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Long-term preservation method of blumeria graminis strains

A technology for long-term preservation of wheat powdery mildew bacteria, applied in the preservation of microorganisms, etc., can solve problems such as cross-contamination, short storage period, and labor-intensive, and achieve the effect of overcoming cumbersome operations, long storage time, and simple and practical methods

Inactive Publication Date: 2013-07-31
INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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Problems solved by technology

[0005] The purpose of the present invention is to propose an easy-to-operate, time-saving method for the defects of short storage period, time-consuming, labor-intensive and easy to produce cross-contamination in the frequent transfer process of the existing wheat powdery mildew pathogen in the preservation of wheat living organisms. A method that saves labor, can reduce cross-contamination between different strains, and can preserve wheat powdery mildew strains for a long time, so as to ensure the normal development of research work related to wheat powdery mildew pathogens

Method used

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  • Long-term preservation method of blumeria graminis strains
  • Long-term preservation method of blumeria graminis strains

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Embodiment 1

[0024] Embodiment 1, wheat powdery mildew bacterial strain slow drying long-term preservation

[0025] The method proceeds as follows:

[0026] 1. Collection and multiplication of monospore strains: 10 days before the collection of the strains, sow the broad-spectrum high-sensitivity wheat powdery mildew variety "Zhengmai 98" to one leaf and one heart for later use; collect leaves with only a single spore pile from the field, Cut the first leaf of the pre-prepared wheat plant to a length of 3 cm, put the leaf face up on the agar fresh-keeping medium containing benzimidazole, pick the spores with a toothpick in the ultra-clean bench and gently smear the leaves, and place them in the growth chamber. Interculture (17±1°C, 18h light) for 8-10 days, after a large number of fresh conidia are produced, they will be used as primary propagation strains for future use. Cut the first leaf of the above-mentioned wheat plant into 3 cm long leaf segments and put them on the fresh medium (p...

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Abstract

The invention discloses a long-term preservation method of blumeria graminis strains, which comprises the steps of (1) the expanding propagation of monadic strains; (2) the collection of conidium; and (3) after silica gel is slowly dried for 5h, preservation in a refrigerator at a temperature of -70 DEG C. According to the method, the preservation time of blumeria graminis strains can be longer, is increased to 1 year from 10-12d by using conventional methods, and the method is simple, convenient and practical. The defects of the conventional preservation methods that operation is complicated, manpower and material resources consumption are seirous are overcome, so that the normal implementation of related researches on blumeria graminis strains is ensured. The method disclosed by the invention can be popularized and applied to wheat disease research and breeding mechanisms.

Description

technical field [0001] The invention relates to the technical field of methods for preserving crop pathogens, in particular to a method for long-term preservation of wheat powdery mildew monospore strains. Background technique [0002] Wheat (Triticum aestivum L.) is one of the most important food crops in the world today, and its yield and quality are easily affected by diseases such as bacteria, fungi, and viruses. Among wheat diseases, powdery mildew is one of the diseases with the widest occurrence range and great impact on yield. After the wheat is harmed by powdery mildew, powdery spots appear on the leaves and ears. In severe cases, the leaves turn yellow and die early, the plants are short and thin, the photosynthesis is reduced, the respiration is strengthened, the number of tillers is reduced, the rate of ear formation is reduced, and the thousand-grain weight is reduced. It can reduce the yield by 5% to 10%, and when it is popular, it can reduce the yield of whea...

Claims

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Application Information

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IPC IPC(8): C12N1/04
Inventor 喻大昭龚双军杨立军曾凡松史文琦向礼波汪华张学江薛敏峰
Owner INST OF PLANT PROTECTION & SOIL FERTILIZER HUBEI ACAD OF AGRI SCI
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