of a hollow blowing rod, a leaf carrying plate and a stainless steel screen. The stainless steel screen is arranged in one end opening of the hollow blowing rod, and a groove is formed in the position 8 cm away from the end opening. The inoculating device is easy and convenient to operate, low in cost, capable of being reused and suitable for variety disease resistance evaluation, pesticide bioassay and other work, and needs no power.

Description

Technical field: [0001] The invention relates to a wheat powdery mildew inoculation device and a use method thereof, and belongs to the technical field of plant pathogenic obligate parasite inoculation tools and its application. Background technique: [0002] The invention relates to a simple inoculation device for powdery mildew and a method for using it. Wheat powdery mildew is a disease transmitted by airflow, and the conidia of powdery mildew mainly spread with the airflow, and the bacteria cannot grow into obligate parasites on the culture medium , can only be transferred from generation to generation on fresh host plants. Therefore, when studying the law of disease occurrence, identifying the disease resistance of varieties and measuring the biological activity of pesticides, it is necessary to rely on manual inoculation to draw more reliable conclusions. [0003] According to literature review, "Jiangsu Agricultural Science" published "Simple Powdery Mildew Inoculati...

Claims

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Application Information

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IPC IPC(8): C12M1/26
CPCC12M33/00
Inventor 杨立军龚双军向礼波喻大昭薛敏峰张学江史文琦曾凡松汪华
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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