Hydrogenophagasp and application thereof to prevention and treatment of wheat powdery mildew

A technology for wheat powdery mildew and hydrogenophilus, which is applied in the field of microorganisms and their microbial applications

Active Publication Date: 2016-05-04
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On June 23, 2014, it was preserved in the General Microorganism Center (CGMCC) of the China Microbiological Culture Collection Management Committee (CGMCC)

Method used

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  • Hydrogenophagasp and application thereof to prevention and treatment of wheat powdery mildew
  • Hydrogenophagasp and application thereof to prevention and treatment of wheat powdery mildew
  • Hydrogenophagasp and application thereof to prevention and treatment of wheat powdery mildew

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Experimental program
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Effect test

Embodiment 1

[0020] The screening of embodiment 1 hydrogenophilic bacteria BB-E

[0021] A proper amount of soil samples were taken from the wheat planting area in the western suburbs of Zhengzhou by the five-point sampling method, put into sterile polyethylene plastic bags, and sealed. Separation and purification of bacterial strains by dilution plate separation method, mixing bacterial fermentation liquid with 3% agarose in a melted state (1:1) before solidification, and dripping on a concave slide to prepare a mixed culture medium for fermentation liquid. The 1.5% agar medium of wheat powdery mildew was taken as a control, and the fresh spores of wheat powdery mildew were shaken off in the medium, cultured for 48 hours, the germination rate of wheat powdery mildew spores was counted, and the inhibition rate of spore germination was calculated.

Embodiment 2

[0022] Morphological analysis and physiological and biochemical identification of embodiment 2 hydrogenophilic bacteria BB-E

[0023] Microscopic observation found that the bacterial BB-E colonies were evenly distributed, with low bulges, smooth edges, light yellow, smooth texture, moist, and translucent.

[0024] Physiological and biochemical identification of the strains, the results are shown in Table 1.

[0025] Table 1 Physiological and biochemical identification results of antagonistic bacteria BB-E

[0026]

[0027] Note: "+" means positive; "-" means negative.

[0028] According to the physiological and biochemical identification results of the bacteria, combined with the "Berger's Bacterial Identification Manual", the description of the characteristics of the hydrogen-phagic bacteria is more consistent with the bacteria BB-E, and BB-E is initially identified as a hydrogen-phagic bacteria.

Embodiment 3

[0029] The 16rDNA sequence identification of embodiment 3 hydrogenophilic bacteria BB-E

[0030] Genomic DNA was extracted, and 16SrDNA sequence universal primers were used for PCR amplification, and a 1.0% concentration of agarose gel electrophoresis was used to detect that an amplified fragment with a length of about 1500bp was the 16SrDNA sequence of the antagonistic bacterium BB-E ( figure 2 ).

[0031] The target DNA fragment was recovered, purified, cloned and sequenced to obtain a sequence of 1490bp in size. The sequence was searched and compared using the BLAST tool in the biological information database NCBI. The results are shown in image 3 . Select highly similar strains with definite species status from the BLAST comparison results, obtain their 16S rDNA sequences, and use DNAMAN software to construct a phylogenetic tree ( image 3 ).

[0032] Establish a phylogenetic tree based on these 11 strains, such as image 3 As shown, the species status among the stra...

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Abstract

The invention discloses a strain of (Hydrogenophagasp) BB-E which is separated from soil and has an antagonistic action on blumeria graminis, and the preservation serial number is CGMCC No. 9374. The strain BB-B can conduct antagonism on blumeria graminis, and it is found through detection and analysis that the BB-B can generate blumeria graminis, chitinase, cellulose and biological surfactin. It is indicated through wheat in vitro leaf segment imitation experiments that after treatment with fermentation liquid with the concentration being 50.0% is conducted, the disease index of wheat powdery mildew is 23.4%, and compared with the disease index of 95.1% of a comparison group, the disease prevention effect reaches 75.4%; a good prevention and treatment effect is achieved, and resistance to powdery mildew can be enhanced.

Description

technical field [0001] The invention relates to the field of microorganisms and the application of microorganisms. In particular, it relates to a hydrogenophilic bacterium which has antagonistic effect on wheat powdery mildew, and the fermented liquid contains antibacterial active substances, which can be used for the prevention and treatment of wheat powdery mildew. Background technique [0002] Wheat powdery mildew caused by wheat powdery mildew seriously affects the quality and yield of wheat in various wheat producing areas in the world. It can be infected during the whole growth period of wheat growth. Wheat powdery mildew is an obligate living parasitic fungus, which mainly infects wheat leaf sheaths, stems and ears. Since the 1970s, with the rapid exchange of wheat varieties in various production areas and the continuous improvement and convergence of farming systems, the distribution of wheat powdery mildew has become more and more widespread and more serious, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 伊艳杰周广舟李桂玲李瑞芳景红娟杨艳会张宗武张长付秦素雅
Owner HENAN UNIVERSITY OF TECHNOLOGY
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